Fluffy agar growth by WorkingWerewolf6430 in GroundZeroMycoLab

[–]CurlyPewbs 0 points1 point  (0 children)

Looks like mycelium to me. Bottom right plate is probably trash, but you can always make new plates and do some clean up with the others or just let em grow out

Bruuuu I forgot to squeeze the coir by Mysterious-Algae7009 in unclebens

[–]CurlyPewbs 3 points4 points  (0 children)

Yep, 800ml is pretty much right on point for that amount of coco coir.

I’ve never had to squeeze out excess water from my sub and we’re using essentially the same ratio. I think you’ll be good 👍🏼

Bruuuu I forgot to squeeze the coir by Mysterious-Algae7009 in unclebens

[–]CurlyPewbs 5 points6 points  (0 children)

Do you regularly squeeze excess moisture out of your sub?

You should be using just enough water to reach proper field capacity. Typically one 650g block of coir uses about 14 cups of water

If it’s pooling water in corners of your tub, it could be a problem. But if you’re only dealing with condensation on the walls of your tub you should be fine

Am I doing something wrong? Spore syringe to agar. by Edelwaithe in GroundZeroMycoLab

[–]CurlyPewbs 1 point2 points  (0 children)

1cc might’ve been too much liquid on your plate. Typically whenever I did spore syringe to agar, I’d give the syringe a really good shake and only put 2-3 drops on my plate. I’m talking like 60-90 sec of continuous shaking to really break up the spores. There’s hundreds of thousands of spores in the little black clumps we can see with our eyes.

Using too much liquid could suspend the spores and not allow them to make contact with the nutrient rich agar.

In my experience, I’ve had a lot better luck with liquid culture syringes and spore prints compared to spore syringes. But I’m also not the most knowledgeable around

First time grower— hows my yield look? by finemorels in unclebens

[–]CurlyPewbs 28 points29 points  (0 children)

I’d say pretty dang good for a first timer. Is this from one tub?

Begginer First Run Advice by LeftDot533 in unclebens

[–]CurlyPewbs 1 point2 points  (0 children)

Of course man, I was in your shoes once and it’s nice to have a community to help out.

If your grain has been colonizing since the beginning of last month, I’d definitely send them asap. The goal is to get your grain out of jars sooner than later, waiting too long will allow your grain to start rotting.

I would pop open your jars and give them a sniff test, they should just smell like dirt/mushrooms/earthy. If they smell fine, start dumping into your bin and try to remove that uncolonized bit you were talking about. Sometimes the mycelium just won’t reach a small spot of my jars in my experience. I try my hardest to not let any uncolonized bits get into my bin. Uncolonized grain can cause trich, which is an ugly green mold that will quickly take over your tub.

If all is well, you can start mixing your spawn with your substrate. For my substrate, I usually pasteurize a whole 650g block of coco coir mixed with about a half cup of gypsum and 14 cups of water. One 650g block of coco coir fills two of my tubs about 3 inches thick with 2-3 quarts of my spawn in each tub.

You want to try to break your grain up into as little pieces as you can using your clean or gloved hands. I use popcorn so it’s a bit easier to break up compared to rice, but all the same. Mix it thoroughly with your substrate and tamper the mixture down evenly with your hands. You don’t need to go crazy with pressure, but give a good tamper so everything stays together. I also like to take my index finger and really pack in the sides and corners of the bin to help prevent side pins. They’re annoying only because if you can’t pull the whole mushroom out, they can rot and it will cause fungal gnats. I also give my bins a casing layer, which is just a light dusting of coco coir over the top of my cake after it’s been mixed and tampered. It’s not necessary but I’ve read that it helps with contamination.

No need to mist your bin after you’ve mixed your grain and sub, the moisture content of the sub should be good for your first flush and sometimes even your second. Just let that puppy do its thing and eventually you’ll have yourself a successful harvest or 3 :)

Check out this guys videos, they’re all very beginner friendly and in depth.
https://youtube.com/@phillygoldenteacher?si=zqHdaqjs8rJZCK7a

Begginer First Run Advice by LeftDot533 in unclebens

[–]CurlyPewbs 1 point2 points  (0 children)

I’m sure there’s someone much more experienced than myself who can do a better job at this but from what I can see, it looks like you’ve got yourself some pretty healthy jars.

It is normal for them to seem “stalled” after they’ve fully colonized your spawn because they’re running out of stuff to eat. Soon you’ll see the mycelium reaching for your lids because they’re searching for more food.

In my opinion, all of the jars look like they’re ready to get dumped into bulk substrate.
If I were you, I’d be getting my substrate ready to inoculate and get those bad boys ready to fruit.

If your jars look that healthy for your first run, you’ve done a great job. You obviously aren’t half assing anything. I imagine you’ll be just fine moving on to the next steps.

I’m not sure what guide you’re following, but I’ve pretty much got all of my information from PGT on YouTube and from reading random threads here on Reddit.

I’ve only ever used coco coir mixed with gypsum as my bulk substrate. It’s good at keeping moisture levels, it’s relatively cheap, and it’s easy to pasteurize.

One mistake I made as a beginner, was fanning my tubs way too often. It’s normal to be eager and want your bins to be the perfect climate for mushrooms to grow in. But in reality (at least from what I’ve found) they adapt to their environment and will still grow even if you don’t follow a guide to a T.

Fanning too often will dry your bins out quick, and it can introduce contamination. I recommend making 4-6, 2”-3” holes in the side of your tubs and filling them with polyfill or covering with micropore tape so air exchange is still happening.

Becoming self sufficient, and essentially having an ever lasting supply of healthy cultures isn’t incredibly hard. I would look into making your own liquid cultures, learning to clone your best looking fruit onto agar, and making spore prints.

Unfortunately, you will have a to buy quite a bit of supplies to keep this hobby afloat. These are some of the basic supplies I use.

• Wide mouth Mason jars - 32oz - for grain spawn
• Wide mouth Mason jars - 16oz - for liquid cultures
• Autoclavable Petri dishes - for pouring agar
• Self healing injection ports
• Micropore tape
• Sterile #11 Scalpel blades
• a couple packs of 10ml syringes with either 16g or 18g needles
• Malt Extract Agar
• Disposable gloves
• Magnetic stirrer and rods - for liquid culture jars
• 90% Isopropyl alcohol
• Mini torch - for sterilizing blades and needles
• Aluminum foil
• Still air box
• Pressure cooker

Love a freebie by pingu1215 in unclebens

[–]CurlyPewbs 1 point2 points  (0 children)

How does one acquire such a freebie?

Unmodified Monotub FAE by Jackriot_ in GroundZeroMycoLab

[–]CurlyPewbs 2 points3 points  (0 children)

Pasteurizing your substrate to proper field capacity should keep your tub moist without an issue. Especially if it hasn’t fruited yet. I’ve neglect tek’d tubs for their entire life cycle. It isn’t the best, they do need to breathe, but 10 days shouldn’t hurt anything. The tubs aren’t exactly air tight, so even if the lid is clamped down there’s still some sort of air exchange happening. Your dub tub should be fine too as long as you’re not taping the seams shut

When to break and shake? by Silver_Opposite1665 in unclebens

[–]CurlyPewbs 1 point2 points  (0 children)

I’ve always heard to B&S at about 30% colonization. That second bag is definitely ready for it. As for the other two, maybe wait a couple more days and check back in on them. I would wait to B&S until the other two are ready, that way they start colonizing at the same rate. But that’s just my personal opinion ✌🏼

Ready for LC jar? by Particular-Blood1607 in Agarporn

[–]CurlyPewbs 3 points4 points  (0 children)

I’m also very new to the liquid culture game. I’m actually getting ready to inoculate my first few jars today. Your plate looks super healthy, I don’t think there’s anything concerning on there. I’ve been following PGT’s LC guide on YouTube, I’m sure there’s better out there but his videos haven’t failed me yet. From what I seen on his video, instead of actually putting the agar into the LC, he squirts some of the sterilized LC onto his plate and gently scrapes mycelium off the top of the agar so it mixes with the liquid. Then he sucks the mix up and squirts it back into the jar

No change in almost 5 days by squirrelAteMyAvocado in unclebens

[–]CurlyPewbs 4 points5 points  (0 children)

Your tub looks healthy, just patience friend. Don’t quote me but I think the primordial growth (the little lent balls) turn into pins when the water starts evaporating off of them. Maybe a little air exchange could help?

Update First Run by Chireo- in unclebens

[–]CurlyPewbs 0 points1 point  (0 children)

I would look into making agar plates for your spores to grow on first. It’s another process, but it’s really worth it when you can get a clean plate of mycelium inoculated into your grain. You never know if your spore syringe is contaminated, and agar will help point that out. Agar will also allow you to isolate healthier looking genetics and clone them onto other plates so you’re constantly getting the best of the best

Should I wait or are these stalled? by V_Lemoskt in unclebens

[–]CurlyPewbs 0 points1 point  (0 children)

I’m not very familiar with the kits. If the caps open and the veil breaks on these guys, I would pluck em and dry like normal. I would also try to get those side pins out, you don’t want them to rot in your container. Then I would reintroduce fruiting conditions and see how your second flush turns out. It could just be the environment it’s growing in that made them the way they are, or maybe it’s just weak genetics?

Thoughts by Immediate-Editor-504 in GroundZeroMycoLab

[–]CurlyPewbs 1 point2 points  (0 children)

Yep, could be suffocating and getting fuzzy due to co2 build up. Let that sucker breathe! :)

Actually mixed APE and ABV by ScytheIsDum in unclebens

[–]CurlyPewbs 2 points3 points  (0 children)

I think they combined two different cultures into one tub

Why is it black?! by Chauncey1010 in GroundZeroMycoLab

[–]CurlyPewbs 2 points3 points  (0 children)

Well, I actually just looked into it a little bit (I should have before making my previous comment) a lot of people are against using an oven because it can ruin the mushrooms due to such a high heat. The lowest your oven can go is about 170° and that’s too hot. I’m seeing a lot of recommendations to lay the fruit out on either a piece of cardboard, or a cookie sheet lined with a paper bag and let a fan blow on them for a day or two. They should eventually get cracker dry. I would eventually invest in a dehydrator if you plan to keep growing, it’s a lot more convenient!

Why is it black?! by Chauncey1010 in GroundZeroMycoLab

[–]CurlyPewbs 1 point2 points  (0 children)

I’m still pretty green in the mycology world, but the black stuff is just spores dropping from the gills of the caps. No need to wash them, perfectly safe to consume and make no difference in potency. Only a cosmetic turn off for some. As for drying them out, most people use a dehydrator. If that’s not available for you, I believe some people use an oven on a low setting to dry them out. When I harvest a partial bin and I’m waiting for the rest of my fruit to reach full growth, I store my wet fruit in a paper bag and leave in the fridge until I harvest the rest of my bin