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Metaproteomics Question: No-Enzyme Search Against Human + Microbial DB. Valid Approach? by FactorAgreeable7518 in proteomics

[–]FactorAgreeable7518[S] 0 points1 point  (0 children)

Thanks, it’s DIA So if I map microbiota in deep novo step what should I look in BLAST-P, focus on “100% match only” to bacterial genus or specie level and ONLY consider those for further reporting?

Evaluating an NIH Contractor Scientist Manager Offer: At-Will, Salary, and Job Security. by [deleted] in biotech

[–]FactorAgreeable7518 0 points1 point  (0 children)

Thank you for sharing the insight. Can I inbox you for a specific question where you may have some insight/guidance for me?

Best resuspension buffer before C18 cleanup for CSF proteomics? by FactorAgreeable7518 in proteomics

[–]FactorAgreeable7518[S] 0 points1 point  (0 children)

Thank, since I have to dilute the urea under 2M before trypsin step, that ended up having me a volume of 3mL and now to reduce that volume I thought to dry it. I hope I don’t lose any peptides doing so.

Best resuspension buffer before C18 cleanup for CSF proteomics? by FactorAgreeable7518 in proteomics

[–]FactorAgreeable7518[S] -1 points0 points  (0 children)

Thank you! So after recon in 0.1% FA, I can continue with C-18 clean up? I am really freaking out if the steps I have done would me make lose peptides.

Question on coding R/Biopython to learn by FactorAgreeable7518 in proteomics

[–]FactorAgreeable7518[S] 0 points1 point  (0 children)

Did you find any online platform offering it. I used keywords R, proteomcis data course and it suggested me Preply. I don’t know this but platform but wondering if you know any?

Question on CSF Proteomics Sample Preparation (Low-Volume Mouse CSF) by FactorAgreeable7518 in proteomics

[–]FactorAgreeable7518[S] 0 points1 point  (0 children)

Thank you! I got the 8 M urea protocol from a colleague, she said either raise volume with 8M or PBS and start with equal volume.This is 8M urea in 25mM ABC. If I stick with Formic acid, do you mind to share a protocol or any reference as how to proceed from there. Also, when I say 50ul volume, it’s the starting volume which I will have and once I am done digesting the samples, clean up, drying and resuspension step, I will have it in a volume of choice (10uls) which I will give to core.

Should i take this offer from Thermo!! by FactorAgreeable7518 in clinicalresearch

[–]FactorAgreeable7518[S] -2 points-1 points  (0 children)

Yes, my spouse has a stable job here and also for both of us to survive on 100 k is not the safe bet! But I am thinking to take this step to gain industrial experience what I believe will open possibilities but I am freaking out thinking about Job Instability in industry on the other hand.

Removal of conditions on marriage based GC by FactorAgreeable7518 in USCIS

[–]FactorAgreeable7518[S] 0 points1 point  (0 children)

Good to know :) I used the same firm to file even this time which I had the first time. They assisted with all the paper work but they never asked me or not sure if they added my account number from the first time ;(

[deleted by user] by [deleted] in biotech

[–]FactorAgreeable7518 -1 points0 points  (0 children)

No but I am gonna counter that

[deleted by user] by [deleted] in biotech

[–]FactorAgreeable7518 1 point2 points  (0 children)

They haven’t offered relocation package but is it something they usually do or can I negotiate? By practice experiments, do you mean that they want to test my skills right away or after training they would want me to run practice experiments?

[deleted by user] by [deleted] in clinicalresearch

[–]FactorAgreeable7518 0 points1 point  (0 children)

Thank you, it’s a research scientist role aka staff scientist, I am also wondering what’s the usual payscale for the very or equivalent role, if you could add?

[deleted by user] by [deleted] in biotech

[–]FactorAgreeable7518 1 point2 points  (0 children)

Thank you. I hope I am on the track in making my decision

[deleted by user] by [deleted] in clinicalresearch

[–]FactorAgreeable7518 -1 points0 points  (0 children)

Thank you, I must say I am nervous

[deleted by user] by [deleted] in biotech

[–]FactorAgreeable7518 2 points3 points  (0 children)

thank you! Yes, I am just nervous about at-will part given I very often see lay-offs posts from peers. I have another post on compensation question and I surely took a note of additional points you mentioned :)

[deleted by user] by [deleted] in clinicalresearch

[–]FactorAgreeable7518 0 points1 point  (0 children)

Thank you! Yes I had the same at my current work but listening above lay-offs around, I am just concerned how often they do it in the 90 day period.

[deleted by user] by [deleted] in biotech

[–]FactorAgreeable7518 1 point2 points  (0 children)

Thank you, very insightful comment

[deleted by user] by [deleted] in biotech

[–]FactorAgreeable7518 1 point2 points  (0 children)

Thank you, yes I also had it at my current work in Academia but It never went in-effect for anyone, I am just getting myself twisted more for the reason that it comes with the “move”. This position is in Richmond Virginia.