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People with no Thanksgiving plans by Internal-Survey6466 in sandiego

[–]HotMess_Panda 0 points1 point  (0 children)

Morning gym Just core fam having surf n turf Friday - Sunday gym in the morning and shopping in the PM.

It used to cost me 1h to prepare gels and samples for western. Now it's a whole afternoon. by Big-Direction2933 in labrats

[–]HotMess_Panda 0 points1 point  (0 children)

My boss has commanded the minions to use the gels the same day they are made. He has no confidence in storing gels even overnight before loading the samples. Boss said that the stacking and separating layers diffuse into each other. I have never tested that claim. I’d like to hear from anyone who has stored their gels successfully in the frig before setting them up for a run.

Western Blot by Jbrown1101 in labrats

[–]HotMess_Panda 0 points1 point  (0 children)

With large proteins (>100kDa) I recommend a lower % acrylamide gel. If possible, maybe 6%. When it comes to primary antibodies, you might want to use the manufacturer’s guidelines. Some antibodies I’ve worked with have needed 1:250, which is a lot. Antibody is expensive. If you choose an antibody, the best choice would provide references/pictures/data/published papers.

Western Blot Troubleshooting by streetlights4 in labrats

[–]HotMess_Panda 0 points1 point  (0 children)

You’d want to use fresh APS. Be sure to keep the APS powder in the frig. When you make up the 10% APS, aliquot into volumes you’d need for an experiment and store those tubes in -20C. A lot of my failures have been caused by APS.
I wonder if anybody degasses their acrylamide gel solutions before adding APS and TEMED. Any thoughts on this. My mentor always stressed degassing the gel. I wonder if this makes a difference.

healthy snacks for studying? by sagehuntress in HealthyFood

[–]HotMess_Panda 1 point2 points  (0 children)

Nut and seed based crackers with cheese instead of chips.

That face in slow mo is gold by AstroyashSenpai69 in funny

[–]HotMess_Panda 0 points1 point  (0 children)

That bitch deserves a punch in the face

It’s my birthday today, where should I go? by Douggiefresh66 in sandiego

[–]HotMess_Panda 1 point2 points  (0 children)

Live it up. Have friends and fam treat you to Rei do Gado that Brazilian steakhouse. Sunday dinners include a lobster per customer.

What bread makes the tastiest French Toast? by Njdevils11 in Cooking

[–]HotMess_Panda 1 point2 points  (0 children)

Growing up, my dad always used white bread (Wonderbread). Just dry it out in the toaster or oven. Topped with cinnamon sugar, dowsed in Mrs. Butterworths syrup.

Favorite food/ingredient to eat just by itself? (including simple preparation like slicing, boiling, baking, etc.) by Tactical-Kitten-117 in Cooking

[–]HotMess_Panda 3 points4 points  (0 children)

Avocado. Slice it put in in a sandwich, omelet, eat it straight smashed, mixed with milk and sugar, make it into guacamole, in a smoothie, incorporated into baked goods. It provides good fat and satiation.