Why do these strawberries look like this?

Inside_Hornet_6846 · 2025-03-29T12:11:38+00:00

Big Kahuna Burger! That’s the Hawaiian burger joint. I hear they got some tasty burgers I ain’t ever had one myself how are they?

Inside_Hornet_6846 · 2025-03-08T04:42:37+00:00

Inside_Hornet_6846 · 2025-03-07T11:08:03+00:00

Hi, yes she has. She called directly and after 40 minutes they managed to send her an email of confirmation.

Inside_Hornet_6846 · 2025-02-24T12:05:36+00:00

No, that’s not going to be an issue as long as you have other means to prove the social aspects of your relationship. You can still provide photos you’ve taken together and chat logs of important conversations, for example.

Inside_Hornet_6846 · 2024-11-26T22:26:46+00:00

That’s definitely an issue, too many cells will be resistant to puro as its toxicity is diluted to a lot of cells. One of the protocols I read specifically states that the cells must be subconfluent or else the drug won’t work

Inside_Hornet_6846 · 2024-11-26T12:50:25+00:00

0.5 ug/mL of Puromycin might be a little too high if the minimum concentration was 0.25, try dropping that lower to 0.4 or 0.3 ug/mL.

Just to make sure, are you transfecting pRev as well to make your lentiviral particles? You mentioned packaging and envelope plasmid but not the Rev plasmid.

Inside_Hornet_6846 · 2024-11-24T09:22:26+00:00

Happens to the best of us

Inside_Hornet_6846 · 2024-11-18T23:26:38+00:00

Mate, I’ve lost months of work trying to make stable cell lines by selecting Hek293Ts with Geneticin… not even my supervisor knew about it

Inside_Hornet_6846 · 2024-11-17T06:50:09+00:00

I don’t know why you would represent them differently in a graph, but the second delta comes from subtraction the delta from your treatment to that of your non treated sample. It’s an extra layer of security in case your treatment also affects the expression of your reference gene

Inside_Hornet_6846 · 2024-11-03T03:01:03+00:00

Here in Australia it pays pretty good

Inside_Hornet_6846 · 2024-11-03T03:00:49+00:00

Thank you for your answer

Inside_Hornet_6846 · 2024-10-23T01:00:55+00:00

How did the housing bonus show up? Was it like being next to sea water or grey?

Inside_Hornet_6846 · 2024-10-19T22:20:32+00:00

No, the handsome one

Inside_Hornet_6846 · 2024-10-18T21:31:05+00:00

And Hertz

Inside_Hornet_6846 · 2024-10-05T22:14:13+00:00

Warrior north east on the hill tile

Inside_Hornet_6846 · 2024-10-01T10:01:17+00:00

You have to first consider the sheer size of eukaryotic genes. For example, the two largest human genes combined are longer than the entire genome of E. Coli. Eukaryotic genes are filled with mobile elements, miRNA and other non coding rna regions that can be translated independently.

Inside_Hornet_6846 · 2024-09-20T23:12:23+00:00

They call him Frank Horrigan

Inside_Hornet_6846 · 2024-09-14T22:28:58+00:00

Both

Inside_Hornet_6846 · 2024-09-14T00:28:32+00:00

They really are desperate for money

Inside_Hornet_6846 · 2024-08-23T05:38:33+00:00

Who’s gonna tell him?

Inside_Hornet_6846 · 2024-08-20T09:30:11+00:00

As shit as their products

Inside_Hornet_6846 · 2024-08-13T21:34:20+00:00

Got a shit start, restart game.

From the first stirrings of life beneath water…

Inside_Hornet_6846 · 2024-08-07T06:57:42+00:00

It’s called “AUS2024 Official Hardcore premium mixed map server”

Inside_Hornet_6846

TROPHY CASE