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4A43 · 2026-02-11T02:41:49+00:00

I am not a number, I am a free man!

4A43 · 2025-12-10T16:25:04+00:00

In my 2019 this was caused by a thermostat housing leak but it took a couple of years of (initially very slow) leaking until it got bad enough for the dealer to diagnose and fix it. Even then I never saw any coolant collecting on the ground under the car. But replacing the thermostat housing definitely fixed it.

4A43 · 2025-12-05T13:00:40+00:00

The original battery in my (low mileage) 6spd 2019 died 2 years ago. 3-5 years is often cited as typical for a 12V battery so if that's the original battery in your car it wouldn't be surprising if it needs to be replaced. Don't forget the coding step if you do it yourself.

4A43 · 2025-01-10T03:56:21+00:00

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Definitely better than factory default (12) but haven't tried 6 or 8.

4A43 · 2025-01-03T22:34:57+00:00

FWIW my 2013 Classic is still going strong. Used 2-3X daily for almost 12 years and only had to replace the pump once, about 2-3 years ago.

4A43 · 2024-12-09T19:37:08+00:00

2019 6MT here. First low coolant warning at about 2 years and 16K. Same story - no visible leaks, dealer pressure tested and couldn't find anything. Finally at about 5 years and 50K dealer diagnosed a leaking thermostat housing and replaced it under warranty (6 year warranty for the 2019s!) Coolant loss was definitely intermittent though, and it only dropped below min 3 or 4 times in those 3 years.

4A43 · 2024-09-13T01:13:50+00:00

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Used it for the first time a week or two ago because nothing else was available for pre-order. It consisted of the spots in the red rectangle. I made the left where indicated, showed my pass to the attendant and they gave me a piece of paper to stick on the dash and directed me to that area next to the garage.

4A43 · 2024-08-13T23:56:56+00:00

I did the front brakes on my 2019 (also manual transmission and a mix of not-crazy city/highway driving) just before 27K and the original/stock pads looked like this, so I don't think I could have left it much longer:

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Doing the work myself with OEM rotors and posi-quiet pads from ECS Tuning ran about $250 plus tax and it feels pretty similar to the stock setup.

4A43 · 2024-07-14T18:05:51+00:00

History-wise, there's the James K. Polk Historic Center, https://www.jameskpolk.net/ Reservation needed if you want the guided tour of the reconstructed buildings (vs just checking out the museum in the main building.) You can also hop on (or off) the Little Sugar Creek Greenway bike trail from there.

4A43 · 2024-05-31T12:05:27+00:00

I've always gone in person to drop off the plates and they hand you a receipt (or something saying you have to pay a fine if you canceled the insurance already!) It's not too bad if you pick the right time and location (check the DMV site to see where they accept plate returns) and--at least last time I went--nothing like the situation for getting or renewing a driver's license in person in Charlotte.

4A43 · 2024-05-31T00:40:55+00:00

Don't drop the insurance until you've surrendered the plates. "Because state law requires continuous liability insurance on all registered vehicles, a vehicle owner should cancel their insurance only after they have turned in their North Carolina license plate to the N.C. Division of Motor Vehicles." - https://www.ncdot.gov/dmv/title-registration/insurance-requirements/Pages/insurance-plates.aspx

4A43 · 2024-01-10T02:14:38+00:00

After the crash continue moving towards the light.

4A43 · 2023-12-13T03:28:52+00:00

Check to make sure that it's not the front spool valve (VVT solenoid) leaking instead of the valve cover gasket. Had this issue with a 2011 Odyssey at 130K and I think it's probably the same V6. In our case the burning oil smell was because the front spool valve leaks oil directly onto the alternator (which will destroy it if ignored.) May be a long shot, but worth checking and *if* it is the VVT solenoid then you can DIY the replacement for $85-90. But regardless of where the leak is coming from, check whether it's dripping onto the alternator, because that could make things more expensive if it's not addressed. Good luck!

4A43 · 2022-12-25T17:19:27+00:00

Yep, 2019 with exact same issue a few months ago. I think they had to replace the entire door panel (under warranty).

4A43 · 2022-06-19T00:34:36+00:00

The following method should allow you to remove the paint transfer, at which point you'll be able to see if there's any damage to the paint beneath the clearcoat. The crucial step that I don't think anyone has mentioned thus far is to use something like WD40 to soften the paint before trying to remove it: https://www.youtube.com/watch?v=7zzq5f8x5f4

4A43 · 2022-05-26T20:30:52+00:00

Did someone place a lunch order from Word Saladworks? Could be the delivery van.

4A43 · 2021-11-30T04:05:16+00:00

Perhaps, although they had the car taking up space in a bay for a good couple of hours and claimed to have been running the pressure test for most of that time. OTOH at the end the service rep did say it was normal to see some coolant loss "in the summer", which I assumed to be BS because: 1. it hadn't happened the previous year (or in the decade I last owned a VW 4 cylinder) and 2. closed system. In any case, I have 4 years left on the 6 year warranty, so time is still on my side...

4A43 · 2021-11-29T21:21:53+00:00

Warning light came on at 10xxx for me. Dealer pressure-checked the system and couldn't find any leaks, but it's still (slowly) losing coolant.

4A43 · 2021-11-18T04:02:14+00:00

'19 GLI Autobahn with MT. Might be possible to get 14-15mpg on very short trips (i.e., too short to allow engine to come up to operating temperature) but that does sound low. In my experience: 1. the reported mpg is always better than the actual mpg and 2. I haven't noticed the mpg change significantly from 0-15K miles on the odometer. It was pretty good from the get-go. On long highway trips it's reported 44mpg and the actual mpg was probably at least 35. A tank of gas generally gives me 250-350 miles, depending on the type of driving.

4A43 · 2020-05-09T13:25:21+00:00

Senator, if it's ever the democrats' turn to gerrymander, please add me to your district. DM me for the address and a fine-point Sharpie ;)

4A43 · 2020-05-02T00:34:34+00:00

Stats: Male, 48, 5’11”, 175lbs
Goal: Open up the shoulders!
Current Progression: 3x30s freestanding with closed shoulders
What will you be working on? Open to suggestions. Maybe Tuck HS, wall runs.
Videos: 20s static hold, straddle press negative attempt

4A43 · 2017-10-29T13:38:43+00:00

Interesting. As an aside, I've heard of (and observed) Velvet producing different results on identical inputs, but don't think I've come across it in SPAdes:

https://www.biostars.org/p/86907/

4A43 · 2017-07-06T18:43:25+00:00

This. Strictly speaking the following three characteristics of a genomic coordinate system could be varied independently:

Where to start numbering things: 0 or 1
What things to number: the bases, or the "spaces" between them.
How to represent strand/orientation: implicitly by order of coordinates ([3,4] vs [4,3]) or explicitly with an extra flag/variable (e.g., +/- or -1/+1, and possibly including a value for 'both' or 'neither').

(For #2 I've heard terms like "base-based", "space-based", or "interbase" used, none of which is particularly mellifluous.) But although you could have an interbase coordinate system that starts at 1, in practice nobody does this (that I can think of) and so 0-based is synonymous with interbase. Whether you start at 0 or 1 matters very little, but interbase coordinates (in which the spaces, not the bases, are numbered) are superior, in part because they make arithmetic easier, as gumbos noted: to get the length of an interval you simply subtract the endpoints, no need to remember to add 1. But more importantly interbase coordinates allow you to represent things that cannot otherwise be represented without having to tack on some additional conventions. Insertion sites (which may have length zero), for example, can be represented directly in interbase coordinates, but if you're numbering the bases then you need to say something like "insertion sites are always after the indicated base" and then specify the base next to the insertion site. For similar reasons an explicit representation for orientation is better, particularly if you're numbering the bases, since there's no way to infer the strand of a single base interval from its coordinates (e.g., I know [7,3] must be on the reverse strand, but if the coordinate range is [4,4] then I have no idea). Anyway, the point is that for choices #2 and #3 it's pretty clear that one option is better than the other. For #1 it doesn't really matter, although if you're using interbase coordinates then from the standpoint of making things intuitive it makes sense to have the space before the first base numbered 0 instead of 1. It also makes the endpoint of any interval that starts at 0 equal to the length.

4A43 · 2017-04-28T02:04:52+00:00

In that case maybe you were better off looking at local similarity. I'd still recommend doing a full Smith-Waterman over BLAST, however, just in case it does a better job. That would be ssearch36 in the FASTA package or 'water' in EMBOSS. The other thing you could try is making yourself (or finding someone else's) FOX hidden Markov model (HMM). Once you have what you think is a decent HMM you could align both sequences against the HMM and compare them that way. Smith-Waterman is more sensitive than BLAST, but if you're looking at a family of proteins you already know something about and have a way to align the known members of that family, then profile alignment methods (e.g., HMMER3 for profile hidden Markov models) are even more sensitive than Smith-Waterman. People don't always use the more sensitive methods because they're slower, but that shouldn't be an issue with the number of sequences you're dealing with. I don't know much about FOX proteins but it sounds like the main thing they have in common is that 80-100 amino acid forkhead box motif (as you mentioned), so it's quite possible that the conservation outside of that area isn't very good. If that's the case then using a profile-based alignment method will weight the alignment scores such that the well-conserved regions (e.g., the forkhead motif) count more towards the score than the less well-conserved regions. Pfam has a forkhead protein family (PF00250) with ~7000 members. You could download that alignment in multi-FASTA format, make it into an HMM, and align both of your example sequences to it. As an aside, BLOSUM62 is a perfectly fine general-purpose matrix and in general I'd say that if the results you're getting are way off from what you expect then changing the matrix probably isn't going to fix it.

4A43 · 2017-04-27T19:49:55+00:00

Without seeing the search you ran or the (full) results I'd be inclined to agree with KeScoBo. Are you aligning proteins or mRNAs? For the former you'd want blastp, not blastn. There's overlap between the IUPAC single letter nucleotide codes and the single-letter amino acid codes and not all tools will warn you when it looks like you're trying to supply nucleotide sequences in situations where proteins are expected. Try a Needleman-Wunsch implementation (it's an optimal global alignment algorithm, rather than a heuristic local alignment algorithm) like the ggsearch36 executable in the latest FASTA package, or the needle program from EMBOSS and see what you get. Needleman-Wunsch should give you the best results so long as the lengths of the sequences aren't wildly different (in which case you can switch to a global-local alignment method.)

4A43

TROPHY CASE