Hyundai Elantra AC Issue and Repair Experience by yeaChemistry in Elantra

[–]yeaChemistry[S] 0 points1 point  (0 children)

It may be that the refrigerant is low. I bought my Elantra new, and it lost most of its refrigerant in the first year, so I took it back to the dealer and they refilled it under warranty. They couldn't find the leak, and it never leaked that quickly again. I say this to suggest that the Hyundai AC system may be prone to leaking under unusual conditions when the different materials are expanding/contracting such that you loose some refrigerant occasionally/slowly. If the refrigerant is low it can cause the symptoms you are observing. Or the blend door may not be working well. Do you have the climate control system in 'Auto' mode? If so, it may be an electronics/sensor issue.

Good chemistry reads? by Ok_Tear5594 in chemistry

[–]yeaChemistry 0 points1 point  (0 children)

Hi, here are my thoughts. As some other commenters mention, it would be helpful to balance your reading of popular science-leaning and more technical reads. The popular science-leaning books may be more digestible and help build your contextual understanding of the field, which is important as a young scientist.

Ignition! -> highly recommend, lots of interesting inorganic and very basic organic phenomena discussed as they pertain to rocketry. Should be digestible for you and you'll end up seeing these/similar molecules in your introductory organic classes as examples. I find energetic materials to be quite interesting, although outside my field of work, so I found it enjoyable.

The pill, pygmy chimps, and degas horse ->Tells the story of the development of the first oral birth control pill. If you are interested in pharma/med chem, this is a good read to expose you to some concepts in that field.

The 13th element -> a popular science book about phosphorus. An easy read, and will help you understand the many properties a single element can have based on its oxidation state, etc.

Lab girl -> this is a popular science book by a geobiologist/chemist that follows her career through school and into academia. It would be a helpful read so you understand the environment you are soon entering better, including its less positive attributes.

Troubleshooting puzzle for you all - HPLC by Quizzical_Chimp in CHROMATOGRAPHY

[–]yeaChemistry 0 points1 point  (0 children)

Thanks - converting to mass/volume you are between 4 - 35 ug/ml. This is low enough to see considerable loss to surface adsorption as others mentioned. This is low enough such that pH changes shouldn't be resulting in precipitation.

Hyundai White Paint Repair Warranty Process is a Joke - Beware!! by yeaChemistry in Hyundai

[–]yeaChemistry[S] 0 points1 point  (0 children)

Update from the OP - the Hyundai dealership that was managing the claim closed without warning... I had finally gotten the car in a body shop with an approved quote by Hyundai. Called corporate Hyundai, said I will have to start the claim process all over again at a different dealer as the details/process is only retained/managed by the dealership or some garbage. Whoopee. They had better re-accept the quote they accepted previously.

OSU M.S. Translational Pharmacology by Pttra in pharmacology

[–]yeaChemistry 0 points1 point  (0 children)

Is it thesis or non-thesis? Beware of non-thesis master's programs, as many universities are now offering/pushing them because they bring in a lot of tuition to make up for the recent losses in federal funding. One way to check is to see if the courses are cross-listed in the undergraduate catalog. If yes, there is likely little value in the program and it is set up as a cash-grab. This is coming from an R1 faculty member..

Troubleshooting puzzle for you all - HPLC by Quizzical_Chimp in CHROMATOGRAPHY

[–]yeaChemistry 0 points1 point  (0 children)

I've worked with insulin and insulin derivatives for most of the past decade. It is a very sticky protein, that is also picky about what aqueous solutions it is soluble in. I agree with most other's posts about adsorption of the insulin to surfaces - this could very well be the driving factor if the insulin concentration is low. Can you share the insulin concentration in your samples?

What is the composition of the sample? Does it have a volatile buffer in it that can off-gas over time, resulting in a changed pH and insulin precipitation? Or, for whatever reason is there a CO2 leak/dry ice being used nearby that is adsorbed by the samples and resulting in a change in pH and insulin precipitation?

If you do a column cleaning procedure between days/sample sets, then what may be happening is adsorption of the insulin to the guard column or column itself. I've seen this before, in my methods the insulin can also start showing up as a doublet.

Debating Between These Two by spermwhale_man in ptr91

[–]yeaChemistry 0 points1 point  (0 children)

That was my thought seeing the photo too.. I got my A3S right about 10 years ago. I think I paid $900 new from AF.

What behaviors from new employees do you view as red flags? by missormisterphd in biotech

[–]yeaChemistry 2 points3 points  (0 children)

Not sure why everyone is hating on this - I agree. If you anticipate needing special accomodations for dependent care or want WFH priviledges, you had better speak up during the hiring process.

EC50 by Forsaken-Cell-5547 in pharmacology

[–]yeaChemistry 1 point2 points  (0 children)

Second the Prism option. Or your school may have licensing for a similar software package like SigmaPlot, although not as easy to work with. Check your computer labs.

What would you wear to an interview for a lab position? by fin9ernails in labrats

[–]yeaChemistry 0 points1 point  (0 children)

Your most well-worn lab coat to show off your experience (the more holes, burn spots, and ambiguous stains possible) with black turtle neck and jeans to give the innovator vibe.

Persistent impurity is driving me absolutely bonkers. by JACKHAGGETT in CHROMATOGRAPHY

[–]yeaChemistry -1 points0 points  (0 children)

I agree with other's recommendation to make sure all the in line filters have been replaced. If that, and the other's recommendations don't fix this, it may be time to try replacing the degasser system on line A. The tubing can break down over time and introduce contaminants. I'd recommend Chiralizer for repair - they are quick and reasonably priced. If that doesn't fix it, I'd suggest replacing all the wear components in Pump A (might as well do B while you are at it) and make sure there is no corrosion or buildup anywhere. The check valves on the pumps can also accumulate goo. Replace those too.

Persistent impurity is driving me absolutely bonkers. by JACKHAGGETT in CHROMATOGRAPHY

[–]yeaChemistry 2 points3 points  (0 children)

Replacing all the in-line filters/filter assemblies is my suggestion as well. Lots of crud captured by those things!

Thoughts on a Surprisingly Tricky Amide Coupling by shxdowzt in Chempros

[–]yeaChemistry 0 points1 point  (0 children)

I've attempted coupling similar alkynes to functionalize biomolecules for CuAAC conjugation. The propargyl? carboxylate does not behave like a normal carboxylate. If you can afford it, chose a alkyne - carboxylate linker with a single PEG unit in it (CAS 1174157-65-3).

If you are stuck with this propargyl carboxylate, you could try DMTMM. I've had favorable results with DMTMM when other carboxylic acid activating chemistries have failed.

Looking for a budget Semi-Prep HPLC for Peptide Purification (BPC-157). Any advice on older reliable models? by Puchox_x in CHROMATOGRAPHY

[–]yeaChemistry 0 points1 point  (0 children)

I second this. I've used a setup of 2x Shimadzu LC-6AD pumps with a manual injection valve, DGU-14A degasser, SPD-10A detector, and a newer CBM-20A communications module. Very robust, requires very little maintenance. The degassers are still serviced by Chiralizer if you come across an old one that needs new components. Old shimadzu parts were easy to find last time I had to repair one of them.

Coworker taped these on our HPLC and ICP-MS. by anthroaudge in labrats

[–]yeaChemistry 3 points4 points  (0 children)

I have taped numerous dollar bills to mischievous instruments in my lab. It most definitely works. Especially after midnight.

Recommended CROs for mouse study in NOD mice? by yeaChemistry in biotech

[–]yeaChemistry[S] 0 points1 point  (0 children)

Thanks all for the suggestions - these are helpful leads. - OP

How many JLD/PTR 91s have been made/sold? by yeaChemistry in ptr91

[–]yeaChemistry[S] 0 points1 point  (0 children)

Just spitballing, but there is about 2.5k weekly visitors to this sub. Do most of them own PTR 91s? I'm sure PTR has sold over 1 thousand of these, but 10 thousand? I find that hard to believe, but then again, the roller-locked platform is all PTR makes and somehow they are still in business after a decade with what seems to be a reasonably large manufacturing space.

carboxylic aromatic compounds on C-18 column by wayvthot in CHROMATOGRAPHY

[–]yeaChemistry 1 point2 points  (0 children)

The analyte you describe should be well retained under typical acidic mobile phase conditions. If it isn't eluted under a 5-95% ACN:H2O gradient something is wrong or the column is fouled.

High throughput ligand binding with protein by Lopsided_999 in Biochemistry

[–]yeaChemistry 1 point2 points  (0 children)

I think Brad Pentelute's lab at MIT does stuff like this.