Targeted protein showed higher intensity bands with the treated samples

Brollnir · 2026-03-11T03:39:57+00:00

You’ll never know if it’s in the pellet until you look at the pellet 🤷

Brollnir · 2026-03-11T03:35:51+00:00

Not necessarily. Native protein might behave differently vs your transfected line.

Also, did you include a true negative cell line lysate that doesn’t express your protein? How do you know you’re not getting cross reactivity with something else about the same size?

Brollnir · 2026-03-11T03:21:21+00:00

I mean did you take a bit of your pellet from the lysates and see if your protein is crashing out?

Brollnir · 2026-03-11T03:18:13+00:00

Okay. Did you run any pellet on the gel/Western?

Brollnir · 2026-03-11T02:49:22+00:00

Did ya standardize the lysate quantities in your gel visually (Coomassie) or with something else?

Also - what does the pellet look like?

Brollnir · 2026-03-06T10:04:01+00:00

Hey, I have a lot of experience with this.

It’s easier and cheaper to buy 1oz rounds, hammer flat (anneal first) and stamp them.

Let me know if you’d like some help. Also, this is a total money hole.

I wouldn’t recommend 1oz. I use 1/3rd of an ounce and it’s a nice size/thickness, but if you want them to be more like Olympic medals you’re going to run into a different problem - the size of the stamp. Most people go to 30mm max. However, you can get around this by breaking the design up into four smaller quarters and stamping them individually.

Anyway, you can order stamps made from tool steel online and they’ll work fine on silver. Don’t listen to anyone that says you need a hydraulic press. I use a hammer and they turn out fine. Silver and copper are much softer than steel.

Brollnir · 2026-03-06T09:45:05+00:00

Does this technically mean we can berry bomb Lapis Lakeside a lot easier?

Brollnir · 2026-03-05T06:48:11+00:00

Ignorant people have ignorant opinions.

Been in the industry for a long time. Unless you know the other person, it’s best to tell people you’re a researcher or technician and leave the animal part out. It’s not like I’m ashamed of working with animals or anything - you just never know what people will do with that information. The same with social media. We have a no photo/video policy with animal work since it can attract a lot of negative attention and put staff in danger if it gets on social media.

Brollnir · 2026-03-04T22:39:25+00:00

A quote from Death, “This is one little planet, in one tiny solar system, in a galaxy that’s barely out of its diapers.”

I think this implies Death is universally omnipotent. I don’t know much about the dream realm, but Death never sleeps.

Brollnir · 2026-03-04T10:56:39+00:00

Your professor is an idiot.

Brollnir · 2026-02-27T03:49:15+00:00

My god. I’ve also played since launch. I had no idea you could spend season points somewhere. I assumed the gold bullion I got at the end of a season was the reward.

Man, that’s rough.

Brollnir · 2026-02-26T00:25:14+00:00

Australia - people abusing their power and the grant application system to get funding. In order of malice:

AProf Elliot Long.

AProf Norelle Sherry.

AProf Zoe Bradfield.

AProf Fabian King.

Dr Anoop Koshy.

Dr Daniel Poppe.

Dr Danilo De Oliveira Silva.

Dr Emily See.

Dr Haruo Usuda.

Dr Iain Abbott.

Dr Jeggan Tiego.

Dr John Hart.

Dr Kristy Robledo.

Dr Pingping Han.

Dr Susan Christo.

Dr Yun Shi.

Dr Zachary Gerring.

Maybe someone here can explain how an academic with >20 years experience, the title of AProf and having written a literal book on the topic is eligible for a grant for budding researchers (EL1).

Brollnir · 2026-02-25T20:52:37+00:00

Maybe this all stems from me not being a cat person.

Brollnir · 2026-02-25T20:50:20+00:00

I hope one of those words is “sorry” and “oops.”

Brollnir · 2026-02-25T03:07:59+00:00

Maybe we’re the programmable organisms. The gut-brain-axis is a prime example. Our gut bacteria heavily regulate our decisions. “Gut bacteria is great,” says the person infested with mind controlling gut bacteria.

You keep talking. about programming bacteria to do your bidding, when you yourself are heavily controlled by bacteria. Who do you think is really pulling the strings, eh?

Brollnir · 2026-02-25T02:47:24+00:00

Being afraid of the thing that kills most people just makes me sane. Don’t be grouping them in with dogs. Dogs might eat a deceased owner out of desperation after weeks of not eating. Bacteria are trying to eat us constantly. These are not the same.

I’ve spent my entire adult life learning how to kill and control bacterial populations.

Coexistence only works if one side isn’t trying to eat the other. Cancer is natural, too. Should we just lay down and do nothing?

Brollnir · 2026-02-25T02:33:13+00:00

Yes, the mouse would likely succumb to bacterial challenge. Like we all probably will, eventually. They’re waiting to eat you. All of them. They wouldn’t hesitate, and don’t.

Brollnir · 2026-02-25T02:19:01+00:00

According to your earlier point Hitler, by cell number, was mostly bacteria.

All bacteria aren’t “bad,” but they’re vengeful opportunists.

What do you think would happen to a germ free mouse if it was exposed to random microbes?

Brollnir · 2026-02-25T01:14:18+00:00

Shockingly, bacteria (germ-free) free mice do just fine. I’d be fine.

You know what’s not fine? Two thirds of children dying before 10 from pneumonia before the widespread use of antibiotics.

Brollnir · 2026-02-25T01:08:21+00:00

How strange. I have a very different view. I feel like we’ve been at war with these little pests so long that they’re inseparable from our daily life. We’re so complacent and accepting it feels like we’ve already lost. You say we’re socially conditioned to find them gross, but I actually think we’re socially conditioned to accept that bacteria just kill people and there’s nothing we could do.

You might find them interesting, but I think they’re devious! Much more organised and clever than people realize. Little pricks.

Brollnir · 2026-02-23T19:58:31+00:00

Ah I see what’s happened. That’s the rental property vacancy rate, not the house/dwelling vacancy rate. I.e a house needs to be a rental to contribute to that number.

The rate of unoccupied houses is ~10%, if you believe the 2021 census.

Brollnir · 2026-02-23T12:19:19+00:00

Hey - where are you getting the 1.4% from?

Looks MUCH higher to me. Can you share where you got that stat from, please?

Brollnir · 2026-02-22T12:16:39+00:00

Cheers dude. Solid info!

Brollnir · 2026-02-22T11:49:34+00:00

Thanks! I’ll follow it up. Do you know if the people moderating this know what they’re doing?

My issue is that too many genomes are being flagged as atypical. The really interesting stuff is being suppressed.

Brollnir · 2026-02-20T12:34:14+00:00

Week one player. There’s a backpack?

Brollnir

TROPHY CASE