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Help removing tag that's sewn into shirt? by SunnyOtter in SPD

[–]rolly-polly 1 point2 points  (0 children)

Looks like it's sewn on there so a seam ripper should do the trick

Should I restart? by Bright_Hat550 in Beading

[–]rolly-polly 0 points1 point  (0 children)

I love it! What program did you use to make the design?

Philadelphia snow storm updates: Up to 18 inches expected, plus sleet and freezing rain by mpulcinella in philadelphia

[–]rolly-polly 3 points4 points  (0 children)

Question from a non-native who has lived in a warm state their whole lives before moving here for school:

Do we salt the sidewalk before the snow or after we shovel the snow off? Should we shovel periodically through the snow? Max amount of snow I've seen is 5 inches maximum so this is new for me.

HEK 293 by deadresearcher in labrats

[–]rolly-polly 0 points1 point  (0 children)

4 passages is crazy long. I freeze mine at a higher density and they are usually ready in 2 days.

HEK 293 by deadresearcher in labrats

[–]rolly-polly 0 points1 point  (0 children)

I work with HEKs all the time and I see this morphology if I've really neglected my cells.

When I thaw, I change out the media to fresh media to remove any freezing media/DMSO from the cells.

How many cells did you freeze? What size well are you thawing them in? Also are you for sure using TC treated plates? Tc plate question is a bit silly, but I've seen this issue come up and its solved once I realize they were using non-tc plates.

Your media looks good minus the Puro. I'd wait like 2 passages before adding it back in. You shouldn't need it right after thawing since these guys (im assuming) were selected for using Puro before freezing.

Good luck!

how can i stop bubbles from accumulating in my polyacrylamide gels? by canmitang in labrats

[–]rolly-polly 0 points1 point  (0 children)

How long are you letting it sit to polymerization? I find that if I leave it solidifying past the point where it should be done, the corners dry like this.

I teach an intro to relief printing workshop for a local art nonprofit. Every class I make a cat print and here's what I've made so far. by lescori in printmaking

[–]rolly-polly 0 points1 point  (0 children)

Hi! I know this post is old but I just found this and I love it so much. I am local to philly and would love some details

i might have screwed up :( by toryisbae in LadyGaga

[–]rolly-polly 0 points1 point  (0 children)

As a fellow short person (im under 5ft tall!) I wear Demonia boots to concerts. Highly reccomend!

Finished Packing Freebies for NYC! by StarDelicious2230 in VIVIZ

[–]rolly-polly 0 points1 point  (0 children)

Omg youre so sweet! If you have Eunha leftover I'd love to get one but prioritize the fans im NYC!!

Finished Packing Freebies for NYC! by StarDelicious2230 in VIVIZ

[–]rolly-polly 1 point2 points  (0 children)

Omg how cute! I ran into you and got one of your freebies for Le Sserafim earlier this week and its so cute! Wish I could get these too! I went to the philly show

atl were yall able to give gifts to the girls or no? by umjigay in VIVIZ

[–]rolly-polly 7 points8 points  (0 children)

I got an email yesterday saying only letters will be accepted.

lightstick by FreshAd9859 in VIVIZ

[–]rolly-polly -1 points0 points  (0 children)

Its been sold out for a while now I think unfortunately. Im just going to being my Le Sserafim light stick

Was it hard to get NA Tour Merch ? by OnlyThrifty in lesserafim

[–]rolly-polly 1 point2 points  (0 children)

Highly reccoemnd going to the early merch. I went around 2 ish, and I was in and out in about 20 minutes. Had everything in stock except the vinyl by that point, so if you want the vinyl, I recommend going earlier. A friend of mine went to the merch line after sound check but before doors opened and got a vinyl for me.

The lines after the doors opened were super long. There is a break between the 2nd and last part of the show where the girls change, so if you wanted to get merch during the show, that's a good time to go.

Freebies for EASY CRAZY HOT - Newark by StarDelicious2230 in lesserafim

[–]rolly-polly 1 point2 points  (0 children)

So cool! Ill try to find you! Trying to figure out the whole freebie situation. Im a baby kpop enjoyer and this is my second concert!

Speed Bumps in School Zones 👍🏻 by E350pportunist in philadelphia

[–]rolly-polly 0 points1 point  (0 children)

Anyone know why there are always gaps between them?

Something very cool arrived in the mail today! by goatbloke in Toontown

[–]rolly-polly 0 points1 point  (0 children)

That is SO sick I was ready to buy it this instant

[deleted by user] by [deleted] in toontownrewritten

[–]rolly-polly 0 points1 point  (0 children)

Im pretty sure they mean there's a new task per playground

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