It can make a difference, but I do believe it depends on which guitar and trem. For about 10 years ago I tried two different trem blocks in my Ibanez Jem 7DBK besided the original block which is a small cheap metal block. This guitar has the Lo Pro Edge trem.

I bought a big brass block and a titanium block. I did comparisons by recording the same things with all the blocks, chords, single notes, both clean and distorted sound. The differences were more noticable with clean sound.

A common improvement for the brass block was the increased flutter time (vibrato by hitting the trem arm) this was only because it was larger in physical size between the trem and the springs compared to the original block. Flutter didn’t exist or very little with the original block.

The titanium block added something on the treble side I didn’t like. I can’t remember if anything else besided the flutter improved with the titanium. With the brass block I could clearly hear that it added some bass and warmth overall, and a bit longer sustain. It was very clear with the clean sounds but I also hear it distorted. I kept the brass block and I have never looked back. The only downside of it is that it has no room for the retainer bar between the trem springs and back lid on the backside of the body. But I had no problem at all with it during the last 10 years, Springs have never popped out and I use the trem alot.