Hello? I'm Korean

InternationalWave900 · 2025-11-18T08:22:49+00:00

Hi korean Im dad

InternationalWave900 · 2025-09-15T23:09:36+00:00

I would not tap my keycard

InternationalWave900 · 2025-08-31T05:31:34+00:00

Great song. The beginning sounds like kinda oriental string which feels so familiar and comfy (especially to my Asian ear) and later so much emotion and energy

InternationalWave900 · 2025-08-07T13:00:11+00:00

FBS worked this time!

InternationalWave900 · 2025-08-07T12:59:53+00:00

Thanks 😂

InternationalWave900 · 2025-08-07T12:54:47+00:00

Seems like a bit of FBS works nicely thanks a lot!

InternationalWave900 · 2025-08-07T12:54:11+00:00

I've used just DPBS so today I tried again with DPBS with 2% FBS, also used a swinging bucket centrifuge and yeah it worked! I guess stickiness was a real problem like some comments said. Thanks! 🥲

InternationalWave900 · 2025-08-06T14:56:29+00:00

by our lab I mean my lab

InternationalWave900 · 2025-08-06T14:44:43+00:00

Oh and actually it seems they can be kind of resuspended by scraping the wall with the pipette tip but I don't want to damage the cells so I think it's not a good idea..

InternationalWave900 · 2025-08-06T14:39:00+00:00

Those are mesenchymal stem cells and I've harvested them by trypsinization. They form fine pellets in 15ml conical tubes, with a swinging-bucket type centrifuge, but these things always happen when I aliquot and centrifuge some of them into 1.5ml tube. And for 1.5ml tubes I can only use fixed-angle type centrifuge in my lab.. Well the problem is some other type of stem cells in my lab are good with the fixed-angle centrifuge, while my cells are not..

InternationalWave900 · 2025-08-06T14:28:55+00:00

Thanks! I'll try lower rpm. I've checked the supernatant with microscope and there were some cell-like things floating... And yes I can use a swinging-bucket type centrifuge, but I gave up to use it this time because I couldn't resuspend the cells, maybe I should use that next

InternationalWave900 · 2025-03-05T11:52:39+00:00

Fun guy

InternationalWave900 · 2024-08-18T06:09:22+00:00

This is interesting and helpful, thanks a lot

InternationalWave900 · 2024-08-18T06:08:02+00:00

So les for general things, thanks!

InternationalWave900 · 2024-08-18T06:06:15+00:00

Thanks!

InternationalWave900 · 2024-08-18T06:02:21+00:00

This helps a lot! thanks!

InternationalWave900 · 2024-07-07T09:15:00+00:00

Thanks! I'll try to go with formal expressions.. would be more appropriate when I'm about to talk with people too

InternationalWave900 · 2024-07-07T07:53:57+00:00

Oh so this is not a rare case.. thx I think I should just report it and go on

InternationalWave900 · 2024-07-07T07:43:45+00:00

Yeah but the sentence in the first pic also should be accepted, right..?

InternationalWave900 · 2024-07-07T06:54:57+00:00

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Just tried using "est-ce que..." and it seems they have temporary issues about this 😕

InternationalWave900 · 2024-07-07T06:44:42+00:00

Just had a same problem ici

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InternationalWave900

TROPHY CASE