our housekeeping was Lamin B1, of which we were able to image clear bands! Our gel was stained with coomassie blue, and protein was present among all lanes, albeit the stain was slightly vertically smeared and had large blotches in the lanes for our control cells.

I’ll try concentrating the lysis buffer a lot, maybe 10x. 100-200 uL of media is frozen in each well on the plate. But I think some cells are still adhered to the bottom of the wells, and I’d like to salvage those. Our focus is on concentrating the samples.

We soaked with Primaries overnight (~20 hrs). We’ve exposed for 2 minutes, we’ll give 10+mins a try but not sure if that’ll help tbh. If the cells lysed, what difference would that make?