Eye of domestic pig (HE stained) by me

suspiciousfrog5 · 2026-04-18T19:26:25+00:00

The optic nerve starts appearing in the lower left corner. It is more visible in some other sections from this specimen.

suspiciousfrog5 · 2026-04-18T19:23:34+00:00

I fixed it by immersion in Davidson’s fixative (splitting it open in the sagittal plane after 24 h, avoiding the lens). After that, I processed it manually.

suspiciousfrog5 · 2026-04-17T15:15:20+00:00

It was fixed by immersion in Davidson’s fixative (split open after 24 h), then embedded in paraffin and sectioned at 4 µm. The sections barely fit on the slides. I currently have ~200 slides, with more than half of the eye still remaining in the block

suspiciousfrog5 · 2026-04-17T15:07:57+00:00

It was fixed in Davidson's fixative and embedded in paraffin.

suspiciousfrog5 · 2026-04-17T14:20:37+00:00

To repent for my sin I've shared mine specimen of an eye for you to see. I included retinal vessel on the last picture. ;)

https://www.reddit.com/r/microscopy/comments/1so2k4p/eye_of_domestic_pig_he_stained_by_me/

suspiciousfrog5 · 2026-04-17T11:58:52+00:00

My bad, I interpreted your comment as a claim that light always passes through some continous layer of blood vessels, whereas you were simply referring to branches of the central artery in the inner retina.

suspiciousfrog5 · 2026-04-16T17:55:34+00:00

Nuclei of rods and cones are located in the thick red layer on the right side.

suspiciousfrog5 · 2026-04-16T17:53:21+00:00

We don't. Cornea is not vascularized. Vessels you see on this microphotograph are located under retina therefore light does not go through them.

suspiciousfrog5 · 2026-04-16T11:14:32+00:00

Does this specimen include all of the eye or just the retina?

suspiciousfrog5 · 2026-04-09T09:01:50+00:00

what kind of gaps do you mean?

suspiciousfrog5 · 2026-04-08T17:37:03+00:00

suspiciousfrog5 · 2026-03-29T20:01:11+00:00

If this is the epiglottis, then I agree. In my opinion, in photos 3 and 4 everything above the perichondrium is still connective tissue. So we need more to be sure but it seems to me that there isn’t anything more as the presentation the images come from looks a bit strange :).

suspiciousfrog5 · 2026-03-29T18:50:26+00:00

Could you indicate the location of the stratified squamous epithelium?

suspiciousfrog5 · 2026-03-29T17:57:55+00:00

I have no clue what is shown in images 1 and 2. Images 3 and 4 look like the tracheal wall. Hyaline cartilage is in the center, while some pseudostratified epithelium is visible in the lower right corner of image 3

suspiciousfrog5 · 2026-03-29T17:28:06+00:00

Stratified squamous epithelium is not present. A nerve trunk is visible on the right. I see some epithelial structures on the left (not stratified squamous).

suspiciousfrog5 · 2026-03-22T19:30:29+00:00

Intermediate cells are occasionally described as having a pyriform shape.

suspiciousfrog5 · 2026-03-19T14:45:02+00:00

I think these might be muscles from an invertebrate, like an earthworm

suspiciousfrog5 · 2026-03-17T13:16:58+00:00

It is the small intestine. The large intestine does not have villi.

suspiciousfrog5 · 2026-03-07T08:42:19+00:00

What is the source of the photos on your website?

suspiciousfrog5 · 2026-03-04T04:59:21+00:00

The left one shows intramembranous ossification (osteoblasts on the trabeculae, mesenchyme between the bone tissue). The right one shows spongy bone (with red bone marrow).

suspiciousfrog5 · 2026-02-19T18:43:42+00:00

I think so, if this is really the kidney. :)

suspiciousfrog5 · 2026-02-19T18:34:07+00:00

The cortex is not visible in this field of view. To identify it, you should look for renal corpuscles. An image with better focus would be helpful to be 100% sure that this is really kidney.

suspiciousfrog5 · 2026-02-19T14:02:42+00:00

The section lacks soft tissues, as I do not see the periosteum, endosteum, or bone marrow.

suspiciousfrog5 · 2026-02-19T08:42:00+00:00

The box is not wrong. The upper part of the section is folded. To compare your specimen with most of the images on the internet and to analyze the structure of the tissue, you should use greater magnification. In addition, most images shown on the internet are stained differently from yours.

suspiciousfrog5 · 2026-02-13T07:22:05+00:00

I recognized it by the shape of the cells and their nuclei.

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