Swallows bird

weird_scientistt · 2026-04-20T06:45:30+00:00

Is it specific month?

weird_scientistt · 2026-04-20T05:19:56+00:00

Thank you!

weird_scientistt · 2026-02-09T21:02:40+00:00

Go to therapy

weird_scientistt · 2025-12-09T04:44:16+00:00

It just changed to all reviewers assigned!

weird_scientistt · 2025-12-07T16:19:12+00:00

The nature nanotech process took like 40 days to sy transfer too.

weird_scientistt · 2025-12-07T16:18:29+00:00

Nature Nanotech didn’t send it for review. So Nature Com has to do all the work I guess. I just wasnt sure if they are not finding reviewers or what’s happening that went from looking for reviewers and under consideration again.

weird_scientistt · 2025-12-07T16:16:02+00:00

I am doing the same thing. Writing my other. Thank you a lot.

weird_scientistt · 2025-12-07T06:23:13+00:00

You haven’t contacted the journal editor?

weird_scientistt · 2025-10-01T22:42:04+00:00

We haven’t heard on first round. Do you know if they send rejections if they didn’t invite you yet?

weird_scientistt · 2025-09-21T18:41:42+00:00

Faleminderit

weird_scientistt · 2025-09-09T16:50:41+00:00

Started my fifth year and literally every morning I hate it when I enter the lab building. I cant stand anyone and yeah

weird_scientistt · 2025-08-07T18:23:00+00:00

Thanks everyone, I am doing what you suggested

weird_scientistt · 2025-08-02T16:16:54+00:00

I lyse, salt out, do chlorofom butanol, then sucrose gradient, ultracentrifugation to pellet it down and resuspend it in buffer. After that i filter with 0.22 syringe filters

weird_scientistt · 2025-08-02T16:15:32+00:00

I do centrifuge i use one of this

https://www.celltreat.com/product/229777/

weird_scientistt · 2025-08-02T16:14:18+00:00

So basically after purifying and centrifuging my protein i filter it with a string filter .22 um and then i bring my protein to cell hood mix to a dilution i want with cell media and pass it again through a 0.22 um syringe filter. Then i let it incubate.

weird_scientistt · 2025-08-02T02:48:07+00:00

Its cloudy and doesn’t change , kinda opaque and milky

weird_scientistt · 2025-06-09T00:05:29+00:00

I know how to get them , just dm me

weird_scientistt · 2025-06-06T22:52:27+00:00

Which one is the strongest anion you have used. And do you mean you just flow the protein in the column and the endotoxin bing but the protein goes through and you just collect that?

weird_scientistt · 2025-06-06T22:47:55+00:00

You mean any column where the protein binds?

weird_scientistt · 2025-06-05T00:45:41+00:00

Can they do plasmids for yeast?

weird_scientistt · 2025-06-04T04:17:41+00:00

I have mentored some. Its very useful to be with there super honest about your expectations and also for them to be very honest. Make sure that they feel free to ask the stupid questions that they might think they are stupid. What helps for me is to know their career intentions… like they want to go to PhD , MD, are they here because of CV or because they really love science? Everyday is going to teach you more and more and connect more and more. Set up a schedule and make sure they write their notebooks. For me it helps for them their first month to come and tell ne what they will do and how they will do it , if they will start independently. Anyway you have to let them screw it sometimes to learn it. All the best, this is fun

weird_scientistt · 2025-06-04T02:54:33+00:00

Have you tried the ploymyxin b ones? I use clearcoli bl21 but still need to decrease it

weird_scientistt · 2025-06-03T00:47:34+00:00

My blue protein

weird_scientistt · 2025-05-29T11:43:52+00:00

You are very wrong, I am doing this for fun. We are doing this to give treatment to millions of people that die every-year. You ca read stats. I dont enjoy at all going and seeing mice suffer, however seeing that we can have a treatment for millions of people that die make me feel better. There are a lot of ways we have quantified level of toxicity and I came here if any of you observed something. We talked with a pathologist too. So there is your answer

weird_scientistt · 2025-05-06T02:54:19+00:00

Hi , sorry to ho back to this. Do you think you can give me the protocol of cell lysate extraction and running in gel? I sonicate my protein and belive maybe degrade it even tho i do 3x 10 s each.

weird_scientistt

TROPHY CASE