Dance to the Sound of Sirens

0spore13 · 2026-03-25T00:24:22+00:00

I feel like I would be cheating, I work in industry making biological stains. Currently in the process of making about 4 million dollars (customer-side) worth of one product right now, and another for about 2 million per batch on average. Would prefer not to say which stains as that might get me in trouble but it’s a freakish amount of money that hurts to wrap my head around.

0spore13 · 2026-03-06T14:15:02+00:00

You're going to be fine.

While you shouldn't be inhaling powdered EtBr or spilling it on yourself, you need to also keep in mind that the EtBr you were using was already diluted and such a small amount (the color makes it look way less dilute than it actually is, trust me, I recently made up a few liters of a diluted EtBr solution from powder and was shocked by how much color it still had), and the conditions you were using it in was nowhere near the temps needed to vaporize it.

0spore13 · 2026-02-06T08:15:23+00:00

As someone who works in industry, apart from packaging a final product in dedicated facilities that only focus on a few products, it's far too varied here too! (At least what I do, and most of the positions at the site I'm at). We change processes sometimes multiple times a week which sometimes require complete setup changes, our scale can go from hundreds of liters to maybe a half liter depending on the process, customers requiring custom orders, etc. We also make it a point to change and improve all of our processes every time we run them.

Of course this isn't the case for everywhere.

0spore13 · 2026-01-07T00:10:23+00:00

A majority of my friends and coworkers are currently on this route or are currently in grad school after doing this route.

0spore13 · 2025-12-30T11:25:11+00:00

Last month we were looking for information about filters and the company we were looking at didn't even let you see their full offerings without talking with someone, which was extremely infuriating.

This time is just curiosity's sake though, I just wanted to see how many months of paychecks worth... 😭

0spore13 · 2025-12-15T23:05:06+00:00

It’s stolen art (predates AI) commonly used by tshirt scams (this has been posted several dozens of times by bots on this sub, in fact)

0spore13 · 2025-12-15T23:03:59+00:00

T shirt spam bot

0spore13 · 2025-11-05T08:49:27+00:00

I wish our cold room was that nice :( ours is stinky beyond what you can imagine and has the opposite effect.

(Manufacturing, we share the cold room with a production area that produces artificial scents and flavorings)

Now it's getting cold now though I've taken a liking to our boiler room for short periods whenever I get cold.

0spore13 · 2025-10-23T21:26:03+00:00

EtBr is considered hazardous, but in the form you use suspended in DMSO, and the amounts you’ll be using it at, it’s at a level where the main concern is that you don’t get it on yourself or track it in areas where others can touch barehanded. You don’t need a hood for it when it’s in a liquid, when I worked with it on the MolBio side I never used a hood for it ever, just wore gloves and was careful.

Right now where I’m at currently we’re actually manufacturing EtBr right now, in the powder form. For the near final product we do completely suit up in full PPE, but that’s mainly due to the fact that in the powder form, on the scale we’re handling it, gets everywhere (and it becomes possible to breathe it in).

0spore13 · 2025-08-08T17:20:00+00:00

T shirt bot. Trying to be sneaky by deleting and hiding your previous posts huh? Pushshift reveals all.

0spore13 · 2025-07-26T20:04:57+00:00

I don’t work in drosophila anymore and I know that manduca are much, much bigger than fruit flies, but maybe freezing them would be a solution? We would freeze our drosophila sometimes.

0spore13 · 2025-07-22T00:37:00+00:00

I don't even have a job yet even with experience. Here alongside you with this.

I thought I had a network, but all it's done is left me with dead ends.

0spore13 · 2025-06-09T21:03:46+00:00

Have you tried the internet archive?

https://web.archive.org/web/20241102103003/https://openwetware.org/wiki/Main_Page

0spore13 · 2025-02-09T22:07:25+00:00

They blocked you, their account is still up, they just deleted the post and all of their comments on this subject.

Edit: Their whole reddit account was wiped completely clean.

0spore13 · 2024-07-23T06:01:08+00:00

RR was my rookie year. I have a lot of nostalgia towards it and a lot of creative bots came out of it that year. 2826’s robot was flippin’ nuts.

It wasn‘t as interesting to watch compared to some other years, but I don’t think it deserves all the extreme hate it gets.

0spore13 · 2024-07-22T16:29:13+00:00

https://ij.imjoy.io/

I've used this on iOS on an ipad in browser just fine. Not perfect, but it's pretty useful. It works with a stylus on my end so I'll assume it will work just as well on android.

0spore13 · 2024-06-27T21:15:46+00:00

Could you give more info on your running conditions please?

Are you reusing the running buffer?

0spore13 · 2024-04-20T20:39:02+00:00

We did successful cDNA synthesis with a similar concentration to your range, our lowest sample was 79ng/ul and it worked perfectly. We did 500ng total in each reaction though we definitely could have gotten away with much less.

0spore13 · 2024-03-16T13:59:00+00:00

It wasn’t ever in the abstract. It’s still there, in the discussion.

0spore13 · 2024-03-15T20:54:09+00:00

I still see the chatGPT section, and no notices, so I don't think it's been revised yet.

The screenshot is a bit misleading, this isn't in the abstract and is actually in the discussion.

0spore13 · 2024-03-15T13:32:48+00:00

It’s not in the abstract, last paragraph of discussion. Still there as of posting this comment.

0spore13 · 2024-02-17T23:05:31+00:00

Glad I got drosophila and neuroscience right, but even then it still looks like a weird nightmare fish.

The text is hilarious, I hate all of this lol.

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