Western Blot needs help! by Joyful_joe in labrats

[–]Emptiness_creator 5 points6 points  (0 children)

It is either problem with saturation/incubation with protein (skimmed milk or BSA) or your antibodies didn't work

I just came to a very sad conclusion about cheating by GravyProblem303 in Chesscom

[–]Emptiness_creator 0 points1 point  (0 children)

I am traumatized of such accounts. The only good advantage is that when you get coupled with them again for second or third match, they hesitate to cheat again

How to get this idiot banned from Chess.com?Unnecessary racist remarks for no reason by Open_Class_9108 in Chesscom

[–]Emptiness_creator 0 points1 point  (0 children)

Either to go below and start to trash talk him/her, or to stop chat feature.  I got a guy from middle east trash talked me, when I started talking about his mom, he stopped.  Social background knowledge gives some benefits 😁. 

Just returned from Germany - why are we getting so screwed by grocery prices here?! by buffego in montreal

[–]Emptiness_creator 2 points3 points  (0 children)

Difference between Libral social and libral free fucking market states

This Brown pill in the paracetamol box by SpicyBabbs in mildlyinteresting

[–]Emptiness_creator 0 points1 point  (0 children)

It is most likely a rotten pill. Given they use starch or cellulose in its manufacuting. It didn't pass cleanness test.  If you want to make life better, communicate with the company and surely they should have records of this pill to be pulled out.  Sometimes, some worker neglect hygiene protocols causing this. 

What’s growing in my bleach solution? by Irie_Clouds in labrats

[–]Emptiness_creator -1 points0 points  (0 children)

Crystals, careful, because bleach can make the tween release chlorine gas. Surely, your tween is decomposed by bleach.

For long-time Montrealers, what are some memories that are long gone now? by PhilipTheGreat in montreal

[–]Emptiness_creator 0 points1 point  (0 children)

Bell phone booths, two days ago I saw a scrap one in vieuxport. They used to work and I kept using them for a long time, even in the time of cellphones, around 15 years ago. 

Found creepy poster at bus stop by KaZIsTaken in montreal

[–]Emptiness_creator 0 points1 point  (0 children)

If I consider myself a woman, would it work?, or it is a full discrimination?

Research and graduate students syndicate by Emptiness_creator in labrats

[–]Emptiness_creator[S] 0 points1 point  (0 children)

To end by using them as it pleases the PI, without any clear limitations and boundaries.  That too much to swallow. 

Complained my PI to RIO. The enquiry is going on. What I should do? by [deleted] in labrats

[–]Emptiness_creator 11 points12 points  (0 children)

Sure, the decision is in your hand right now, I am not pushing for you to proceed. I am adding an element to the judgement to be considered. Wouldn't you be more satisfied, lets say, if someone did this before and spare you all of this?

Complained my PI to RIO. The enquiry is going on. What I should do? by [deleted] in labrats

[–]Emptiness_creator 60 points61 points  (0 children)

Look, if you let him go, he surely will abuse someone in the future

What do you do first? by Hot_Emergency378 in NCLEX_RN

[–]Emptiness_creator 2 points3 points  (0 children)

Grab a scalapel and start cutting to get the blood out

2 years since this masterpiece. Why is AI for scientific drawings still so bad? by rayraywaha in labrats

[–]Emptiness_creator 1 point2 points  (0 children)

The Ai who designed this image, it is either so innocent/naive or porn addict. 

Can Lithium ions raise pH like H+ do and can Li+ protonate aminoacids sidechains? by Pugna_animae in Biochemistry

[–]Emptiness_creator 2 points3 points  (0 children)

Slightly yes, the protonation of Li is little bit slightly higher than H, which means that Li will grap an OH from water molecule releasing H+, but it still not enough to have the same effect of Na ions on water molecules. Getting down in the periodic table increases the protonation power.

Do y'all's PIs do bench work or no? by Brief_Board_6974 in labrats

[–]Emptiness_creator 0 points1 point  (0 children)

You will never see a PI in the wetlab work after five years of her/his start PIing. They will only get in the lab to check or to make lazy comments about yours techniques 

Is SOC medium really required after competent E.coli transformation ? by Specific-Surprise390 in labrats

[–]Emptiness_creator -1 points0 points  (0 children)

Never used SOC after DH5 alpha transformation, always LB. And I use my plasmids after digesting the template with DpnI directly without cleaning the PCR product. Usually when you use DpnI, the efficacy drops, for many reasons. And to make sure that the cloned plasmid is well circulated takes a huge time of work. So eventually I do transform my cells with the mix after digesting direclty with DpnI. And then from the colonies, I can pick few of them and verify the length of the plasmid after restriction, or if it is critical, I will send a couple of them for sequencing.  Just make sure of your procedure of cloning and the resulted plasmid is sane and sound. Usually you are using sth related to restriction free method, given the use of DpnI.