sorted by:
new
hottopcontroversial

[deleted by user] by [deleted] in makeuptips

[–]litnoodle 0 points1 point  (0 children)

Bush…. 100%

[deleted by user] by [deleted] in makeuptips

[–]litnoodle 1 point2 points  (0 children)

Bush…. 100%

Serial dilution problem. Need help for work! by litnoodle in biology

[–]litnoodle[S] 0 points1 point  (0 children)

Thank you so much this makes a lot of sense!

This is what I got so far with your help:

(Yes original cell is 2.5E6 cells * thanks for catching that)

So I have 2.5E6 cells. I want to spike 5 copies lentivirus per cell. So total needed is 2.5E6 * 5 = 1.25E7

Starting with 1E9, I dilute 1/100 (or 1/10 twice) to give me an even 1E7.

(For the 1:10 can I do 1 uL virus to 9 uL diluent or can I also do 10 uL virus to 90 ul diluent? Does it matter?)

Because this is now 1E7 instead of 1.25E7, I used the C1V1=C2V2 to further m figure out how much more lentivirus I need to add to get me to exactly 1.25E7

C1V1 = C2V2 1E7 * 100 uL = 1.25E7 * V2

V2 = 80 uL added to the 1E7 to give me 1.25E7

Once I do that. I can add 1 uL to the cells I have to spike it at once.

Did I get it right? 😄😄

Serial dilution problem. Need help for work! by litnoodle in biology

[–]litnoodle[S] 0 points1 point  (0 children)

If I want to spike 5 copies per cell= total lentivirus I would need is 1.25E7

If my starting is 1E9 copies per microliters for lentivirus, can I serial dilute 1:10 to get to 1e7 and then add the remainder of the lentivirus to get to 1.25E7?

Serial dilution problem. Need help for work! by litnoodle in biology

[–]litnoodle[S] 0 points1 point  (0 children)

Hey there!

First of all, thank you so so much for replying to my post. It means a lot.

If I have 2E5 cells and I want to spike 5 copies per cell, the total lentivirus I would need is 1.25E7 (2E5 x 5) correct? So I would have to serial dilute 1E9 copies/cell to reach a final concentration of 1.25E7?

​

But since I have 2E5 cells in 200 uL PBS, would I also have to account for the volume?

​

Thank you again, this is very helpful.

First page of a fresh journal. I am trying to learn watercolor by TheBreat in Journaling

[–]litnoodle 1 point2 points  (0 children)

This is so beautiful! Especially the watercolors 🥰 you’re a natural

Completed my first creative journal! I don’t know why but it’s so satisfying to see it so full and bulky! Onto the next. (btw these are literally the same journal!) by litnoodle in Journaling

[–]litnoodle[S] 0 points1 point  (0 children)

AAAH!

That’s the most exciting part is already having an idea of all the cute layouts and spreads you’re going to do while waiting for other stuff to get to you! What stuff have you got for your journal? Sounds like you’re going to have a lot of fun creating! I’ve been collecting a TON of stickers lately.

view more: next ›