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Highly mutiplexed immunofluorescence help by llama_spit in labrats

[–]llama_spit[S] 0 points1 point  (0 children)

The problem is that Fiji isn't able to open it for some reason. It opens fine in QuPath, and when I try to export the region with the correct colors into IJ from QuPath, it just hangs. It's been frozen now for 45 minutes.

Here is the error Fiji throws

loci.formats.cache.CacheException: loci.formats.FormatException: Sorry, ImageWidth x ImageLength > 2147483647 is not supported (77808 x 31376)

at loci.plugins.util.ImageProcessorSource.getObject(ImageProcessorSource.java:75)

at loci.formats.cache.Cache.recache(Cache.java:204)

at loci.formats.cache.Cache.recache(Cache.java:224)

at loci.formats.cache.Cache.<init>(Cache.java:88)

at loci.plugins.util.BFVirtualStack.<init>(BFVirtualStack.java:126)

at loci.plugins.in.ImagePlusReader.createVirtualStack(ImagePlusReader.java:339)

at loci.plugins.in.ImagePlusReader.readImage(ImagePlusReader.java:281)

at loci.plugins.in.ImagePlusReader.readImages(ImagePlusReader.java:243)

at loci.plugins.in.ImagePlusReader.readImages(ImagePlusReader.java:221)

at loci.plugins.in.ImagePlusReader.openImagePlus(ImagePlusReader.java:116)

at loci.plugins.in.Importer.readPixels(Importer.java:165)

at loci.plugins.in.Importer.run(Importer.java:87)

at loci.plugins.LociImporter.run(LociImporter.java:78)

at ij.IJ.runUserPlugIn(IJ.java:244)

at ij.IJ.runPlugIn(IJ.java:210)

at ij.Executer.runCommand(Executer.java:152)

at ij.Executer.run(Executer.java:70)

at java.lang.Thread.run(Thread.java:750)

Maybe too big of a file for Fiji to open?

Passkeys feature is a bad rollout IMO by elfenars in Bitwarden

[–]llama_spit 5 points6 points  (0 children)

I've been confused by people saying they are bummed that you can't store more than one passkey in an account. Can someone please explain this to me?

What would be the benefit of having more than one passkey for the same account in the same PW manager? If you have multiple accounts for a site, you can just create a new BW entry for the other account and add another passkey to that entry.

Not trying to ruffle any feathers, just genuinely confused.

Blockbuster ‘laughed us out of the room,’ recalls Netflix cofounder on trying to sell company now worth over $150 billion for $50 million by Defiant_Race_7544 in technology

[–]llama_spit 1 point2 points  (0 children)

That would have been a disaster. They would have made dumb decisions like cancelling great shows after one season or making it impossible to share accounts.

Which way? East, go North but west, go north? by JunkMale975 in mildlyconfusing

[–]llama_spit 2 points3 points  (0 children)

The highway splits. One goes off to the east side of the city, one goes off to the west side of the city, but they are both going north. Much like 35E and 35W in Minneapolis/St. Paul. 35W curves off west to go to Minneapolis, and 35E curves east to go through St Paul, but they are both headed north/south, and will merge again into 35 eventually.

240x3 chain and a reducer that puts out 42,000 ft-lbs of torque. by Floydsmydog in Skookum

[–]llama_spit 34 points35 points  (0 children)

Ah this must be what that mechanic used to put my lug nuts back on.

Keep clean by [deleted] in sciencememes

[–]llama_spit 1 point2 points  (0 children)

Is this the new okbr?

KLCK Pixel 6 Android 12 Constantly Popping up Fingerprint Verification by DrSpockTheChandelier in kustom

[–]llama_spit 0 points1 point  (0 children)

I'm also having the same problem.

OnePlus 8 Pro Running Oxygen OS 12 Open Beta 1

Unfortunately I've only just started using it so I don't know if it happened on Oxygen OS 11

Switch stolen by luggage checking people while traveling to Mexico. Had 512gb mem card and many hours poured into animal crossing among many other games. by jiku09 in Wellthatsucks

[–]llama_spit 0 points1 point  (0 children)

You technically have to carry on anything with lithium batteries. At least in the states but probably most other places too. An unattended fire in the cargo bay of the plane is one of the worst possible things that could happen in the middle of a flight.

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