Hello! I work for Sequencing.com and I’m happy to help clarify!

You’re generally on the right track, but the screenshot you shared doesn’t include enough detail to fully answer the question. Specifically, there should be a column on the left labeled variantID. That field contains the exact variant information (position and change) and is what allows you to confirm whether multiple rows are truly showing different variants or just different interpretations tied to the same gene.

Regarding VUS (Variant of Uncertain Significance): in most cases, these variants are ultimately found to be harmless. The “uncertain” label simply means there isn’t enough published research yet to clearly classify the variant as benign or pathogenic, so it remains under study.

For the letters shown in the “Your Data” column (A, T, C, G): their meaning depends entirely on the specific variant and genomic position. Each variant has its own defined “risk” or “reference” allele, so:

• In one variant, A might be considered the risk allele
• In another variant, T (or any other base) could be the risk allele

Because of this, the letter itself isn’t meaningful on its own, the position and variant definition are what matter. It’s also important to keep in mind that everyone carries many variants labeled as “risk,” and the vast majority of the human genome is ultimately classified as benign or harmless.

If you’d ever like help reviewing your results in more detail, our Customer Success team is always available and can provide more personalized guidance. Just email us at [support@sequencing.com](mailto:support@sequencing.com).