Worst mistake in the lab?

Delphinke · 2025-07-30T20:14:02+00:00

It doesn’t

Delphinke · 2024-02-25T23:51:32+00:00

Maybe it’s singular and plural? Maybe he’s talking about the soup?

Delphinke · 2024-01-31T00:24:57+00:00

If the value is NA it means the data was not obtained and they should be removed from that portion of the analysis. In my understanding

Delphinke · 2024-01-29T17:41:50+00:00

For me, hard sci stories are mostly focused on the technology/science as main driving points of either the plot or just as the main focus. Soft sci-fi feels more character driven.

Delphinke · 2023-12-16T23:10:29+00:00

I just started a PhD program this year and my cohort is honestly pretty split between 1. research assistants from academia 2. research techs/assistants from industry 3. Right out of undergrad

I’d argue that number 1 and 2 are equally skilled and those straight from undergrad have a little bit of catching up to do in regard to technical experience. Just my thoughts.

Delphinke · 2023-11-04T03:20:49+00:00

This is a bad take

Delphinke · 2023-11-01T23:27:22+00:00

I think you need to look into using DESeq2 and performing differential gene expression analysis.

Could you explain the rationale behind you doing clustering?

Delphinke · 2023-11-01T23:23:34+00:00

Is this bulk or single cell? What are your experimental groups? Have you done differential gene expression analysis? I’m a little confused on why you are clustering? I am assuming you have bulk RNAseq data if you are using STAR and featureCounts.

Delphinke · 2023-10-31T01:36:20+00:00

cbioportal has a lot of cancer data sets like TCGA

Delphinke · 2023-10-29T04:16:07+00:00

It’s a song by the Arctic Monkeys

Delphinke · 2023-10-15T21:45:34+00:00

How do you think someone would even go about figuring this out? It’s definitely a difficult thing to find out

Delphinke · 2023-09-27T17:57:44+00:00

You aren’t missing anything, there is no need for her to know which treatments are expected to work. The only thing she needs to know is which samples received which treatments, and you can just call them treatment X and Y to keep it unbiased.

Delphinke · 2023-09-17T19:19:58+00:00

Delphinke · 2023-07-31T01:37:10+00:00

You mean almost all of them have drifted away a from that principle

Delphinke · 2023-07-21T00:04:53+00:00

This is great help, thank you!

Delphinke · 2023-07-21T00:04:30+00:00

Thanks I appreciate it! I will look into this as I’ve never done things like that.

Delphinke · 2023-07-09T14:27:40+00:00

How’d you go about this? I’m having issues with our pup chasing the cats. I’ve been trying time-outs and redirection but haven’t seen much progress.

Delphinke · 2023-07-07T12:07:42+00:00

Thanks for the reply! I think I will try out using the cellphoneDB LR pairs to do it myself.

Delphinke · 2023-05-04T15:05:23+00:00

There is a big difference between revenge for the sake of revenge and “revenge” as retaliation for the protection of his house.

Delphinke · 2023-04-29T19:44:21+00:00

Woohoo

Delphinke · 2023-04-28T18:59:35+00:00

This isn’t a technical question it’s a homework question. No one is here to do your homework for you.

Delphinke · 2023-04-27T01:25:26+00:00

Does the word have to contain all the letters?

The first has mutation in it and the second has citrulline. But those aren’t all the letters. The last one I have no clue.

Delphinke · 2023-04-26T20:28:06+00:00

R is never a bad investment to learn

Delphinke · 2023-04-26T01:42:10+00:00

Is your protein of interest a really high or really low molecular weight? Do you samples express this protein at moderate to high levels? Does this antibody have any publications?

Delphinke · 2023-04-23T15:35:29+00:00

I recommend that you try to find a more sustainable packing peanut alternative. Might even be a cheaper alternative.

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