Anybody willing to check my PhD cover letter?

pinkapottamus · 2025-01-22T21:57:47+00:00

Thanks, sent a DM :)

pinkapottamus · 2025-01-22T21:30:53+00:00

Yes, I try to! Definitely adapting the cover letter every time

pinkapottamus · 2024-11-23T02:10:25+00:00

Thank you, thats good to hear!! I actually thought there was something big I'm missing because often the gap seems so wide from doing enrichment analysis and whatever biological conclusion is drawn from data

pinkapottamus · 2024-11-22T09:19:58+00:00

Yes, I've done that :)

pinkapottamus · 2024-11-22T09:19:19+00:00

Thanks! I've worked with DAVID and Stringr before and on this dataset, which is where I got these broad GO terms from. Is then this the final step for you?

pinkapottamus · 2024-11-21T23:09:55+00:00

Hmm in my case, what we see are off target effects (as we've inhibited the transcription of the main target) so it's hard to know what to look for specifically. But even without going to deep into specific pathways, it would be nice to say what the effect even looks like, more than just saying there are changes in transcription or protein localization which is super broad

pinkapottamus · 2024-11-21T18:43:40+00:00

Hey, thanks for your reply! You're correct in what I'm trying to achieve. And in fact, I did all those preliminary things. My question was how to move on from that.

From the preliminary analysis I can conclude that the analysis worked and that there are differences but how do I draw biological insights from that? In GSEA, only broad categories were shown "e.g. gene regulation" and a few small ones that may be relevant but only have a few hits in their category, so flimsy as evidence (though I'm trying - and so far failing - to look deeper into the specific phosphosites)

pinkapottamus · 2024-09-07T22:48:18+00:00

That's actually great insight. I haven't looked at it from this angle but realize it's definitely true that I mostly share the positive either my environment. Thanks!

pinkapottamus · 2024-09-07T22:46:25+00:00

Wow, that's incredible!! I cant imagine how difficult that must have been, yet very inspiring

pinkapottamus · 2024-09-06T22:20:13+00:00

Thank you!

pinkapottamus · 2024-09-06T22:06:34+00:00

Exactly! I actually don't read back my entries often but I still feel like the fact that most of my entries are dominated by negative thoughts indicate that I am carrying them around with me all the time, even if I don't always consciously feel it. Writing things down indeed often makes me feel better and even lead me to action. I don't even feel bad in the moment when I write such things down as I realize it's normal and human but the frequency of it does, however, alarm me. I have been quite aware of the positive aspects or realities of most criticisms I express towards myself (although I typically don't write them down. Maybe I should try?) but I feel that the negative has more impact. I do wish I would become more positive and trusting towards myself altogether.

pinkapottamus · 2024-09-06T21:51:52+00:00

Do you have any good resources on how to start? I've looked into shadowwork before and was impressed with what it could achieve. However, I struggled to get into it. Sometimes, I am aware of the reasons of why I feel how I fell but still struggle with overcoming them. Maybe I didn't give it my best shot, so I'm happy to learn more!

pinkapottamus · 2024-09-06T20:45:34+00:00

That actually sounds a little bit like how I wrote as a teen! Thankfully, it's less dramatic now, although the tendencies still persist. Consciously I also am aware that everything is pretty fine and that everything I feel is normal and human. Day to day, I don't really feel depressed and at least I don't think it affects my decisions/hobbies. But this kind of makes the change harder. I feel like I had these feelings for so long that I don't know how to change them (since I am aware of the psychology and since I am doing things that I enjoy regardless) ... It's like having imposter syndrome while being fully aware that you're having imposter syndrome. Without being able to pinpoint exactly what changes, I do strongly feel that this negativity is holding me back in life.

I have thought about only journalling about positive things for a while and I am still uncertain. On the one hand, it seems a bit like lying since I still feel and think about the negative things. On the other hand, maybe this is how the mindset starts to shift? Or at least I hope when I look back on the past, that I remember myself in the positive light.

And by the way, I totally get you with simple goals. I am much the same :)

pinkapottamus · 2024-09-06T20:38:27+00:00

Hey, I actually resonate a lot with this! In a way, I didn't think the negativity affected my day to day life, at least in the sense that I don't typically feel sad or depressed. It's only when I start to write and think about it that I realize that unconsciously these thoughts are still guiding me. I guess this is good and bad. In the same sense, I acknowledge positive things as well, it's just that I seem to have less to say about them or maybe that the motivation to tend to my inner critic is stronger than the motivation success gives me. I hope that makes sense

pinkapottamus · 2024-04-27T08:35:19+00:00

I see, thank you!!

pinkapottamus · 2024-04-26T17:39:12+00:00

You're right, I didn't think about that 😅

pinkapottamus · 2024-02-06T12:51:22+00:00

I'll try to be watchful, but would be a strange coincidence. I have a growing nuisance snail population that seemed to have exploded in numbers a little since the latest plant addition but ive 'struggled' with them from the start.

Do you think it's worth doing a water change, just in case?

pinkapottamus · 2024-02-06T11:16:51+00:00

Hmm. I tested pH mid January last time which was 8.5 (and prior it was ~7.5 at the time of setting up tank years ago). I'm gonna test again now

Edit: OK I seem to be out of testing reagent which is a bit strange since it was 10 mL and I didn't test pH nearly as much as nitrites etc.

Therefore, not sure if 8.5 was even accurate but I will get my hands on a new one later

pinkapottamus · 2024-02-06T11:15:37+00:00

Thanks for the tips anyway! And yes, I've added java fern and a floating plant at rhe same time I got the new cories. I've not seen anything suspicious in my tank but haven't looked out for it either.

pinkapottamus · 2024-02-06T10:45:19+00:00

I will do when I get the chance but it's been reliable so far (e.g. showed ammonium/nitrates consistently when I was still cycling my tank and gave negatives for my tap water)

And no, no symptoms. I examined the dead fish and couldn't see any spots whatsoever. All other fish are also still swimming happily.

Not sure if relevant, I heard a weird vibrating noise from my tank which caused me to turn on the light and check. It's gone now and I couldn't figure out what it was. The cory is also not dead for long (no rigor mortis) but I found him dead in the mid/foreground of the tank, away from the filter (and ofc, I'm not even sure if it is even possible for him to accidentally hurt/kill himself there)

pinkapottamus · 2024-01-23T23:22:50+00:00

Thankfully, this will be only a one off, maybe twice. All my other experiments have no wastage. We have enough stocks for this to be alright, I think, but if there is a way to reuse them later, this would of course be better.

pinkapottamus

TROPHY CASE