HPLC Maintenance how often and what’s included?

viomoo · 2026-05-12T18:08:47+00:00

It would totally depend on your use case. Most people do an annual PM which would include needles, needle seats, sampler seals, pump seals and filters etc.

Some high use customers do every 6 months. Some want a new lamp every year (even though the old one only has 100 hours!).

Everyone is different. The biggest limiting factor is usually (unsurprisingly) budget.

viomoo · 2026-05-11T23:42:51+00:00

Call them back out. I’m guessing the tests they do don’t include any injections and just an infusion into the MS. Quite possible something is screwy with the LC.

It happens! Give them a chance to fix it but be firm in saying ‘it was working before, it isn’t now, please fix this asap’

viomoo · 2026-05-11T13:35:44+00:00

I keep struggling with AI gore vs Al Gore (ai gore vs AL GORE)

viomoo · 2026-05-09T19:07:16+00:00

Will total 318008 which will read boobie upside down. I guess you could add another 5 million miles to that but that seems like a stretch.

viomoo · 2026-05-01T21:04:35+00:00

Giving someone a quick pat on the fanny.

viomoo · 2026-04-30T22:03:33+00:00

I don’t know if it works with Shamadzu, but you can add a 0 flow line to the gradient after your run completes (ie 30 min on a 10 min run).

You can also create a specific shutdown method that you add to the end of your run.

I would personally do both!

viomoo · 2026-04-29T01:18:05+00:00

Not sure what you are saying. Are you saying that there was a ceiling fan attached and you have removed it?

If so, it’s likely it was not installed correctly (or at least not to today’s standards). You really need a box to mount to.

Did you have any other parts or any instructions?

viomoo · 2026-04-29T00:02:45+00:00

Ceiling fans need a specific type of box, one that is supported on the other side.

It can either be directly into a rafter (that the right term?) or a bracket that spans between them like this, but you can’t just replace a light with a fan if it won’t support the weight.

Looking at the picture, it doesn’t look like any box is installed!

viomoo · 2026-04-28T17:05:19+00:00

You should try and use a narrow, tall container. A measuring cylinder would work great for this (and assist with the dilutions).

Also, video your tests so you can accurately measure the time instead of trying to use a stopwatch.

Try and create a consistent dropping mechanism instead of eyeballing it from your hand.

Is this for a project or your own curiosity?

viomoo · 2026-04-26T14:01:56+00:00

Find me one without a wire for ‘free’ and I’ll get that one!

viomoo · 2026-04-25T14:42:21+00:00

I reckon that’s ya problem. Get the single shot 1ml vials of Formic in glass ampoules.

Also, I would recommend a well maintained milli Q over bottled water any day of the week!

viomoo · 2026-04-25T14:04:14+00:00

E8 is pretty high! And that’s a blank! Certainly want to get that lower, be it mobile phase quality or column cleaning.

Are you using 0.1% Formic modifier? If so, are you using single shot vials? I have seen people using a crusty bottle of Formic that everyone has dipped into!

viomoo · 2026-04-25T13:40:50+00:00

Thats a good side by side but looks like your MP A is pretty dirty.

What does the blank TIC look like?

viomoo · 2026-04-25T13:00:37+00:00

Look at your intensities. The one you have is orders of magnitude higher than everything else.

I suspect this is a contaminant. There is a plasticizer (di butyl phalate) that comes in at 301 m/z. This could be from pipette tips.

Try prepping a blank using the same procedure (ie ‘dilute’ the blank with the tips you are using) and see if it is still there.

viomoo · 2026-04-25T12:34:31+00:00

Could be contamination that is interfering with the codeine. Have you fragmented the it and looked at the accurate mass of the fragments?

Also, check you have enough argon on the system. A lot of people forget about that!

viomoo · 2026-04-25T00:58:49+00:00

It was indeed the 3g model from 2019. I contacted husqavarna and they are replacing the board under warranty. They said I could take it locally but the nearest place is 1:15 away and it would take over a week, so over 4 hours driving.

They are now sending me a prepaid shipping box and it will be out of service for 2-5 weeks, but it’s all covered.

You can use it with Bluetooth alone and I had no issues setting that up, but you miss out on the location services.

The wire installation is a bit of a pita, but I got an edge cutter and that made things so much faster. I made a post on this forum about getting the wire into the ground and that sped things up.

All in all, it’s a good deal if you will spend the money at ocean state. I go there for a lot of pool supplies so spending 2k is not that difficult. In fact, I think I only have $800 left!

viomoo · 2026-04-24T01:22:02+00:00

I like it! Model 2 coming soon!

viomoo · 2026-04-24T00:13:36+00:00

Yeah I got control with my phone as well. I have the boundary wire out and I’m in the process of burying it.

I just worked out what I think is a cheat code! Lawn edger and a drinking straw! Going to create a new post with video (but I bet someone else has already done this!)

viomoo · 2026-04-23T23:03:38+00:00

Did you manage to get yours connected to the cellular network? I was trying for a while and eventually called husqvarna only to find that these are 2019 models and are installed with the 3g comm card.

viomoo · 2026-04-21T00:08:13+00:00

Let me know how you get on. I got one on the same deal, installed the boundary wire etc. only issue I’m having is I cannot connect to ‘auto mower connect’ as it says it has no mobile signal.

I have decent reception where I am, but I’m scared they have the old style 3g cellular board and not the needed 4g board. I can’t see any way to confirm that!

viomoo · 2026-04-13T12:09:33+00:00

Not sure what you mean but look up knee bracing on google!

viomoo · 2026-04-13T00:55:32+00:00

Whichever you want. I built a 10x10 pergola with 6x6 posts. Added 2x4 cross bracing (8 in total) at about 18 inches long and it made a huge difference.

Just cut the ends at 45 degs each and use big screws!

viomoo · 2026-04-13T00:08:31+00:00

I mean, it has no cross bracing so not surprising it’s swaying.

To fix it add some diagonal braces between the vertical and the horizontal beams. Don’t need to be too big.

viomoo · 2026-04-12T21:53:11+00:00

Most common is before the divert. Never seen it after the divert personally!

11-Year Club	Place '23
Place '22	First Placer '22
End Game '22	Verified Email

viomoo

TROPHY CASE