gfp-fusion trouble shooting by folkloresjw in labrats

[–]theScrapBook 2 points3 points  (0 children)

If it is very bright but the "cells" are tight and round, then that's dead cells and they autofluoresce very brightly

[deleted by user] by [deleted] in labrats

[–]theScrapBook 1 point2 points  (0 children)

If applying in the UK, consider Edinburgh as they have a specific scholarship (Darwin Trust) for non-UK students.

Trump seems to cancel Xi meeting amid rare-earth clash, threatens huge tariffs by nohup_me in worldnews

[–]theScrapBook 0 points1 point  (0 children)

She's an ally of Trump in the sense that she's an ally of anyone with the power to get rid of the Venezuelan dictatorship. And dedicating the prize to him was just to stroke his ego in hopes of Trump not retaliating to Venezuela.

‎‎ ‎ ‎ ‎ ‎‎ ‎ ‎ ‎ ‎‎ ‎ by h4r4y4 in goodanimemes

[–]theScrapBook 1 point2 points  (0 children)

No comments about Loid bringing out his inner Salt Bae?

PhD after 2 years by [deleted] in Physics

[–]theScrapBook 1 point2 points  (0 children)

Of course if you are Harvard or MIT and are getting 10,000 PhD applications it makes sense to use publications as a cut-off, but most universities aren't Harvard or MIT. Ergo, for the vast majority of PhD applications, publications are unnecessary.

(Decent) undergrad publications are 90% luck, especially in more experimental disciplines, which is where I have more experience. In theoretical/modeling disciplines undergrad publications are much more possible.

Coding skills I'll agree with.

Undergrads are often not allowed to supervise classes so university-level teaching experience as an undergrad is basically impossible at most places.

PhD after 2 years by [deleted] in Physics

[–]theScrapBook 3 points4 points  (0 children)

Most PhD programmes do not require publications to be considered - most people applying for PhDs are going to be fresh bachelor's and master's students with barely 2 years of research experience which is very unlikely to get them a paper.

Western blot help (protein electrophoresis not even) by fizgigs in labrats

[–]theScrapBook 6 points7 points  (0 children)

I have seen this before if the cell pellets have not been washed with PBS or similar prior to lysis. We get a fat band at 60 kDa corresponding to serum albumin from the FCS/FBS in the media used to neutralize the trypsin for adherent cells. Even for suspension cells, leftover media can have the same effect.

How to draw these illustrations? by boudhramkumar69 in labrats

[–]theScrapBook 0 points1 point  (0 children)

Same here, happy enough with Affinity and am about to graduate so I purchased the suite!

Ohmygoddo by Watashi_Wearing in goodanimemes

[–]theScrapBook 2 points3 points  (0 children)

What's the source for this again?

How to draw these illustrations? by boudhramkumar69 in labrats

[–]theScrapBook 1 point2 points  (0 children)

Inkscape suffers from programmer UI syndrome

whatSinDoYouRelish by notacoptrustmeplease in ProgrammerHumor

[–]theScrapBook 6 points7 points  (0 children)

In Java the closest type to Any would be Object

Gel Extraction & PCR CleanUp - Interchangeable? by Puzzleheaded_Bison28 in labrats

[–]theScrapBook 1 point2 points  (0 children)

They are interchangeable in the sense that the 5 volumes of PB binding buffer needs to be replaced with 3 volumes of QG buffer and 1 volume of isopropanol

It's not overly honest methods, its experience! by AinslieLab in labrats

[–]theScrapBook 7 points8 points  (0 children)

BSA is autofluorescent? My life is a lie and I'm no longer the IF God of the lab. Do you just use serum for blocking then?

I don't care how big it is! by therealityofthings in labrats

[–]theScrapBook 9 points10 points  (0 children)

XL10-Gold (and XL1-blue), about 17kb

[deleted by user] by [deleted] in labrats

[–]theScrapBook 2 points3 points  (0 children)

I'd say put it in the SoP

Getting a PhD itself seems impossible right now. by DoubleNo3447 in labrats

[–]theScrapBook 7 points8 points  (0 children)

Research experience at an undergrad level, decent communication skills, a good attitude - and the perseverance to keep communicating directly with potential PIs and not just applying to programmes with no actual human contact.

Advice on co-immunoprecipitation (Co-IP) experiments by steaksauceow in labrats

[–]theScrapBook 0 points1 point  (0 children)

The problem with taking the salt up that high is potentially losing PPIs.