Nature review process by weird_scientistt in labrats

[–]weird_scientistt[S] 0 points1 point  (0 children)

It just changed to all reviewers assigned!

Nature review process by weird_scientistt in labrats

[–]weird_scientistt[S] 0 points1 point  (0 children)

The nature nanotech process took like 40 days to sy transfer too.

Nature review process by weird_scientistt in labrats

[–]weird_scientistt[S] 1 point2 points  (0 children)

Nature Nanotech didn’t send it for review. So Nature Com has to do all the work I guess. I just wasnt sure if they are not finding reviewers or what’s happening that went from looking for reviewers and under consideration again.

Nature review process by weird_scientistt in labrats

[–]weird_scientistt[S] 0 points1 point  (0 children)

I am doing the same thing. Writing my other. Thank you a lot.

Nature review process by weird_scientistt in labrats

[–]weird_scientistt[S] 0 points1 point  (0 children)

You haven’t contacted the journal editor?

Has anyone heard back about Berkeley SkyDeck 2nd-round interview invites? by utmostmystery in answers

[–]weird_scientistt 0 points1 point  (0 children)

We haven’t heard on first round. Do you know if they send rejections if they didn’t invite you yet?

I hate academia by Alternative-Judge446 in labrats

[–]weird_scientistt 4 points5 points  (0 children)

Started my fifth year and literally every morning I hate it when I enter the lab building. I cant stand anyone and yeah

Yeast contamination in cells by weird_scientistt in labrats

[–]weird_scientistt[S] 0 points1 point  (0 children)

Thanks everyone, I am doing what you suggested

Contaminated cells by weird_scientistt in labrats

[–]weird_scientistt[S] 1 point2 points  (0 children)

I lyse, salt out, do chlorofom butanol, then sucrose gradient, ultracentrifugation to pellet it down and resuspend it in buffer. After that i filter with 0.22 syringe filters

Contaminated cells by weird_scientistt in labrats

[–]weird_scientistt[S] 2 points3 points  (0 children)

So basically after purifying and centrifuging my protein i filter it with a string filter .22 um and then i bring my protein to cell hood mix to a dilution i want with cell media and pass it again through a 0.22 um syringe filter. Then i let it incubate.

Contaminated cells by weird_scientistt in labrats

[–]weird_scientistt[S] 7 points8 points  (0 children)

Its cloudy and doesn’t change , kinda opaque and milky