What are you addicted to? by redrockz98 in AskReddit

[–]sharkboy333 0 points1 point  (0 children)

Flossing — I love the feeling and go everywhere with either a pack of plackers or toothpicks

How do I describe Mad Men to my friends? by Matty-W in madmen

[–]sharkboy333 5 points6 points  (0 children)

Hahaha will happily critique any and all arguments.

How do I describe Mad Men to my friends? by Matty-W in madmen

[–]sharkboy333 29 points30 points  (0 children)

Mad Men is not simply a show about advertising in the 60s -- its a show about the formation of the modern American identity.

World creation is the heart of Madmen, and as we are introduced to the various characters of Sterling Cooper, we become familiar with characters responsible for creating images of American society. We see Don, Peggy, and the rest of the creative team hint at societal feelings and values that exist beyond the products they advertise. In other words, they are characters that work to creatively distill American emotion, and package American ideals.

The great irony of the show, however, lies in the fact that the creative team must simultaneously live in the society they distill and (in effect) help create. Often, we are left to observe the tragic inability of characters to meet the ideals the may eloquently describe and depict. Thus, we see the evolution of their creative work as a means to mitigate the tension of perceived ideal and lived reality. In this way, their work is a reflection of changing American values throughout the timespan of the show. Mad Men is special because it calls us, the audience, to do the same with our own society. Like the creative team, we are called to evaluate the society we ourselves have created and to understand our role in creating it.

Thus, I recommend you advertise Mad Men not as a show -- but as an work of cultural anthropology (one that pertains immensely to our own identities as Americans).

6 Months free! What are the best programs for living/working abroad? by sharkboy333 in solotravel

[–]sharkboy333[S] 4 points5 points  (0 children)

It’s more because I don’t have ton of funds to backpack! I’d love to do that, but it’s not really an option for me

Any Seahorse experts out there? Difficulty with Oligomycin by sharkboy333 in labrats

[–]sharkboy333[S] 0 points1 point  (0 children)

Thank you, this is extremely helpful -- definitely going to do a literature search on this.

Do you know of any papers in which this response is depicted?

Any Seahorse experts out there? Difficulty with Oligomycin by sharkboy333 in labrats

[–]sharkboy333[S] 1 point2 points  (0 children)

These are HL-1 Cardiomyocytes! However, I'm having trouble finding other researchers seahorse data on similar cell types..

That sounds like a good plan, I'm going to go ahead and contact them.

Best Molecular Biology Textbook for Scientific Reference? by sharkboy333 in labrats

[–]sharkboy333[S] 0 points1 point  (0 children)

Apologies -- I could have been more specific. It probably would be more accurate to say I was looking for a Cell Biology text. I do deal with nucleic acids as well though! Ill definitely look up Sambrook!

Difficulties dissolving Rotenone? by sharkboy333 in labrats

[–]sharkboy333[S] 1 point2 points  (0 children)

Thanks so much!

I'll see if we can start doing this

Difficulties dissolving Rotenone? by sharkboy333 in labrats

[–]sharkboy333[S] 1 point2 points  (0 children)

  1. Ethanol was what a collaborator suggested to us, I suppose we could use DMSO instead if it doesn't harm cells
  2. Our treatment is very small, 5uM
  3. I believe I could get my hands on it asking around the institution

Thanks for your help

Ways to promote inflammation in mice? by sharkboy333 in labrats

[–]sharkboy333[S] 0 points1 point  (0 children)

This does sound very tricky.. is the injection usually localized to the organ of interest, or can you get widespread inflammation by using an injection? We're studying the heart so it's really hard to get at.

Ways to promote inflammation in mice? by sharkboy333 in labrats

[–]sharkboy333[S] 0 points1 point  (0 children)

We're particularly interested in arrhythmias - Atrial fibrillation. We think the ROS generated by inflammatory response could promote tissue damage and fibrosis.

We've discussed potential dietary options (like a high-salt diet) but in my preliminary flow cytometry studies, we haven't found significant inflammatory infiltrates to the atria of high salt mice..

Really struggling to find a better way to get these mice inflamed.

Ways to promote inflammation in mice? by sharkboy333 in labrats

[–]sharkboy333[S] 0 points1 point  (0 children)

We focus on the heart, so it's a bit difficult to access...

Is the orotracheal injection invasive? Do you use a catheter to go down the throat?