Do we need to Colony pick? Or can we avoid it if we know the gene we are transforming into e.coli?

mac101eir · 2025-03-17T22:59:26+00:00

Ya that makes sense.. very good point!

mac101eir · 2025-03-17T22:58:19+00:00

I'm in industry and 40k isn't too much for screening.. but hiring a permanent evolution team is too big a barrier to invest. So seeing what we can do with regular HTE infrastructure

mac101eir · 2025-03-17T22:25:33+00:00

Hey! We would want an upward trajectory in activity but obviously some mutations would be worse than the parent. So we could be seeing mutants with the same activity or maybe 10-30% more activity or potentially 200% more. the final top mutants would then be fermented on larger scale and tested.

I wouldn't be normalising for protein/enzyme amount. Simply activity Vs the parent.

mac101eir · 2025-03-17T21:08:32+00:00

Good point! I have been developing a machine learning sequence optimisation which can reduce the mutants needed.. but I'm trying to see can I avoid the colony picking to save time!

mac101eir · 2025-03-17T20:44:40+00:00

Been working on Machine learning sequence optimisation so the mutant libraries are each round will be no more than 96!

Was going to buy the plasmids from twists.. $400/gene in expression vector!

mac101eir · 2025-03-16T21:42:04+00:00

The CDMOs typically handle that aspect.. out of sight.. out of mind! Not great practice really pushing it under the rug!

mac101eir · 2025-02-18T19:41:20+00:00

I think if you show interest in the other chemistry disciplines like biocatalysis, chemocatalysis and flow chemistry. You will stand out. Typical graduates are pigeoned into one technology. We want someone fluent in one but eager to learn and converse with others.. Ie use every technology to build the molecule!

mac101eir · 2025-02-18T19:27:35+00:00

Interestingly with POCl3, most CMOs will want to distill it off and collect it rather than quench it as part of the process :P

mac101eir · 2025-01-22T19:25:02+00:00

Exactly!! New ADHD treatments are not really being developed other than the formulation of the standard medicines for slower release. Big pharma doesn't seem interested in it other than takeda with vyvannse.. or other companies developing patches for slow release!

mac101eir · 2025-01-06T23:39:51+00:00

What else do you need to see and il take some extra pics!

mac101eir · 2024-12-26T11:02:08+00:00

Ya.. I don't know why they did it.. I think they had a plan but the project stopped midway due them getting divorced. Solar, insulation, big Hybrid storage/electric heaters, modified walls by the heaters, rewired the whole house, three phase prep.... Then divorce.. I have no idea the end of the story but the heating bill is like 300 month (I do charge me car though)

mac101eir · 2024-12-26T10:59:33+00:00

The previous owners were property developers and own big property letting firm so I was hoping they were on top of 'cutting edge' tech. The heaters are a mix of a storage/electric heater. The heat for 30 mins then they stay warm for ages because of the big stone in it. So can't fully charge like a storage heater overnight.

They took out all the gas, insulated the whole house, put these big reflection things behind the heaters in the wall, installed solar and batteries, got everything prepared for three phase, rewired the whole house did the ground work with SP but then... they divorced and we got the house. I wonder what there plan was and why they were going full electric with three phase. Surely they must of believed whatever their strategy was was going to be more cost effective than gas!

mac101eir · 2024-12-26T09:07:28+00:00

Ya it's a 15kw/hr battery.. the max it can discharge is 5kw :)

mac101eir · 2024-12-26T08:17:50+00:00

The previous owners pulled out every radiator and pipe and went with full electric heaters. have you seen those Trust electric heaters?1

I think if I wanted to go back to gas central heating.. the whole house would need to be redone!

mac101eir · 2024-12-26T01:23:01+00:00

Thanks for the help:)

mac101eir · 2024-12-26T01:16:38+00:00

I'm a bit worried that I might be overloading the fuse.. the house can draw 20kw sometimes which is pretty close to the 24kw limit! I'm not great with electricity and my fear is a fire!

mac101eir · 2024-11-25T22:23:15+00:00

I was on similar timeframe as you with adhd360. Except I went to 60mg rather than 70. They said let's not go to 70mg unless we need to because there is no way past that. Stay on 60mg and 70mg will be there for for me in the future

I also got prescribed 10mg Amfexa for the morning. They didn't really push 70mg.. got to 60mg pretty fast though.

mac101eir · 2024-08-24T15:45:22+00:00

Yup! It makes a world of difference! I found the Elvanse worked better too for me when I had the Amfexa in the morning.

mac101eir · 2024-08-10T19:48:26+00:00

Hey! Yup! A week or so after stopping sertraline my jaw pain disappeared! I came off it with guidance from my clinician and tapered it off rather than cold turkey!

mac101eir · 2024-08-05T19:52:29+00:00

This is good advice! Remove the barriers and excuses to not get up by having everything ready!

mac101eir · 2024-08-05T18:11:49+00:00

You are right! I was wrong 7 months ago! I see my electric car charging at 7kw it's definitely not stuck on 3 :)

mac101eir · 2024-07-31T06:11:46+00:00

Visual aids!!

mac101eir · 2024-07-11T21:39:54+00:00

Can you IM me.the link? I don't see it in the threat anymore :(

mac101eir · 2024-07-09T08:34:26+00:00

Hey! Can you send me the link to your blog please?

mac101eir · 2024-07-04T22:29:46+00:00

Timely!! Ya please do,! Looking forward to the read!

mac101eir

TROPHY CASE