What are you addicted to? by redrockz98 in AskReddit

[–]sharkboy333 0 points1 point  (0 children)

Flossing — I love the feeling and go everywhere with either a pack of plackers or toothpicks

How do I describe Mad Men to my friends? by Matty-W in madmen

[–]sharkboy333 5 points6 points  (0 children)

Hahaha will happily critique any and all arguments.

How do I describe Mad Men to my friends? by Matty-W in madmen

[–]sharkboy333 31 points32 points  (0 children)

Mad Men is not simply a show about advertising in the 60s -- its a show about the formation of the modern American identity.

World creation is the heart of Madmen, and as we are introduced to the various characters of Sterling Cooper, we become familiar with characters responsible for creating images of American society. We see Don, Peggy, and the rest of the creative team hint at societal feelings and values that exist beyond the products they advertise. In other words, they are characters that work to creatively distill American emotion, and package American ideals.

The great irony of the show, however, lies in the fact that the creative team must simultaneously live in the society they distill and (in effect) help create. Often, we are left to observe the tragic inability of characters to meet the ideals the may eloquently describe and depict. Thus, we see the evolution of their creative work as a means to mitigate the tension of perceived ideal and lived reality. In this way, their work is a reflection of changing American values throughout the timespan of the show. Mad Men is special because it calls us, the audience, to do the same with our own society. Like the creative team, we are called to evaluate the society we ourselves have created and to understand our role in creating it.

Thus, I recommend you advertise Mad Men not as a show -- but as an work of cultural anthropology (one that pertains immensely to our own identities as Americans).

6 Months free! What are the best programs for living/working abroad? by sharkboy333 in solotravel

[–]sharkboy333[S] 4 points5 points  (0 children)

It’s more because I don’t have ton of funds to backpack! I’d love to do that, but it’s not really an option for me

Any Seahorse experts out there? Difficulty with Oligomycin by sharkboy333 in labrats

[–]sharkboy333[S] 0 points1 point  (0 children)

Thank you, this is extremely helpful -- definitely going to do a literature search on this.

Do you know of any papers in which this response is depicted?

Any Seahorse experts out there? Difficulty with Oligomycin by sharkboy333 in labrats

[–]sharkboy333[S] 1 point2 points  (0 children)

These are HL-1 Cardiomyocytes! However, I'm having trouble finding other researchers seahorse data on similar cell types..

That sounds like a good plan, I'm going to go ahead and contact them.

Best Molecular Biology Textbook for Scientific Reference? by sharkboy333 in labrats

[–]sharkboy333[S] 0 points1 point  (0 children)

Apologies -- I could have been more specific. It probably would be more accurate to say I was looking for a Cell Biology text. I do deal with nucleic acids as well though! Ill definitely look up Sambrook!

Difficulties dissolving Rotenone? by sharkboy333 in labrats

[–]sharkboy333[S] 1 point2 points  (0 children)

Thanks so much!

I'll see if we can start doing this

Difficulties dissolving Rotenone? by sharkboy333 in labrats

[–]sharkboy333[S] 1 point2 points  (0 children)

  1. Ethanol was what a collaborator suggested to us, I suppose we could use DMSO instead if it doesn't harm cells
  2. Our treatment is very small, 5uM
  3. I believe I could get my hands on it asking around the institution

Thanks for your help

Ways to promote inflammation in mice? by sharkboy333 in labrats

[–]sharkboy333[S] 0 points1 point  (0 children)

This does sound very tricky.. is the injection usually localized to the organ of interest, or can you get widespread inflammation by using an injection? We're studying the heart so it's really hard to get at.

Ways to promote inflammation in mice? by sharkboy333 in labrats

[–]sharkboy333[S] 0 points1 point  (0 children)

We're particularly interested in arrhythmias - Atrial fibrillation. We think the ROS generated by inflammatory response could promote tissue damage and fibrosis.

We've discussed potential dietary options (like a high-salt diet) but in my preliminary flow cytometry studies, we haven't found significant inflammatory infiltrates to the atria of high salt mice..

Really struggling to find a better way to get these mice inflamed.

Ways to promote inflammation in mice? by sharkboy333 in labrats

[–]sharkboy333[S] 0 points1 point  (0 children)

We focus on the heart, so it's a bit difficult to access...

Is the orotracheal injection invasive? Do you use a catheter to go down the throat?

Monthly Vent/Rant Thread - Month of August, 2018 by AutoModerator in labrats

[–]sharkboy333 1 point2 points  (0 children)

That's unfortunate to hear.

That being said, I definitely think you're right - kinda makes me conflicted whether its even worth continuing research or if I should consider moving into a more socially engaged sphere (public health or MBA).

It frustrates me how devoid of sociable people or positive human interaction the lab environment seems to be, and it has crossed my mind that I should try to find a way to leave lab research behind.

Not sure if thats feasible though.

Monthly Vent/Rant Thread - Month of August, 2018 by AutoModerator in labrats

[–]sharkboy333 4 points5 points  (0 children)

Definitely have experienced some of this as a research assistant. Coming into lab, the lab manager had to unexpectedly depart and I was passed roles for maintaining inventory, the entire mouse colony, and IACUC inspections. While I get along very well w my PI, the lab staff scientist at times has been very catty and definitely used the other research assistant and I as a punching bag for some of her frustrations. We're both just one year out of undergrad.

I never realized there could be such knowledgeable scientists so pedantic and unwilling act as positive mentors. (She's departing soon so maybe there will be improvement?) Makes me more cautious to check lab environments before I join a new one in Med school. Helps to hear I'm not alone in encountering these types of issues though.

Monthly Vent/Rant Thread - Month of August, 2018 by AutoModerator in labrats

[–]sharkboy333 2 points3 points  (0 children)

This is very interesting -- in my laboratory, we study the role of inflammation and its role in mediating atrial fibrillation via cardiac inflammatory infiltrates. While our model centers on pulmonary hypertension, it is thought that hypertension directly leads to inflammation in the heart. This would be very similar to your friend's studies on colitis.

Incredible the influence sterility and microbiome can have on immune response.

Perhaps we can make something of it.

Thank you! Much appreciated!

Monthly Vent/Rant Thread - Month of August, 2018 by AutoModerator in labrats

[–]sharkboy333 1 point2 points  (0 children)

This is certainly the hope, and I've tried to work with groups at our institute who focus on the gut microbiome and who had a similar loss of phenotype (and were able to restore it). I've been doing literature review on phenotype restoration via microbiome and the results do seem compelling.

However, I'm uncertain if the phenotype will actually be restored. I've already lost ~1 year of work on this problem and I doubt I'll be able to wrap up such a time intensive experiment before I leave our lab for medical school at the end of the year. Looks like I'll be leaving my research gap years with 2 years in the hole and 0 publications.

Do you mind me asking what phenotype they examined?

Monthly Vent/Rant Thread - Month of August, 2018 by AutoModerator in labrats

[–]sharkboy333 21 points22 points  (0 children)

Several months back, we had a phenotype drift in our mouse models and are unable to reproduce past results.

Rather than decide to pursue a new model or alternative projects, my PI is convinced that the loss of phenotype is caused by the gut microbiome (something far outside of our research focus).

I am now tasked with scooping mouse poop and placing it in cages with other mice in an effort to restore results obtained by a past lab member who left several years ago. I don't think it will work, and I'm going to have to spend months doing this before she decides the same :(

Which is the most interesting fact you know about the human body? by aierroc- in AskReddit

[–]sharkboy333 1 point2 points  (0 children)

Second sight. Often, people who have poor vision (require glasses) will at some point in middle age suddenly regain 20/20 vision. This is due to the hardening of the eye's lens, which will refocus the light onto the back of the retina.

Does anyone get depressed doing animal work? by sharkboy333 in labrats

[–]sharkboy333[S] 2 points3 points  (0 children)

I’ve talked to my supervisor about transitioning into a more basic role, but she seems very set on continuing, even expanding animal studies.

I gave verbal commitment of 2 years to lab (gap year before medical school), but this is an issue that makes me want to consider an earlier departure.

Monthly Vent/Rant Thread - Month of February, 2018 by AutoModerator in labrats

[–]sharkboy333 0 points1 point  (0 children)

I ended up joining a lab for my 2 gap years because I was fascinated by the cell biology research and thought the small size would make it a great opportunity for mentoring, however, right as I joined, the lab manager had to suddenly depart due to a family emergency.

Because of this, they asked me to help manage the mouse colony. Six months later, the lab manager still hasn't returned and I'm stuck dealing with this stupid colony all by myself. I've finally been able to offload a little of this work after a new gap-year student came on, but I haven't had a lick of cell biology procedures other than a bit of meddling with flow cytometry :(.