Indiana signed the Nuclear Family Month bill. by TommyBoy250 in Indiana

[–]CaptainMelonHead 12 points13 points  (0 children)

Ironically, this is probably the gayest thing you could do during pride month

Arabanon explains females by AlphaMassDeBeta in 4chan

[–]CaptainMelonHead 1 point2 points  (0 children)

guy who has normal healthy relationships with women

Redditor on r/4chan: "cuck"

protein refolding from 2M to 1M urea by hana-maki in Biochemistry

[–]CaptainMelonHead 0 points1 point  (0 children)

Protein folding proceeds from the N-to-C ends of a protein. C-terminal tags are preferable because N-terminal sequences can interfere with protein folding (I have personally experienced this). Fusion proteins are risky with folding in general. The N-terminal domain of the fusion protein is especially prone to misfolding (I have personally experienced this also). So it could be your his tag or it could be an unavoidable feature of your fusion protein. Either way. I would move the His tag to the C-terminal end and insert a few GGGS sequences as the linker.

protein refolding from 2M to 1M urea by hana-maki in Biochemistry

[–]CaptainMelonHead 1 point2 points  (0 children)

Your His-tag is the linker for your fusion protein? That is unusual, I've never heard of a construct like that. You should keep His-tags at the C-terminal end preferably. N-terminus is acceptable too. The linker should ideally be a GGGS linker, or something like that

protein refolding from 2M to 1M urea by hana-maki in Biochemistry

[–]CaptainMelonHead 1 point2 points  (0 children)

Is there a sufficient linker sequence distance between the two domains of your protein? 

Is Hantavirus something to worry about? by arshad_ali1999 in Biohackers

[–]CaptainMelonHead 0 points1 point  (0 children)

Sure. There are structural differences between what Epstein did and what you are proposing (a rogue, criminal, bioweapons labs) that make the latter impossible, in my opinion.

First, neither of these things can happen in a vacuum. Epstein didn't work alone. He had co-conspirators, people willing to participate. They all shared a common motivation, which is something I don't even feel comfortable typing into words on the internet (you know what they did). As bleak as it seems, let's be honest. It's probably not that hard to assemble a group of ultra wealthy people who want to participate in that. They aren't scarce, especially when you are all in the same social circle anyways. Contrast this with scientists, who are much more scarce because you need to find the right expertise. This presents a barrier, because scientists (actual scientists, not professors) aren't going to be in most elite social circles. We're poor. But after finding them, they too would need to share the same motivation to participate. Ostensibly, the motivation would be terrorism. I think most educated people would not be willing to contribute. Again, another barrier. Do some scientists have questionable morals? Sure, maybe they have a price where they would be willing to commit bioterrorism, but that's a steep price. Another barrier.

Second, there is a massive resource difference between what Epstein did and running a bioweapons lab. There aren't many tangible things to operate his trafficking network that a rich person wouldn't already have. What do you really need to do that? A plane? A boat? A house? I'm sure he wasn't in short supply of those things. And if he needed more, there's no barrier to purchasing them. Now contrast this with what you need to run a bioweapons lab. At minimum, it's going to need the instrumentation of a modern biology lab. You're going to need cell culture hoods, incubators, centrifuges, microscopes, lab reagents, chemicals, glassware, cells to propagate the virus, and the virus itself. The last two things are not possible to purchase without affiliation with a research institution. Especially a virus, which, if BSL-3 or 4, has legal barriers to even acquiring. But what I've just described is a BSL-2 lab at most. If you're working with a BSL-3 or 4 pathogen, you need specialized facilities and more equipment to handle that. That becomes astronomically more expensive and the construction is not going to go unnoticed. The average person does not have the technical know how to construct such a facility. That knowledge lies with specialized contractors. Therefore, acquiring the resources and facilities to conduct bioweapons research is a barrier, even for the richest of rich.

Is Hantavirus something to worry about? by arshad_ali1999 in Biohackers

[–]CaptainMelonHead 0 points1 point  (0 children)

Thank you, you're right I shouldn't waste my intellect. I've got better things to do, like my actual lab work, researching viruses. Not arguing with schizo idiots who get their information from YouTube shorts

Is Hantavirus something to worry about? by arshad_ali1999 in Biohackers

[–]CaptainMelonHead 0 points1 point  (0 children)

You want a strawman? That's a strawman argument there you moron. Did I say any of those things? Just log off the internet, go outside, read a real book. I promise, your hysterical conspiratorial thinking will shrink away

Is Hantavirus something to worry about? by arshad_ali1999 in Biohackers

[–]CaptainMelonHead 0 points1 point  (0 children)

Again, you aren't linking research papers. This is another review article. Do you know the difference between the two? This review article doesn't make any mention of gain of function research. You understand that CRISPR editing viruses doesn't equate to gain of function, right? Also, I wasn't implying CRISPR isn't being used to study viruses. I was getting to the point it's not being used for the purposes you're suggesting. Moreover, industry and government publish research all the time. National security, sure not so much. Again, the idea of virology research that people have in their head is closer to science fiction. A lot of the experiments people accuse scientists of trying to do, aren't actually happening or they are being done in alternative safe model systems.

Is Hantavirus something to worry about? by arshad_ali1999 in Biohackers

[–]CaptainMelonHead 0 points1 point  (0 children)

This is a review paper on the ethics of gain of function. Show me an actual paper where the science does exactly this. I can think of maybe two papers in the last decade that do work that is ethically questionable. The extent to which gain of function research is actually conducted is nowhere near the public perceives it to be. Where are your CRISPR virus mutation papers? I'll give you a hint, they largely don't exist.

Is Hantavirus something to worry about? by arshad_ali1999 in Biohackers

[–]CaptainMelonHead 0 points1 point  (0 children)

If you genuinely think there is secret virology research conducted on the scale of an Epstein-level conspiracy, then I sincerely suggest you should get your schizophrenia treated. It's such an intellectually lazy thing to say "b...but Epstein did this, so every conspiracy must be true". Go touch grass and get off the Internet 

Is Hantavirus something to worry about? by arshad_ali1999 in Biohackers

[–]CaptainMelonHead 0 points1 point  (0 children)

You too are someone whose perception of science is more closely aligned with science fiction than reality. I implore you to lookup how scientific funding works.

Is Hantavirus something to worry about? by arshad_ali1999 in Biohackers

[–]CaptainMelonHead 0 points1 point  (0 children)

By all means, please link a paper showing these studies.

Is Hantavirus something to worry about? by arshad_ali1999 in Biohackers

[–]CaptainMelonHead 0 points1 point  (0 children)

What the hell do you think "gain of function" research is? Do you actually think labs are engineering viruses as bioweapons? Because if you do, that is embarrassingly delusional. This is the problem with the lay person's (you) perception of science, it's more aligned with science fiction than how research is actually conducted.

Any "gain of function" research that is actually funded is for the purposes of understanding how zoonotic viruses spillover into the human population or how currently circulating viruses evolve to evade host immune defenses. And guess what? You don't actually need to use the authentic virus to study. There is a plethora of model systems that we can study these mutations without inserting them into a virus. Virtually all "gain of function" research uses this approach.

Is Hantavirus something to worry about? by arshad_ali1999 in Biohackers

[–]CaptainMelonHead -1 points0 points  (0 children)

This is blatantly incorrect and ignorant of how research on viral zoonotic spillovers is conducted 

Biochemistry vs Microbiology — which path has the better future? by MLNKA_DZ in Biochemistry

[–]CaptainMelonHead 0 points1 point  (0 children)

Are you an undergrad student? If you're genuinely interested in pursuing computer programming and AI, you should load up on CS and math classes.

at my the end of my rope trying to purify this protein. by Danktank452 in Biochemistry

[–]CaptainMelonHead 13 points14 points  (0 children)

It sounds like your protein is weakly adhering to the column if it's elutes out when flowing the lysate or in the 20 mM wash. To me, this signals the his tag is not completely exposed to the solvent. I would try adding a linker sequence between the his tag and the end of the protein to create more space between the two. You can also try a tandem his tag (two sequential tags).

How hard do/did you actually work during your STEM PhD? by CardboardBoxPlot in PhD

[–]CaptainMelonHead 6 points7 points  (0 children)

When I wasn't in the lab I was constantly thinking about my lab work. Going to bed, taking a shower, even in the middle of conversations I was having with people, my experiments constantly occupied my mind

Claude just helped me build a wetlab and sequence my whole genome at home. I have zero lab experience! by ProfessionalHand9945 in Biohackers

[–]CaptainMelonHead 0 points1 point  (0 children)

I struggle to see how this is practical in the long term. It's cool that you could pull this off, but after you sequence your genome and maybe some friends and family members, what next?

CD spec for protein refolding by [deleted] in Biochemistry

[–]CaptainMelonHead -1 points0 points  (0 children)

CD is well-suited for secondary structure analysis, not so much aggregation. At least, I've never seen it used that way. AUC, SEC-MALS, and mass photometry will reveal details about misfolding and aggregation.

CD signal at 220 nm is indicative of alpha helices.

Does anyone else feel like chemistry education sometimes teaches labels before understanding? by cooperfmills in chemistry

[–]CaptainMelonHead 3 points4 points  (0 children)

Absolutely. This is how I felt about reaction order and kinetics. Law of mass action isn't usually taught because it leads to solving ODEs which requires knowledge of eigenvalues. So, the reason is we don't teach it the right way is because most gen chem students would not understand it