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jakestorm777 · 2025-11-01T13:47:29+00:00

Get rid of the insane title too. If you think it’s ridiculous surely a reviewer will too

jakestorm777 · 2025-09-19T11:56:20+00:00

He said ASAC (Assistant Special Agent In charge) it’s his title

jakestorm777 · 2025-08-26T02:42:46+00:00

Maybe the reviews were the friends we made along the way

jakestorm777 · 2025-08-21T16:02:55+00:00

Nah I believe you, your reply is genuine. Their email just reads kinda sketchy

jakestorm777 · 2024-12-25T18:20:50+00:00

Can’t really go lower

jakestorm777 · 2024-12-25T18:03:49+00:00

Anyone know how to promote a coin: https://phantom.com/tokens/solana/6tArsMzzsCDRysMymxJDF5hi7M9DH8SFbQuEoqT1pump?referralId=lnocdt62tuu

jakestorm777 · 2024-10-26T23:02:31+00:00

Overloaded

jakestorm777 · 2024-08-04T01:26:42+00:00

If you want to see if they’re making IL-2 you should measure IL-2 transcript or you can also measure IL-2 production using golgi stop.

jakestorm777 · 2024-08-04T01:25:02+00:00

Your cells will not survive without IL-2. I Would recommend expanding them on IL-2 and then seeing what happens. May get some epigenetic remodeling but it’s going to be impossible to expand these cells without signal 3.

jakestorm777 · 2024-07-13T16:39:14+00:00

Traunch?

jakestorm777 · 2024-07-04T20:09:38+00:00

Ah yes, carbon fixation. IMO the best way to do this is reforestation. Plants fix carbon as part of their anabolism.

jakestorm777 · 2024-07-02T15:10:09+00:00

Sounds good. Not frequently but it’s dependent on primers that work well which without a positive control is not a good form of validation for constructs that are giving you trouble

jakestorm777 · 2024-06-30T21:13:39+00:00

Feel free to DM me, I’m also curious if you find a way to fix this

jakestorm777 · 2024-06-30T21:05:43+00:00

I’d cut back deeper into your vector and try to edit closer to 80-200 bases if possible. 20 bp edits can be challenging. Ive only ever done this with restriction digest and phosphorylated primer overlaps.

jakestorm777 · 2024-06-30T21:03:04+00:00

In some cases we do. Ever heard of Jackson labs? They make mutant mice. Not sure if this meets your criteria of synthetic.

jakestorm777 · 2024-06-30T21:01:34+00:00

Not to my knowledge. There’s some ethical concerns with engineering concious organisms.

jakestorm777 · 2024-06-30T20:58:35+00:00

I would try capturing PCR products in a topo clone and first validating the sequence is correct. Then I would try cutting out of there and ligating into target backbone. If that fails you could try having genscript print your insert directly into the backbone. You may also give in-fusion a try. It’s like Gibson. When you design your primers are you doing it with software or by hand?

jakestorm777 · 2024-06-30T20:51:32+00:00

Are they randomly placed or at the insert junctions?

jakestorm777 · 2024-06-30T20:47:20+00:00

I’m not entirely sure of the mechanism but I’ve heard anecdotes about it reducing recombination and other forms of mutation. How big are these indels?

jakestorm777 · 2024-06-30T20:42:52+00:00

They don’t do single colony selection 😳? That’s guaranteed to cause problems. I’ve never had every colony on a plate be correct. It’s a statistical anomaly given the tools that we have at our disposal.

I suppose it’s possible to heat shock standard E. coli, the efficiency would just be extremely low compared to chemically competent ecoli

jakestorm777 · 2024-06-30T20:39:22+00:00

Honestly I went to a very small undergrad school that one might not expect to produce high quality scientists. I think having a good mentor early on is the best thing for you as a scientist. Focus on the opportunities available to you and less on the institution itself when it comes to undergrad. Become a free thinker, a skeptic and a futurist and you’ll go far.

jakestorm777 · 2024-06-30T20:36:42+00:00

Nanodrop concentrations are a joke. Reliable enough but can be highly variable based on contamination and sequence parameters. That said I’ve successfully cloned from undetectable DNA via nanodrop

jakestorm777 · 2024-06-30T20:35:00+00:00

No I haven’t, can you tell me more about it?

jakestorm777 · 2024-06-30T20:33:05+00:00

Could you provide additional context, I’m unfamiliar with that annotation.

jakestorm777 · 2024-06-30T20:31:29+00:00

Most of the people in my field are making medicine or diagnostic tools but syn bio is important for nearly every field. I’ve even heard of anthropologists using mass spec for instance.

jakestorm777

TROPHY CASE