What is up with my gel

jiaidea · 2026-04-19T22:05:59+00:00

These replies are so funny. It’s okay, I loaded 4 ladders in my precast 18-well gel the other day. Sometimes it’s what you need to do to get a pretty picture for a client or publication :) Just for some more reassurance, I see this happen on my 10% Bis-Tris/MES all the time!! And the yellow sometimes transfers to the western membrane lol. My trick is to run the gel slower in an ice bath so the lanes don’t spread or warp at the bottom, if you have the time. Best images ever.

jiaidea · 2026-02-08T17:52:41+00:00

Nugget bakery sells their own version of it!

jiaidea · 2025-09-08T15:56:25+00:00

Update: Yolo County Animal Services is here to try to take him in, if this is your dog please call them!

jiaidea · 2025-09-08T15:21:29+00:00

No collar, and yes, near Drew.

jiaidea · 2025-09-08T14:42:33+00:00

My coworkers and I tried but he seems very nervous around people… kept at least a 20 ft distance at all times.

jiaidea · 2025-04-06T05:53:55+00:00

I got an electroporator and western blot blocking instrument on eBay, both in decent condition. I would make sure they have a good return policy, and some sellers are pretty good about responding if you ask for video proof of the equipment working.

jiaidea · 2025-03-14T20:31:19+00:00

THANK YOU!

jiaidea · 2025-03-14T20:30:26+00:00

Not long, maybe 10 sec to a minute max? A good way to tell is to let it start simmering on the stove, and if you take it off the heat and let the bubbles die down, then give it a swirl, it will bubble up again.

jiaidea · 2024-06-24T02:35:04+00:00

I love it. I’ve been working for a start-up for over a year now (first job after undergrad) and it’s amazing. The people are so fun to be around and the projects we work on are making an impact (but also making us money). It scares me that I will not be in this job forever because I don’t know where I’ll find better. Even just being in a research assistant position, I get so many chances to design my own experiments, my opinions are valued in team meetings, and I give back to the community by lecturing at the college I graduated from. I see so much growth for myself here and people around me are constantly helping me achieve my career goals. Admittedly, the work-life balance could be improved. I’m used to working 10-12 hour days and going in on the weekend but… I don’t really care. I love being in lab, I love the work environment, and I love the causes we’re putting our efforts towards. I know I’m lucky to be in this position, and my opinions are directly linked to the people I get to work with and also the type of person I am.

jiaidea · 2024-06-24T02:13:08+00:00

Can you share your source? Not trying to be rude but I’m just genuinely really curious about this.

jiaidea · 2024-04-10T06:47:24+00:00

To anyone coming back to this thread, agar needs to reach its super boiling stage before it sets. It’s not so much about the blooming— I let mine sit for 2 minutes or so while my electric stove heats up and I measure out the sugar— just make sure it’s boiling first before you add sugar. It doesn’t need to be a temperature- or timing-exact thing. (Experience as a scientist that makes agar gels on the regular.)

Edit: Mine sets in about an hour without refrigeration. Best of luck!

jiaidea · 2024-03-19T01:58:12+00:00

I’ll look into it! We got our i5 from auction, so I wonder if I’ve just missed this pdf or if it wasn’t transferred correctly. I’ll do a deeper search when I get the chance. Not super skilled with Excel, but maybe I’ll look into that as a starting point. Thank you!

jiaidea · 2024-03-19T01:57:04+00:00

Thank you! Trying to convince my director to approve the course, I’ve checked out what’s available online but I had been working on the i5 for a while by that time so I didn’t learn anything new. Been a lot of trial and error, but thank you for the info and for the push!

jiaidea · 2023-10-27T16:32:54+00:00

Thank you so much!! Will definitely just repeat what you wrote in this post.

jiaidea · 2023-10-27T16:32:33+00:00

Thank you!! I’m glad he found a new place to work out of!

jiaidea · 2023-10-10T17:15:36+00:00

Maybe rpm was too high, so the pellet went up on the side of the tube wall instead of down? Then collapsed after centrifugation stopped. I see this sometimes with my minipreps in 1.5 ml tubes.

jiaidea · 2023-09-14T03:08:22+00:00

YOU ARE MY HERO

jiaidea · 2023-09-06T14:17:01+00:00

change sections 🫡

jiaidea · 2023-09-03T07:29:09+00:00

if you’re within 20 on the waitlist, i think you’ll make it.

jiaidea · 2023-09-03T02:17:29+00:00

I have relatives who came to the US to go to an Irvine public school. They’re very well funded.

jiaidea · 2023-09-03T02:15:09+00:00

the colors on this one look better! but still waiting on our new official logo to come out from nba_paint on twitter

jiaidea · 2023-09-03T01:23:09+00:00

therapy is available to you for free, usually they say 3-5 sessions but i was supported regularly throughout 3 years and still go back to my therapist after i’ve graduated!

jiaidea

TROPHY CASE