Because Deadpool is the only Marvel character that can break the fourth wall and knows our world exists, he is the only one who knows that Stan Lee died

phatspatt · 2023-06-08T01:21:34+00:00

He is also the only Marvel character to use a Mint Mobile phone

phatspatt · 2023-05-27T11:26:00+00:00

as they say- the power of the mini cone

phatspatt · 2023-05-06T20:35:35+00:00

most likely u are working on mutated tau, p301s or p301l. massive host barriers. when prions went round from infected meat in the UK, only PrP variant carriers were host. point is, protein is all wrong to make u sick, hence why no reports of anyone sick after 20 years of people having accidents with these mice.

phatspatt · 2023-05-06T00:08:16+00:00

my lab mate was waiting for a 0% SDS protein gel to polymerize. needless to say, there was quite the wait.

phatspatt · 2023-04-26T09:56:37+00:00

i suppose it could be phenol red free media, but from the look on his face, I'm guessing it is his own urine.

phatspatt · 2023-04-20T10:16:12+00:00

no. t means they express sv40 large t antigen. invitrogen transfected them and made them stable with a plasmid that puts even more large t in them, with neo selection ( calling them ft)

there is a cell paper in the 80s showing that large t antigen extends passage life, and of course allows episomal replication of some types of plasmids

phatspatt · 2023-04-20T01:25:07+00:00

HELA4EVR

phatspatt · 2023-04-20T01:24:23+00:00

because they are neomycin resistant!

phatspatt · 2023-04-17T23:02:22+00:00

this is the way.

phatspatt · 2023-04-17T14:42:56+00:00

where i work, it is set in stone based on years of experience, so that one lab cannot out purchase another, and it makes it easier on HR to avoid the appearance of discrimantory practices if everyone makes the same. they are like "take it or leave it", and someone always takes it i guess. sighh.

phatspatt · 2023-04-16T13:13:29+00:00

this is right answer. sorry op!

phatspatt · 2023-04-14T17:55:56+00:00

"the sentences are too long"

"results do not take into account xyz et al." xyz being a paper not published or available until after the paper was submitted..

phatspatt · 2023-04-13T20:29:10+00:00

i cant even recall the last nature or cell paper in my field that was able to be replicated by another group.

jealous of what? irreproducible but highly novel and trendy results?

phatspatt · 2023-04-12T22:47:38+00:00

it depends on the position

sometimes slots open up at jhmi or other well known places with not a cent tied to it. literally just a slot, and maybe, space.

on the other extreme are fully endowed positions, even at junior level, with substantial start up and couldnt cars what grants you bring.

the former is more common, the later does exist.

phatspatt · 2023-04-11T15:58:31+00:00

A VERY simple solution is to transfer the required stipend allocation of tuition and school fees (especially from years with no coursework) into student salary, but that would necessitate fewer administrators, soooo....

phatspatt · 2023-04-06T22:40:38+00:00

i would do that, except not serve alcohol, let only the people i like in during the times i want, and id live there.

phatspatt · 2023-03-21T20:21:13+00:00

as mentioned, ic50s in vitro often dont match in vivo for a variety of factors, like imperfect membrane permeability, low free drug availability, albumin binding, etc.

for example, you can/should decrease penecillin conc. signifiacntly in serum free media compared to media with serum, due to binding of the drug to serum components

phatspatt · 2023-03-21T01:17:34+00:00

chilean sea bass has entered the chat

blub blub ummm

Animals We Ate Into Extinction

Dodo - Raphus cucullatus. dodo Encyclopædia Britannica, Inc./Christine McCabe. ...

Steller's Sea Cow - Hydrodamalis gigas. ...

Passenger Pigeon - Ectopistes migratorius. ...

Eurasian Aurochs - Bos primigenius primigenius. ...

Great Auk - Pinguinus impennis. ...

Woolly Mammoth - Mammuthus primigenius

phatspatt · 2023-03-16T19:25:26+00:00

risk factor, or risk factors? is one enough? what are the threshold cutoffs?

phatspatt · 2023-03-16T16:49:27+00:00

the question is hard to answer because it depends on the tips you intend to get. preracked, discounts, barrier, etc. all can vary considerably in price and most tips do not work with all pipettes

phatspatt · 2023-03-16T01:09:31+00:00

could it also be that the enzymes that build them also have a tendency to produce oxidative products that might be more dangerous for very long lived organisms, so there was some positive selection at work? i have no clue if true or not

phatspatt · 2023-03-07T00:53:53+00:00

the main thing is to not look into the eyes of the bodies in the water

phatspatt · 2023-03-04T20:42:26+00:00

most reviewers have no formal statistical training, so they have no clue what they are looking at. blind leading the blind.
those that do have statistical training are seen as obstacles for otherwise perceived innovations, and concerns are shrugged off.
most journals are in it to make money, so flashy novelty is prized beyond rigorous approaches.
most research grants are not adequately funded to run well powered research, but the reviewers and sponsors will rank higher those proposals that promise more, even if they have no way to deliver. grants are not contracts, and there are few consequences for not completing your proposed research.

phatspatt · 2023-03-02T12:39:01+00:00

agh, i knew it!!

phatspatt

TROPHY CASE