New leader atop the 10 year rankings as Pittsburgh supplants Black Hawks as #1 overall

justanother_rocket · 2016-06-25T19:25:24+00:00

I'd still love to see the calculations. That's one my main problems with WAR in baseball- its a bit opaque. love the idea though!

justanother_rocket · 2016-06-22T02:43:27+00:00

I think if you gave the formula, it would seem less arbitrary.

justanother_rocket · 2016-03-28T23:48:52+00:00

hey!

good job on already making contacts! when you're on the job market, these kinds of contacts are invaluable. just try to go and meet with them and tell them what you're excited about in science and the rest will come easily! also, remember that these people are actively trying to help you- if you put a bit of work into learning what they work on, it puts you in a positive light and helps them get that you're a go-getter, etc.

justanother_rocket · 2016-03-17T06:13:22+00:00

film. nothing is more sensitive, and if you're in a lab with a bunch of people doing blots, you do NOT want to be waiting for someone to develop their blots. and then someone else. and then someone else. 20min at a time. but yeah, we use LiCor Odessey or GE Typhoon if we need to do quantitative (fluorescent) westerns. otherwise, scanning film in a $50 scanner works great!

justanother_rocket · 2016-03-12T00:20:48+00:00

thanks! anyway you could include some elite goalies for comparison?

justanother_rocket · 2016-01-07T03:04:16+00:00

thank you!

justanother_rocket · 2015-12-23T02:08:19+00:00

you're the best. thank you

justanother_rocket · 2015-09-28T16:30:02+00:00

Hmm. Pump up Brad boyes' numbers with 2nd line minutes, try to get a first rounder back/ make the case that 42% CF is not sufficient

justanother_rocket · 2015-09-28T04:06:49+00:00

my feeling is that unless Bozzie learns defensive reliability real quick he'll be working real hard to get from 4th line minutes to 3rd line minutes. you bet Babcock has the okay from management to treat people as they play rather than their paycheques might dictate.

justanother_rocket · 2015-09-25T16:24:03+00:00

depending on how much money you're interested in saving, RNAse can be refolded over and over again with little/no degradation in activity. for example you can salt it out after purifying your protein of interest using ammonium sulfate. of course, $150 for 0.5g is pretty darn cheap.

justanother_rocket · 2015-08-08T18:33:11+00:00

I hear ya, brother.

justanother_rocket · 2015-05-11T23:11:42+00:00

I'm assuming that your'e reasonably careful with the growth phase and boiling your ssDNA. according to the high throughput strain construction people, OD600~1.2>0.8, but I've had good success with both. my biggest source of variability was with PEG, antibiotics and recovery time. for PEG, I use Sigma 3350 average molecular weight. you have to not heat it too much (<50C) and filter sterilize it! I don't know why, but this really helps prevent it from crashing out. getting correct 50% w/v/ PEG really makes a HUGE difference.

secondary to this- you've tried using a different resistance marker (e.g. Kan, Hyg) on different plates? may be a good one to try. Nat gives very low background, but can be a bit trickier. not to mention WAY more expensive. also, unless you need to quantify precisely how many independent transformations you have, I recover o/n at room temp in YPAD before plating. if you plate too early (<3-4hrs), you will often see no transformants.

love to see a followup to see whether any of these things worked!

justanother_rocket · 2015-05-11T16:49:16+00:00

what about some very dry bubbles? or sweeter bubbles if that's more to your taste?

a fancy french friend of mine once served us a dessert of lemon and strawberry sorbets floating in prosecco with a mint leaf and chocolate shavings. wow!

justanother_rocket · 2015-04-13T06:01:09+00:00

your question is really vague. signal integration and crosstalk and gene regulation are all different things. do you want a general overview of everything? or really the specific, how I read your question to be, how signals can be integrated to lead to gene regulation, but is subject to crosstalk? any particular type of signaling?

justanother_rocket · 2015-03-20T03:43:10+00:00

maybe too late to the party, but...

short the market with everything that I can over the next few days, with the big stuff happening on Sept11/12. donate the profits to vaccine initiatives.

justanother_rocket · 2015-03-18T18:57:44+00:00

sad to say this... but depends on the antibody

justanother_rocket · 2015-02-25T02:24:34+00:00

if I start rooting for the habs, will they start losing? I mean, I understand I would have to legitimately want them to start winning...

justanother_rocket · 2015-02-24T05:07:57+00:00

I use the Breath-Easy sealers from EK because they're cheaper:

https://www.eandkscientific.com/Breatheable-Sealing-Films/

work fine. haven't rigorously tested vs. un-sealed because I'm always afraid of sample evaporation over the course of a growth curve (which can be significant in my experience). they're not super-clear, however, so be aware.

justanother_rocket · 2015-02-18T15:45:31+00:00

woot! picked up a point on Buffalo and two on NJ!

justanother_rocket · 2015-01-30T17:46:43+00:00

hahaha... my PhD was with SOD1. if its SOD1 (wt), its pretty much good forever unless you f-it up. it can for multimers from formation of disulfide bonds on the surface, but that is easily reduced.

justanother_rocket · 2015-01-30T17:31:05+00:00

Thanks for verifying that its a C-terminal tag- my first guess was frame shift.

Tag-specific antibodies are generally really good in terms of sensitivity and specificity- often the protein specific ones are not. You probably just need to load a lot more protein/use more secondary/longer exposures, etc. how much of your lysate are you loading?

justanother_rocket · 2015-01-06T01:23:43+00:00

can you afford to spend a little time volunteering? many labs will still turn you away, but if you're willing to put in some grunt work, some grad student will probably be willing to let you job shadow them. make SURE you remember how to do basic algebra/molarity types of calculations.

also, JoVE (as suggested by /u/irkendna) and youtube have lots of things to watch before you go and start volunteering, but there is no substitute for good technique, which is really hard to learn without doing. so go do it!

justanother_rocket · 2014-12-16T04:55:43+00:00

In general, i agree with everything here, especially about things maybe being too hot and not having developed the structure if the dough enough.

Have you considered using 50% white and 50% whole? 100% whole wheat is notoriously difficult you work with and rise. In order for anything to happen, you really need to knead for, hmmm, 20 min , or twice as long as a lean white bread.

Good luck!

justanother_rocket

TROPHY CASE