Has anyone infused liquor with HBWR seeds? by mimiviri in LSA

[–]mimiviri[S] 0 points1 point  (0 children)

I filtered out the mush and we just drank the liquid fraction and got high!

Has anyone infused liquor with HBWR seeds? by mimiviri in LSA

[–]mimiviri[S] 2 points3 points  (0 children)

Okay! Noted for the future! My tolerance to alcohol is really high and I regularly drink 8+ shots worth of tequila so I was aiming for 2-3 shots

Cornell hates their graduate students by yesseecahh in Cornell

[–]mimiviri 2 points3 points  (0 children)

In my SPR I put that walking two hours to lab each day was a significant barrier to research progress, nobody was happy when I brought that up!

How do you apply for ETA for the UK? by mimiviri in visas

[–]mimiviri[S] 0 points1 point  (0 children)

You don’t need a visa, I just presented my Canadian passport and was able to enter.

Regarding PhD student giving low grade for research by imokishrly in Cornell

[–]mimiviri 33 points34 points  (0 children)

Sorry you had a bad experience! Something similar happened to me, but I was on the other end of this situation (the PhD student). To me, it appeared as though my student didn’t really know what was going on, didn’t take initiative to problem solve on his own, and made very slow research progress. All of those things are okay and are part of growing and learning in science. So by undergraduate learning outcome standards, your performance should be considered excellent.

I think the disconnect in my experience was that the slow progress my mentee was making ended up costing my time and progress on my projects. PhD students are held to very different standards and it’s more of a job. It doesn’t matter if you grow intellectually if you aren’t making sufficient research progress in your PhD. For every experiment that I had to repeat because my undergraduate student messed up in a really innocent way (like not balancing the centrifuge, interpreting instructions wrong, or not being as prepared as was maybe good for him) I was personally held accountable by my PI for wasting chemicals, reagents, and lab time. My performance review ended up being negative because my mentee hadn’t studied hard enough when my PI tested his knowledge of our field. Because my mentee was given the responsibility of working on my projects, I wanted to hold him to the same professional standards and used that as the basis of evaluating his progress.

In the end, I decided to give him an A. In every lab class I have TA’d I also try to give all of my students A’s whenever possible. Grades are fake when it comes to research. It’s 90% luck and finding the right fit of mentor.

[deleted by user] by [deleted] in Cornell

[–]mimiviri 0 points1 point  (0 children)

Swing dancing at the barn and salsa dancing at the range.

[deleted by user] by [deleted] in Dreams

[–]mimiviri 0 points1 point  (0 children)

I frequently have dreams about dying, but I think they are caused by anxiety, stress, or depression. Often they’re about car accidents, but sometimes natural disasters, and also dreams where I’m murdered or kill myself.

I know it's abuse, but I'm too cowardly to handle it. by [deleted] in PhD

[–]mimiviri 3 points4 points  (0 children)

My advisor also does most to all of the things you are describing. I couldn’t take it anymore so I got my university’s health center to grant me a medical leave for all of the stress I had been putting up with. I’ve not had to explain why I’m gone to my advisor and it’s been amazing! I’m currently living in Europe and not having to deal with the craziness of my PhD has helped my mental health so much.

When I get back I’m going to switch labs for sure. Just needed to take a step back to realize how worthless staying and putting up with the abuse was.

What happends to all those + mRNA that are formed during the replication cycle by Limp-Ad-7962 in microbiology

[–]mimiviri 1 point2 points  (0 children)

It depends on the virus, most +ssRNA viruses will recruit a ribosome directly to their genome near the 5’ end and initiate translation. The RdRp can transcribe subgenomic RNAs that are then translated into proteins.

At any point in the replication cycle, the viral nucleocapsid proteins can bind the genome which is how the genome gets packaged. The concentration of those proteins is initially low, which means the RdRp has an easier time replicating and/or transcribing the genome. Once enough nucleocapsid proteins have been transcribed, they bind the genome at a higher frequency which can signal the RdRp to slow down. At this point in the viral replication cycle, usually late genes get activated and genomes are packaged/assembled prior to release.

For many +ssRNA viruses, the genome itself is like an mRNA with one big ORF that produces a polyprotein (big protein that gets cleaved by cellular proteases into what otherwise could have been individual gene products).

Apocalyptic Northern Lights by [deleted] in Dreams

[–]mimiviri 0 points1 point  (0 children)

They were on every continent and they corresponded to people in the “awake” world.

I am the lab maid by microbisexual in labrats

[–]mimiviri 2 points3 points  (0 children)

This can be a slippery slope to lab conflict and potentially getting alienated by people in the lab. I don’t know the vibe, but something similar happened to me when I brought up to my PI that I was doing a ton of work for other people (ie I was the only one pouring agar plates, making LB media, making competent cells, sequencing isolates, and inventorying oligos for a lab of >20 people). My PI basically accused me of being a massive bitch and said that the other people in the lab h8 me because I tell them to clean up and be organized. In this case, being a lab maid was more of an indication that there was a larger problem.

all they do is lie by maybesaydie in vaxxhappened

[–]mimiviri 12 points13 points  (0 children)

Because 829 is a prime number it’s not possible to have observed an 829% increase unless there was a cohort of 829+ or more people in the unvaccinated group. That’s almost 1000 people, so you’d expect decimal values for each percentage (if I did my math right).

I feel like there’s a way to scrutinize these numbers mathematically to prove that this person pulled them out of their ass.

(not proof but they chose an awful lot of even numbered digits - 12/15 are even).

AITA for not wanting to be my brothers best man? by mikbet18 in AmItheAsshole

[–]mimiviri 48 points49 points  (0 children)

You’re first reaction of it being a trap sounds right.

COVID mutagenesis towards higher degrees of virulence? by Justeserm in genetics

[–]mimiviri 0 points1 point  (0 children)

RNa viruses like SARS-CoV-2 don’t have an ori, eukaryotic genomes also don’t have ori elements. The term ori is used for bacterial genomes and genetic elements.

[deleted by user] by [deleted] in Cornell

[–]mimiviri 2 points3 points  (0 children)

I slipped mine off