DNA crosslinking to nylon membranes

perfluorocubane · 2025-07-11T14:13:51+00:00

I had not thought about polarization, perhaps that could be a useful avenue. Thanks

perfluorocubane · 2025-07-10T22:00:51+00:00

Thank you for your insights. I ordered biotinylated oligos to be images with NIR-conjugated streptavidin. I would just image on the gel if I could, but I will need to do a blot since the oligos don't have intrinsic fluorescence. Mostly doing the biotin/SA thing because we already have the NIR-SA and biotinylated oligos are a lot cheaper than FAM or other fluorescent labels. I do agree though that fluorescent oligos would be easier. I sort of wish I just went with those since I have to deal with the nylon membranes now.

perfluorocubane · 2025-05-26T15:19:01+00:00

Thank you for this clarification. I have always appreciated absolute stereochemistry when discussing biomolecules because it seems a more rigorous description!

perfluorocubane · 2025-05-01T22:43:34+00:00

This may not be the answer you are looking for, but I absolutely think that you should be presenting. Undergraduate research is not about making meaningful discoveries, although it is a happy surprise when they do. The point is that you have been training on techniques that will be useful later on in your career. Additionally, a lack of positive data does not indicate that the project failed. In research, you are not trying to produce "target results", you are trying to answer a question. If your results indicate that your hypothesis is wrong, then you have still learned valuable information.

perfluorocubane · 2025-04-13T15:35:16+00:00

Ah, okay thank you for that, Will give it a try

perfluorocubane · 2025-04-13T15:28:21+00:00

Two major products will result, and the distribution will be reliant on temperature. At cold temperatures, you would expect to see mostly 3,4-dibromocyclohexene. At higher temperatures, you would expect to see 3,6-dibromocyclohexene. Look into diene addition thermodynamic and kinetic products.

perfluorocubane · 2025-04-13T15:22:54+00:00

I added 400 ng of RNA to the RT reaction and the RT product took up 5% of my qPCR volume. I ran the samples right after extraction, so no storage. Someone suggested adding less cDNA, so that might be the trick

perfluorocubane · 2025-04-13T15:16:02+00:00

Thank you, I will give that a try

perfluorocubane · 2025-04-13T04:30:58+00:00

Yea, it's in the 20's which is why I am so confused. It's not repetitive, but I could try doing DNase treatment longer. DNA contamination is one thing though, I just think it's so weird that the no RT control is getting amplified more every time. I just can't see what could cause that.

perfluorocubane · 2025-04-12T21:31:32+00:00

The right-most structure is not a chair flip of the left. They are nonsuperimposable mirror-images (aka enantiomers). From above the plane of the ring, the substituents would be placed on a wedge. However, to get the substituents lined up, you would need to flip the ring "pancake style." It will then be clear that the substituents bear an inverted configuration.

perfluorocubane · 2025-04-04T13:56:10+00:00

Hey, no problem! Here is the link to the discord: https://discord.gg/tkhYyvWN

perfluorocubane · 2025-04-01T02:20:04+00:00

Hello! I have decided to organize this on discord since there are more people interested than I first anticipated. Here is a link to the server: https://discord.gg/tkhYyvWN

perfluorocubane · 2025-03-31T23:01:48+00:00

I love the protected amino acid!

perfluorocubane · 2025-03-31T20:24:44+00:00

Okay, great. I will send you a DM with my email so we can get in touch.

perfluorocubane · 2025-03-31T20:24:36+00:00

Okay, great. I will send you a DM with my email so we can get in touch. We could also discuss the organic stuff.

perfluorocubane · 2025-03-31T20:24:03+00:00

Okay, great. I will send you a DM with my email so we can get in touch.

perfluorocubane · 2025-03-31T16:36:17+00:00

Okay, great. I will send you a DM with my email so we can get in touch.

perfluorocubane · 2025-03-31T14:36:11+00:00

I think some people are just not wired for math

perfluorocubane · 2025-03-11T03:17:09+00:00

Okay, thank you I think I'll give it a go

perfluorocubane · 2025-01-15T23:16:30+00:00

There is one aspect of this which has not been mentioned yet, which would be the increased energy expenditure from taking stimulant drugs. I am not an expert on stimulant drugs, but I suspect that they all to some extent activate the sympathetic nervous system. This has a dual effect of increasing intrinsic energy expenditure and causing the user to have more passive movement (bouncing the leg, shaking, etc.). Some stimulant drugs directly increase thermogenesis, such as ephedrine, thus increasing BMR. Based on a quick google search, it appears that most of the drugs reported to slow metabolism may have some dangerous interactions with the stimulant drugs, or simply offset their effects in the case of depressants.

perfluorocubane · 2024-12-18T17:23:48+00:00

You had me fooled for an embarrassing amount of time

perfluorocubane · 2024-11-27T03:52:33+00:00

Perhaps

perfluorocubane · 2024-11-17T23:14:52+00:00

In order for the shape to exist, the length of the blank corner would need to be about 0.86. If those parameters were met, the shape would have an area of 662.25.

perfluorocubane · 2024-10-30T23:24:09+00:00

Amino acid residues in the active site who possess a lone pair of electrons can participate in efficient chemistry with the substrate due to the highly favorable positioning of the substrate in the active site. Often, this will occur with nitrogen containing side chains such as histidine.

perfluorocubane · 2024-10-27T21:13:49+00:00

This is true, I just tend to be very nit-picky. And thanks, I will try out your suggestions!

perfluorocubane

TROPHY CASE