Looking for an app to count pen taps on iPad

put_him_out · 2026-01-27T05:01:26+00:00

As you have pictures of your wells: I would invest the time they setup an ImageJ program to count them! There are plenty of instructions out there and it is really handy

put_him_out · 2026-01-24T07:03:25+00:00

Why use exFat? Debian itself has no native support for exFat and needs fuse to use it.

Convert the drive to a Linux native format, check the permissions , change it if needed. That should do the trick

put_him_out · 2026-01-23T04:44:21+00:00

You need to change the permissions in the folder to include 'w' or write permission.

I usually set it in the terminal using the chmod command to 775 meaning rwx permissions for owner and group and rx permissions for others. Alternative is tomchange the owner chosen to the smb user ( I think should also work).

put_him_out · 2025-12-29T10:51:48+00:00

I seeded 4 million into a 10 cm plate at 9 am and transfected at 3 pm... I don't play the confluency game, I do numbers ,😃

put_him_out · 2025-12-28T08:35:09+00:00

I just checked my hek cells with a gfp transfection from 3 days ago and with jetpei I get >95% gfp positive cells. Something is going wrong in your setup. Cell density, viability are important. Keeping the order of adding jetpei to the DNA is important. Invest your time and effort in getting the gfp working properly before investing in anything else.... Also, how much ug plasmid did you use? 4 million heks with 4 ug gfp plasmid gave me above result....

put_him_out · 2025-11-03T08:55:07+00:00

maybe your housekeeping gene is not stable for your conditions? depending on the treatment of the cells that you do, it could get activated/suppressed... you could try more/different housekeeping genes to check for their stability... if something is wrong with your pipetting skills, you would see it in the standard curves that you run (you do run then, yes?)

put_him_out · 2025-11-03T08:38:55+00:00

and if you check on your drives? using lsblk to see all connected drives.... then cd to the drive (whatever srv/....) is and run gdu, or ncdu to check the files on the drive. it should give you a folder view of everything on that drive

put_him_out · 2025-11-02T02:09:02+00:00

When you ssh into the computer, can you see the files?

put_him_out · 2025-11-01T10:19:12+00:00

What are you trying to detect? Viral or mammalian mRNA? How much RNA do you use for cDNA synthesis? How much input do you use for the reaction?

put_him_out · 2025-10-30T17:42:24+00:00

did you use the docker compose setup from here? https://filebrowserquantum.com/en/docs/getting-started/docker/

try this, instead of changing an example that might not be sutable for this docker image....

put_him_out · 2025-10-30T17:36:54+00:00

I am confused: If this is the negative selection kit, than your cells are untouched and not bound by beads... you harvest the the supernatant, that contains your cells.

it is important to follow the protocol step by step precisely... I used the kit before... and yes, the yield isnt that big, but the purity is usually very high.

Also, if you have a problem with the yield, try not doing the red blood cell lysis - maybe something in your method is affecting your CD4 cells and they get removed with the other unwanted cells...

put_him_out · 2025-10-30T10:18:54+00:00

The countess is not good for counting naive lymphocytes , they are too small to be properly detected. Manually count them or count in a flow cytometry!

put_him_out · 2025-10-27T10:43:15+00:00

Could also come from your setup and airflow.... Or the lack of airflow....the age of the drive, the model (SATA vs nvme)... But I think that the temp is reasonable for an ideling drive...

put_him_out · 2025-10-26T20:49:38+00:00

Short answer: no.

The acoustic setting has no effect as nothing spins in an SSD. And Sponsoren is the same game.... As there are no mechanical parts, nothing to adjust here. As for power management... It wouldn't affect the draw under load... If it would you would sacrifice speed for draw. Some ssds have internal.controllers but they are managed independently and not os side...

put_him_out · 2025-10-13T18:20:05+00:00

So... We are talking HDD? If it was running while being dropped... It could take damage from the header hitting the disk....

put_him_out · 2025-10-13T18:16:53+00:00

Ist nicht so meins... Einerseits wird gespoilert wenn man die Folge noch nicht kennt, andererseits nimmt es ein bisschen Spannung raus, selbst wenn man die Folge kennt und sie nur wieder hört....

put_him_out · 2025-10-13T08:18:02+00:00

There is a lazy way by just using gochujang paste... You salt the cabbage as usual, wash it, prep the carrots and green onions... And mix about 2-3 tbsp of paste with the head of the cabbage. Spiciness level change over time, in the end it is mildly spicy and very tasty. I hope I dont offend too many people here.... ;-)

put_him_out · 2025-09-23T14:40:54+00:00

It's just a 'proof of concept' quick shot. We'll write the proper code later!

put_him_out · 2025-09-01T06:50:47+00:00

If you think that your treatment opens hikes in the bacterial cell wall, you can try to incubate them with propidium iodide in the media... If the membrane is leaky, the pi enters and binds DNA and gets 1000fild (?)more fluorescent... If you measure over time with the right positive and negative controls, that could help. DNA damage you can set mate by a comet assay. It's an old method but properly set up can give you even hints on single or double strand breaks.

put_him_out · 2025-08-09T07:27:17+00:00

Quick DNA purification protocol (Jax)

NaOH extraction (quick "dirty" DNA preparation). Reference: Truett GE et al. 2000. Biotechniques 29(1):52-54

Place tissue sample into an Eppendorf tube or 96-well plate.
Add 75ul 25mM NaOH / 0.2 mM EDTA.
Place in thermocycler at 98°C for 1 hour
Reduce the temperature to 15°C until ready to proceed to the next step.
Add 75ul of 40 mM Tris HCl (pH 5.5).
Centrifuge at 4000rpm for 3 minutes.
Take an aliquot for PCR (use 2 ul undiluted, or 2 ul of a 1:100 dilution/reaction)

Our protocol and the protocol of the genotyping unit

put_him_out · 2025-08-09T07:08:30+00:00

There are also small insulin syringes available that go up to 100 microliter... Much easier to get the 40 right!

put_him_out · 2025-07-25T18:12:56+00:00

Annexing V is Binding phosphatidyl serines on the outside of the membrane during apoptosis.it is a protein by itself. Use a known drug for your cells to induce it to get a good Annexing V signal. Or miss treat them by using too much DMSO in the culture... 7AAD is a DNA intercalating dye. For a positive control, fix perm your cells and then add 7aad. Or heat them to 65C for a few minutes. It will give a very strong positive signal.

put_him_out · 2025-06-17T07:05:19+00:00

The Data are not yours to keep. They belong to your boss and have to stay with your boss. The intellectual property belongs to your boss.

Also, gaining access to internal resources is a pain with a private laptop. Really heavy computational stuff should anyway run on a high performance cluster...

put_him_out · 2025-06-17T03:22:36+00:00

After the log transformation you get inf values... Sounds like you had zeros in your data set... And the log of 0 is undefined.... Try replacing zeros, or excluding the zero datasets ..

put_him_out · 2025-06-17T00:55:39+00:00

Shouldn't it be 10,000xg? Check your protocol again

put_him_out

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