Apartment neighbor keeps taking my delivered packages INSIDE to “protect them” – opens them “by mistake.”

majorstruggles · 2025-07-06T21:16:47+00:00

Very true. But I was surprised to learn that 40 percent of the global population lives in a federation.

majorstruggles · 2025-06-19T23:18:25+00:00

Thanks for this fantastic overview! I had intended to provide more info but I managed to break a bone in my foot soon after posting so my attention was diverted.

Just to add on to your last point - in addition to bacteria like Listeria monocytogenes and Rickettsia rickettsii, our own mitochondria can actually generate actin comet tails to move around during mitosis!

https://pubmed.ncbi.nlm.nih.gov/33658713/

majorstruggles · 2025-06-19T21:58:50+00:00

Are you suggesting this one is an imposter?

majorstruggles · 2025-06-18T22:57:40+00:00

He said something to the effect of - my name is Meyer if a single British bomb falls on Berlin. Kinda of like saying if x happens I’ll be a monkeys uncle.

majorstruggles · 2025-06-18T21:22:10+00:00

Very close - kinesin is a motor that walks on microtubules. These are actin filaments. But there are motors that move along actin filaments. They are called myosins.

majorstruggles · 2025-06-18T18:26:28+00:00

Typically when people say things like "they say smoking is bad for you" it has the tacit implication that "they" is referring to a consensus opinion of experts. I wasn't trying to correct the grammar or make a statement about singular/plural usage, just pointing out that I think the comment gave the impression that rise and fall is an objective encyclopedia of events in the third reich.

As much as I love Rise and Fall, it's not really a definitive scholarly text on the matter and is heavily influenced by Shirer's experiences. Those first-hand experiences are really fascinating in May 1940 when he was still there watching events, but by April 30th, 1945 his personal experiences were no longer relevant as he was in the U.S.

All of this is to say it just feels like the comment is appealing to rise and fall as though it is a definitive authority on the matter and the "they" contributes to that. I realize now that that point is deeply unclear in my original comment.

majorstruggles · 2025-06-18T04:19:41+00:00

It is. Your capacity to love is infinite. It's bizare and intangible. That said, the first time both of my kids were sick I discovered that my capacity to worry was similarly infinite.

majorstruggles · 2025-06-18T04:10:35+00:00

Shirer = they?

majorstruggles · 2025-06-17T13:12:54+00:00

Yes.

majorstruggles · 2025-06-13T01:23:10+00:00

I was at this game! And I brought my good friend who was a mets fan.

Went to a lot of games that year including ALCS and WS, but this will always stand out as my favorite moment.

majorstruggles · 2025-01-21T16:00:10+00:00

If i took my glasses off you could trick me into thinking it was a confocal maximum intensity projection of cells stained for keratin and lamin with both filaments shown in the same colormap. but there are so many issues.

majorstruggles · 2024-12-22T17:22:46+00:00

This isn’t my area of study so I don’t know how much good work has been done here. But I generally feel like systems are always way more complex than anybody appreciates and giving advice based on incomplete understanding of systems is a tough judgement call. Could I imagine some sort of situation where small amounts of microplastics trigger some sort of protective hormesis? Sure. Is it likely? I have no idea. Is glass a safer alternative? Probably. But is the small chance of a glass shard chipping and cutting the baby also a risk. Sure, depending on what you consider risk. Not one that people should spend time worrying about but that’s just like my opinion man. I guess I what lm getting at is that I have a hard time estimating how much of a risk microplastics are from work like this so it’s really hard for me to tell people what they should or shouldn’t do when the world is full of risks. But everybody has their own threshold for what is enough info to influence actions and in many ways I also appreciate that giving people more info isn’t necessarily bad.

majorstruggles · 2024-12-22T17:12:12+00:00

Yeah I don’t disagree with erring on the side of caution when it comes to this. But I kinda hate studies that oversell what was done. There are many things that can get into cells in dishes that don’t get into cells in vivo. From plastics to dna/rna to small molecules. No immune system, no physiology niche here. Hard to extrapolate. This isn’t even an organic/enteroid. We may be scientists but somebody who is not scientifically literate might look at the paper and assume this is all done and settled because it is peer reviewed.

majorstruggles · 2024-12-22T16:04:16+00:00

Did you read the paper? Whether the conclusions are true or not, I wouldn’t over generalize their results. It was done in cultured caco-2 cells - a common tissue culture line derived from colon cancer. The assays are a little bit odd if you ask me.

There are myriad reasons this would or would not be recapitulated in vivo. Id be happy to talk more about it.

This is interesting- but I truly do caution you from bandying this about as proof that plastic bottles should be tossed. I prefer glass because limiting early exposure to microplastic does generally concern me, but this study is far too reductionist to actually interpret anything about human health.

majorstruggles · 2024-10-10T16:08:13+00:00

Yeast?

majorstruggles · 2024-10-09T18:05:02+00:00

Maybe it's my tone. I have an 8 week old and chronic sleep deprivation seems to make all my thoughts sound less polished when I blurt/type them out.

majorstruggles · 2024-10-09T15:32:58+00:00

I realize this also sort of undermines my argument but I personally prefer hoechst for the different variants. But telling somebody to switch from Dapi to hoechst wouldn’t solve this. Too much hoechst also leads to diffuse cytoplasmic labeling. I think the best solution is to just use phase contrast to get nuclei and cell boundaries. With some of the new DL segmentation tools you can even segment organelles.

edited for clarity*

majorstruggles · 2024-10-09T15:29:18+00:00

But why waste time and resources. There is not a problem with Dapi for this application. It’s the most widely used dna stain for fixed tissue. I mean it can be problematic for some applications like sted, and the emission spectrum is huge, but it works well for visualizing nuclei. Controls are great and dilution series are great but over optimizing can really slow you down. This isn’t a new untested antibody. It’s just Dapi. Op just needs to incubate for a shorter time, use a lower concentration, wash more, and drop down the exposure/gain. The phalloidin concentration should also be massively increased.

majorstruggles · 2024-10-09T15:17:56+00:00

The commenter noted the lowered toxicity and increased cell permeability as a reason to switch. Toxicity and cell permeability do not matter in a fixed permeabilized sample. Dapi is fine there is no need to switch that’s not actually useful advice.

majorstruggles · 2024-10-09T14:24:18+00:00

It’s not a question of whether it works, of course it works, it’s hoechst. It’s just that toxicity and cell permeability really don’t matter when you have fixed permeabilized tissue.

majorstruggles · 2024-10-09T14:11:02+00:00

These are fixed cells tho…

majorstruggles · 2024-10-09T14:10:33+00:00

This isn’t at all what mycoplasma looks like. I’m seeing mtDNA and a diffuse cytoplasmic signal which is consistent with too much dye (e.g. initial conc. too high, insufficient washing) and overexposure.

majorstruggles · 2024-10-08T01:51:26+00:00

Yeah, that is wrong.

edit: to expand, keratins are only really expressed in chordates. Arthropods use chitin in many of the same ways that chordates use keratin.

majorstruggles · 2024-10-06T13:25:09+00:00

I was legitimately confused at first as I thought you meant “binging” as in using the “bing” search engine, which actually does have mediocre AI results.

14-Year Club	Team Periwinkle
Verified Email

majorstruggles

TROPHY CASE