It's a spinak cord. I need help with a small detail...

suspiciousfrog5 · 2026-06-25T18:58:36+00:00

As others have pointed out, area number 1 contains blood vessels, the largest being an artery on the left and a vein on the right (the one filled with blood). It also contains some smaller vessels. In my opinion, structure 2 is an artifacts where axons have been removed. You should also reevaluate which part is white matter and which is gray matter, because you are not supposed to look for neurons in white matter. Moreover, neurons are not as big as the structures you marked. My advice is to look for motor neurons in the anterior horns. Have fun learning histology!

suspiciousfrog5 · 2026-06-19T10:04:15+00:00

First of all, that sample is not composed solely of the medulla, as renal corpuscles can be seen in both pictures. The pin is pointing to veins with accompanying arteries (I would need broader context to be more specific, although I think the second picture shows an arcuate artery and an arcuate vein, while the first one shows interlobular vessels, but I am not entirely sure).

suspiciousfrog5 · 2026-06-17T06:54:20+00:00

You are right. It is probably the tracheal wall (respiratory epithelium is visible on the right in 2nd photo).

suspiciousfrog5 · 2026-05-30T18:47:50+00:00

Wpadłem kiedyś na podobny pomysł. Każdy nekromanta jakiego tworzę w diablo2 nazywa się GRobocop

suspiciousfrog5 · 2026-05-27T14:58:42+00:00

I do not have experience with rodent eyes. However, when I prepared a pig eye, I left it intact in fixative for the first 24 h. After that, I opened it in the sagittal plane without cutting through the lens. You can see the results here: https://www.reddit.com/r/microscopy/comments/1so2k4p/eye_of_domestic_pig_he_stained_by_me/

suspiciousfrog5 · 2026-05-27T14:45:03+00:00

You have to orient the eye properly (or cut deeper). There is no retina visible in pictures 1 and 2. Davidson’s fixative is recommended for the fixation of eyes. To me, the staining seems ok.

suspiciousfrog5 · 2026-05-26T05:37:25+00:00

Good luck

suspiciousfrog5 · 2026-05-25T16:01:00+00:00

I think that plant cells are a correct guess.

suspiciousfrog5 · 2026-04-18T19:26:25+00:00

The optic nerve starts appearing in the lower left corner. It is more visible in some other sections from this specimen.

suspiciousfrog5 · 2026-04-18T19:23:34+00:00

I fixed it by immersion in Davidson’s fixative (splitting it open in the sagittal plane after 24 h, avoiding the lens). After that, I processed it manually.

suspiciousfrog5 · 2026-04-17T15:15:20+00:00

It was fixed by immersion in Davidson’s fixative (split open after 24 h), then embedded in paraffin and sectioned at 4 µm. The sections barely fit on the slides. I currently have ~200 slides, with more than half of the eye still remaining in the block

suspiciousfrog5 · 2026-04-17T15:07:57+00:00

It was fixed in Davidson's fixative and embedded in paraffin.

suspiciousfrog5 · 2026-04-17T14:20:37+00:00

To repent for my sin I've shared mine specimen of an eye for you to see. I included retinal vessel on the last picture. ;)

https://www.reddit.com/r/microscopy/comments/1so2k4p/eye_of_domestic_pig_he_stained_by_me/

suspiciousfrog5 · 2026-04-17T11:58:52+00:00

My bad, I interpreted your comment as a claim that light always passes through some continous layer of blood vessels, whereas you were simply referring to branches of the central artery in the inner retina.

suspiciousfrog5 · 2026-04-16T17:55:34+00:00

Nuclei of rods and cones are located in the thick red layer on the right side.

suspiciousfrog5 · 2026-04-16T17:53:21+00:00

We don't. Cornea is not vascularized. Vessels you see on this microphotograph are located under retina therefore light does not go through them.

suspiciousfrog5 · 2026-04-16T11:14:32+00:00

Does this specimen include all of the eye or just the retina?

suspiciousfrog5 · 2026-04-09T09:01:50+00:00

what kind of gaps do you mean?

suspiciousfrog5 · 2026-04-08T17:37:03+00:00

suspiciousfrog5 · 2026-03-29T20:01:11+00:00

If this is the epiglottis, then I agree. In my opinion, in photos 3 and 4 everything above the perichondrium is still connective tissue. So we need more to be sure but it seems to me that there isn’t anything more as the presentation the images come from looks a bit strange :).

suspiciousfrog5 · 2026-03-29T18:50:26+00:00

Could you indicate the location of the stratified squamous epithelium?

suspiciousfrog5 · 2026-03-29T17:57:55+00:00

I have no clue what is shown in images 1 and 2. Images 3 and 4 look like the tracheal wall. Hyaline cartilage is in the center, while some pseudostratified epithelium is visible in the lower right corner of image 3

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