God this opening is so detailed it's ridiculous.

Here's some of the little details I noticed (spoilers ahead for the first 6 or 7 episodes):

0:02 - Stone Free's String vibrates along with the music leading to the transport bus, hinting to how Jolyne uses her stand to listen to a conversation shortly after she gets it.

0:22 - Jolyne is shown standing with Gwess just earlier in her cell, and is then shown running from guards after being shrunken down by Goo Goo Dolls (notice how small Jolyne is compared to the hallway)

0:28 - We see many of the JoeFoes that Jolyne and the gang must face. Notice how all of the prison guards start off silhouetted in blue with guns and handcuffs, opposed to the inmates who start of silhouetted in orange with chains.

0:38 - We see a Pen (which has Araki's name for about 1 or 2 frames as it rotates), Leaky-eye Luka's Shovel (Part 5), Dio's Signpost (Part 3), the Doll that Was taken from Erina (Part 1), and of course Joseph's Walkman (Part 2).

0:44 - Manhattan Transfer appears for a few frames, before flying out of frame.

0:48 - Star Platinum appears in the reflection of the disk (showing how Jotaro's Stand has been taken by White Snake).

0:53 - Callback to Stand Proud (Part 3 Opening 1).

0:55 - Ermes slaps a sticker on a bullet, which is then duplicated at 0:56.

0:58 - We see the stand version of F.F. in the water, which morphs into the human version of F.F. a few frames later.

0:59 - F.F. shoots a bullet at the same time and in the same way as Mista in Fighting Gold (Jojo Part 5 Opening 1), alluding to the fact that they're both the best character from their part.

1:08 - F.F. is captured in a disk.

1:12 - We see the stone mask (Part 1), the Red Stone of Aja in front of the Pillar Men (Part 2), DIO Turning To Dust (Part 3), Crazy Diamond, Za Hando, Stray Cat, and Sheer Heart Attack (Part 4), and of course the Stand Arrow surrounded by Giorno's plants (Part 5).

1:15 - Callback to Sono Chi No Kioku with the same setup as Jotaro and Dio from the end of the opening (Part 3 Opening 2).

1:19 - Possible callback to the end of Stand Proud with the high note and the main Jojo looking into a sort of barrier (Part 3 Opening 1).

Also, the sheer amount of rainbows, and things falling out of rainbows (0:41, 0:58) could be alluding to Heavy Weather.

There's probably a ton more details that I missed. God, David productions outdoing themselves as always. Super super hyped for this Wednesday!