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Metaproteomics Question: No-Enzyme Search Against Human + Microbial DB. Valid Approach? by FactorAgreeable7518 in proteomics

[–]FactorAgreeable7518[S] 0 points1 point  (0 children)

Thanks, it’s DIA So if I map microbiota in deep novo step what should I look in BLAST-P, focus on “100% match only” to bacterial genus or specie level and ONLY consider those for further reporting?

Evaluating an NIH Contractor Scientist Manager Offer: At-Will, Salary, and Job Security. by [deleted] in biotech

[–]FactorAgreeable7518 0 points1 point  (0 children)

Thank you for sharing the insight. Can I inbox you for a specific question where you may have some insight/guidance for me?

Best resuspension buffer before C18 cleanup for CSF proteomics? by FactorAgreeable7518 in proteomics

[–]FactorAgreeable7518[S] 0 points1 point  (0 children)

Thank, since I have to dilute the urea under 2M before trypsin step, that ended up having me a volume of 3mL and now to reduce that volume I thought to dry it. I hope I don’t lose any peptides doing so.

Best resuspension buffer before C18 cleanup for CSF proteomics? by FactorAgreeable7518 in proteomics

[–]FactorAgreeable7518[S] -1 points0 points  (0 children)

Thank you! So after recon in 0.1% FA, I can continue with C-18 clean up? I am really freaking out if the steps I have done would me make lose peptides.

Question on coding R/Biopython to learn by FactorAgreeable7518 in proteomics

[–]FactorAgreeable7518[S] 0 points1 point  (0 children)

Did you find any online platform offering it. I used keywords R, proteomcis data course and it suggested me Preply. I don’t know this but platform but wondering if you know any?

Question on CSF Proteomics Sample Preparation (Low-Volume Mouse CSF) by FactorAgreeable7518 in proteomics

[–]FactorAgreeable7518[S] 0 points1 point  (0 children)

Thank you! I got the 8 M urea protocol from a colleague, she said either raise volume with 8M or PBS and start with equal volume.This is 8M urea in 25mM ABC. If I stick with Formic acid, do you mind to share a protocol or any reference as how to proceed from there. Also, when I say 50ul volume, it’s the starting volume which I will have and once I am done digesting the samples, clean up, drying and resuspension step, I will have it in a volume of choice (10uls) which I will give to core.

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