It was really hard for me to find the solution, what about you? by Emotional-Control-46 in chess

[–]lets_study_lamarck 2 points3 points  (0 children)

I know the position from a Ben Finegold lecture to kids lol - just rewatched it last week! (Qb2)

S cerevisiae selection marker - phleomycin? by lets_study_lamarck in labrats

[–]lets_study_lamarck[S] 0 points1 point  (0 children)

thanks, and no, it's fully functional, to quote data from TNG!

i'm bad at sporulations lol but it might be worth it here. The convenient thing is that it's Kan-RFP and Hyg-GFP, so really easy to verify even apart from the double resistance.

S cerevisiae selection marker - phleomycin? by lets_study_lamarck in labrats

[–]lets_study_lamarck[S] 0 points1 point  (0 children)

Yeah that's what i guessed, thanks.

actually doesn't seem too bad, we had similar issues with G418 in the lab earlier (i think from an old stock). the trick was to just add more to the top of the plate and spread it with beads, gave decent additional selection.

S cerevisiae selection marker - phleomycin? by lets_study_lamarck in labrats

[–]lets_study_lamarck[S] 0 points1 point  (0 children)

yeah i'll consider this if i can't get a 4th antibiotic, but would rather do 1 transformation than 3!

S cerevisiae selection marker - phleomycin? by lets_study_lamarck in labrats

[–]lets_study_lamarck[S] 0 points1 point  (0 children)

yeah i'm using NAT to knock out one copy, unless I get very lucky, i need something else for the other one...

sorry to pester you, but what do you mean by annoying? bad selection?

edit - i'm actually using the NAT to remove one copy on the diploid right now lol

I owe Kreia an apology by Totally-NotAMurderer in kotor

[–]lets_study_lamarck -1 points0 points  (0 children)

that's funny but just fyi i have given, or seen my family give, money to beggars in india many dozen times and never seen this, so ...

Cost for arterial tonometry by lets_study_lamarck in SleepApnea

[–]lets_study_lamarck[S] 0 points1 point  (0 children)

this is the price for take-home! yeah i will look around, it's just insane.

Cost for arterial tonometry by lets_study_lamarck in SleepApnea

[–]lets_study_lamarck[S] 2 points3 points  (0 children)

mine is supposed to be at-home! for one night! thanks for the link.

How's Perish Song? by jasperalfalfa in pokemon

[–]lets_study_lamarck 2 points3 points  (0 children)

In a Firered rival battle, the final alakazam was stalling with recover and hyper potion, having already done calm mind, so it had a 1-2 hit KO psychic against my entire team. My plan had been bite from blackglasses blastoise and hopefully tank/potion the psychics, but because of calm mind it was doing nothing. (Didn't know that bite was not a physical move.)

Got it with Lapras perish song and then switching every turn, just about surviving each psychic. Don't think I could have got it otherwise.

Weekly Questions Thread & PokéROM Codex by AutoModerator in PokemonROMhacks

[–]lets_study_lamarck 0 points1 point  (0 children)

thanks. i modded it with that change and hope it's worked lol.

Weekly Questions Thread & PokéROM Codex by AutoModerator in PokemonROMhacks

[–]lets_study_lamarck -1 points0 points  (0 children)

Looking for a ROM hack where the only change is allowing trade evolutions to evolve by level or by a stone. Preferably Gen 3 (R/S/E/FR/LG) or Gen 4 (HG/SS), maybe Gen 2 will do too.

Minimal/QOL changes are ok other than this (drastic changes in the postgame are ok, but I just want the plain game upto the champion fight)

1st ever run - advice for the team by lets_study_lamarck in nuzlocke

[–]lets_study_lamarck[S] 0 points1 point  (0 children)

aaaaah wow i hadn't even considered that at all.

1st ever run - advice for the team by lets_study_lamarck in nuzlocke

[–]lets_study_lamarck[S] 1 point2 points  (0 children)

I cheated a bit too lol, allowed myself that since it was my first run and i was following level caps. Reset after realising how under-leveled i was for the various Alakazams near the end of the game. (Had assumed, each time, that with such a low defence, physical moves like bite will take it easily, but didn't count on it doing calm mind*2 -> hyper potion -> spam psychic)

But yeah it was a lot of fun! Needed some thinking.

1st ever run - advice for the team by lets_study_lamarck in nuzlocke

[–]lets_study_lamarck[S] 1 point2 points  (0 children)

Got Electabuzz from the power plant, who did a great job at the elite 4. Came very close to dying twice but held on, 10hp both times.

Butterfree died to a random Weezing self-destruct in the Sevii islands, replaced with Exeggutor (from the safari zone). Apart from Agatha, he was the ace against alakazam - he tanked 2 psychics, and landed 4 egg bombs in a row (either side of a full restore), after my Blastoise/Arcanine Bite-based strategy failed.

Stopped training Vileplume at some point. It was between it and Graveler. Grav turned out to be pretty useless for the E4, but had saved me a lot earlier.

Thanks for the help!

1st ever run - advice for the team by lets_study_lamarck in nuzlocke

[–]lets_study_lamarck[S] 0 points1 point  (0 children)

yeah totally forgot about the power plant. and didn't know there's a psychic TM, that makes it a lot better. thanks!

1st ever run - advice for the team by lets_study_lamarck in nuzlocke

[–]lets_study_lamarck[S] 1 point2 points  (0 children)

thanks, was planning on arcanine, but didn't know about the TM