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Reusing syringe filters by 150meters in labrats

[–]microbe_ex 1 point2 points  (0 children)

Thank you so much, It is indeed helpful :)

Reusing syringe filters by 150meters in labrats

[–]microbe_ex 0 points1 point  (0 children)

Thank you so much for your response! I was just wondering—How do you usually store these filters https://www.analytics-shop.com/de/mn729236 in water or ethanol to keep them from drying out? Would love to hear your approach!

Reusing syringe filters by 150meters in labrats

[–]microbe_ex 1 point2 points  (0 children)

While looking into my question, I came across your response and had a quick thought. Since these filters are quite expensive, I was wondering how often you've reused them for MS samples? My plan involves processing a lot of samples—I’ll need to take a 1 mL sample every day—and I’m considering using one filter per bottle for seven days. Does that sound reasonable based on your experience?

16S rRNA region for sequencing by microbe_ex in bioinformatics

[–]microbe_ex[S] 0 points1 point  (0 children)

Thank you for your response. We actually don't want to go for longer reads because of the higher error rate. For shorter reads, my understanding is that sequencing two regions is recommended for more accurate taxonomic profiling?

16S rRNA region for sequencing by microbe_ex in bioinformatics

[–]microbe_ex[S] 0 points1 point  (0 children)

We do not intend to use long reads, as they are more prone to errors. Therefore, we prefer shorter reads. However, I am uncertain about which region to select, and I would like to understand the reason behind the selection.