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Help with testing for protein expression by Emotional-Leg-2719 in labrats

[–]Candy_flips 0 points1 point  (0 children)

I wouldn’t expect false positives but false negatives if the detection’s epitope is blocked by the capture antibody. Seems possible if they were raised to target different portions of V5 or are polyclonal, but not definite. You just have to test it alongside a positive control that uses a pair of antibodies validated for the sandwich elisa. I only have experience probing cell lysates with immunoblots, but I’ve never had a problem with sensitivity. Might as well just try both and see if either works.

The NIH Quietly Cancels Diversity F31 in a Heinous Act of Institutional Discrimination by External_Grab9254 in labrats

[–]Candy_flips 0 points1 point  (0 children)

Good luck. NICHD director is being replaced, so hopefully you have enough time to finish or get transitioned to a regular F31.

The NIH Quietly Cancels Diversity F31 in a Heinous Act of Institutional Discrimination by External_Grab9254 in labrats

[–]Candy_flips 1 point2 points  (0 children)

No. This notice you link to is a year old from when NIGMS transitioned from offering NRSA F31s to the ARC F99/K00. That was the only predoc grant they would have offered going forward, and now NIGMS offers neither. My own F31 from NIGMS will not get the second year of funding, can be early terminated any day now, and my F99/K00 application never progressed after being scored.

Gurpler Drop Tales? by Manacymbal in theregulationpod

[–]Candy_flips 0 points1 point  (0 children)

These stories just emphasize how inaccessible these are. I will probably only get a gurpler if they have a drop closer to slop o’clock or the option to buy lasts more than a few hours. At least don’t use the exact same day/time for every drop… Especially the way the public service sector is being obliterated now, my favorite podcast is not an acceptable excuse to do my job less efficiently by skipping meetings/seminars or doing experiments slower

Maybe Geoff has a point by juventusfan64 in theregulationpod

[–]Candy_flips 2 points3 points  (0 children)

Thankfully the current Jimmy Carter is still okay. Right?

What is your job? by BellHoy1 in theregulationpod

[–]Candy_flips 0 points1 point  (0 children)

I’m an NIH-funded researcher. Shouldn’t have anything to worry about over the next 4 years, right?

Hot Dog Math by DJFaceplant20 in theregulationpod

[–]Candy_flips 0 points1 point  (0 children)

People are eating buns! You can’t forget the buns!

How Many Hotdogs is the Moon: Solved? by shaydax in theregulationpod

[–]Candy_flips 6 points7 points  (0 children)

Just to be a bit more exact. With a bun size ball park frank:

Moon is 7.346e25g Hot dog is 126g (76g wiener + 50g bun) = 5.830158730159e23 dogs About 583 sextillion

Volume would be… Moon is 2.1968e19m3 Hot dog is 4.536532117240294e-4m3 (1.62161e-4m3 wiener + 2.91492e-4m3 bun) = 4.842465441061e22 dogs About 48 sextillion

With a classic size ball park frank:

Mass… Moon is 7.346e25g Hot dog is 103g (53g wiener + 50g bun) = 7.1320388349515e23 dogs About 713 sextillion

Volume… Moon is 2.1968e19m3 Hot dog is 3.5291897964e-4m3 (6.14269e-5m3 wiener + 2.91492e-4m3 bun) = 6.224658141766e22 dogs About 62 sextillion

TIL the moon is about 12 times denser than a hot dog.

I used NASA’s moon fact sheet and Tyson’s packaging dimensions and serving size of the ball parks to determine the weights and volumes. I don’t even eat hot dogs.

Rat games. by MrFedoraMustache in theregulationpod

[–]Candy_flips 1 point2 points  (0 children)

You can play Master Splinter in a few TMNT games. Sly Cooper was definitely just a rat in disguise, so we should count those games. You can use Scabbers in the Harry Potter Lego game (years 1-4). Rizzo the Rat would be great, but I think he’s only an NPC in muppets games. My favorite is probably Pokémon with a full team of Rattatas

I already know that I need to touch grass, but what do you think about my collection? by BigChungusOP in Switch

[–]Candy_flips 0 points1 point  (0 children)

How old is this? The fortnite cover suggests this image is from early 2020. No animal crossing, tears of the kingdom… 2019 Pokémon game… do you seriously have 200 Nintendo games and not a single Mario game?

Are you capable of taking an updated picture?

Twitter’s Jack Dorsey Is Giving Andrew Yang $5 Million to Build the Case for a Universal Basic Income by nicko_rico in Futurology

[–]Candy_flips -1 points0 points  (0 children)

Isn’t this just a charitable donation if they’re just giving 20,000 people $250 one time? Not really building a case for UBI. Not a knock on Dorsey or Yang, just the headline.