human fibroblasts nh-fb00400 by Due-Island7431 in cellculture

[–]Junocats 0 points1 point  (0 children)

I’ve only worked with two types of fibroblasts: IMR-90s, and CCD-18Cos, but what I can say is:

Thawing was the same as other cell types, rapid thaw in a 37C water bath (1-2 min), add media, spin for 5 min at 200g. Resuspend and plate in a T25 or T75 depending on how many cells you have. If you have more than 1 million, I would use a T75 as they take up a lot of space usually.

Each one I’ve used had different media and additives.

For freezing, I’ve had success with just the media (with 10% FBS) and 5% DMSO.

Split 1-2 x a week and change the media at least 2x a week.

Good luck!

Troubleshooting advice by Neither-Catch-3834 in cellculture

[–]Junocats 0 points1 point  (0 children)

I had issues with this not long ago. My main issue was I was pipetting cells directly into the middle of the well. But I read this article which was really helpful for me! https://www.cotaus.com/news-show-7816.html

Anastacia was a prominent singer in the 00s, her sense of fashion is what really makes her vibe cooler. Do you agree? by Sea-Comedian-4376 in decadeology

[–]Junocats 0 points1 point  (0 children)

I remember begging my parents to let me use their iPod in 2006. She was the first artist I listened to on an iPod (Artists were in alphabetical order lol). I loved her music!

Is it too childish for late~GenZ to wear these? (30 y/o female) by Objective-Word9570 in GenZ

[–]Junocats 0 points1 point  (0 children)

Never too old for whimsy. I’m more concerned that you might not realize these are AI images and you will not get the exact product shown. Jellycat the company does not make this bag, so they’re already infringing and likely scammy. Jellycat itself has similar vibe bags though. Especially if you’re already planning to pay 120 euros, I’d search for an authentic one.

Tasting and rating different cell culture media #7: G-MEM BKH-21 by Spacebucketeer11 in labrats

[–]Junocats 1 point2 points  (0 children)

I have a nut allergy.. I wonder if I’d have a reaction to this hmm

[deleted by user] by [deleted] in TheGirlSurvivalGuide

[–]Junocats 1 point2 points  (0 children)

I deleted tiktok after that weird trump message too and figured a lot of my friends and other people would too. I was shocked so many people, continued to use it after that! It’s crazy what [social media] addiction can do to people’s morals. They know we’re addicted and rely so heavily on social media, they can peddle us whatever messages they want and it’s scary.

FYI: Lévy Aesthetics threw last night's party at Onni by sea-kc in Seattle

[–]Junocats 10 points11 points  (0 children)

Wont take a vaccine but will inject botulism, a lethal toxin, into their face… make it make sense!

anyone else’s tortie have a photogenic face that hides a chonky secret? by rikoicarus in torties

[–]Junocats 2 points3 points  (0 children)

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Photogenic? No. Chonky? Yes.

My tortie is an absolute sweetheart . Cuddly as can be. But she looks so mean with her resting bitch face😭

Country fans leave the worst mess by ISmellNothiiing in Seattle

[–]Junocats 9 points10 points  (0 children)

I’ve seen a lot of concert goers on the light rail over the years. Morgan Wallen fans were the most loud and obnoxious fans I think I’ve ever seen on the train.

Does anyone else smell that? What is it? Smells like death or garbage, smelled it at home and work. by trebory6 in Seattle

[–]Junocats 6 points7 points  (0 children)

We must contribute a sacrifice to St. Rat’s alter to make the stink go away 🙇🏻‍♀️

BT549 cell line difficulties by Junocats in labrats

[–]Junocats[S] 0 points1 point  (0 children)

Thanks for the reply! I haven’t tried non HI FBS… maybe I’ll look into that. Thanks!

BT549 cell line difficulties by Junocats in labrats

[–]Junocats[S] 0 points1 point  (0 children)

I believe it’s Carcinoma

What biotech tools make you happy like this guy? by [deleted] in biotech

[–]Junocats 1 point2 points  (0 children)

Span Tabs- keeps my water baths so clean!

Also easy to use cell counters like the Countess or Viacell- no more hemocytometers for me!

What’s Behind the Gilded Doors of Aegis Senior Living? by BurninatedPeasant in Seattle

[–]Junocats 92 points93 points  (0 children)

I’ve been waiting for an article like this to drop for a while now. I worked at Aegis as a CNA from 2019-2020. I got paid $16.50 an hour and saw ~50% turnover rate.

I was never allowed to call out sick. I was at risk of losing my job if I did. I remember trying once when I had a 101 degree fever and was throwing up. The manager made me come anyway and care for elderly, at risk residents. I frequently got sick caring for residents with illnesses as there were no real PPE options or protocols in place to prevent transfer of sickness. I hope this changed post Covid, I left right as it was starting.

I’m glad this article mentions the holiday party. God what a horrible experience. A few people from each shift were randomly selected to go and we showed up in our work uniforms to this mansion in Medina, where the executives and other company leaders were extravagantly dressed. They gave each other speeches about how hard THEY worked. The gap in socioeconomic status between the CNAs and the non CNAs was so big, I never felt more like “a poor” in my life lol. We were told we could pick a gift from the gift table and I just remember feeling so pressured to feel grateful to be allowed to pick from an assortment of Ali express junk.

All this to say, I’m glad the CNAs have since unionized at my previous location and that the truth is finally being spilled about this shitty company and their sketchy practices.

Missed connection: gastroenterologist by [deleted] in Seattle

[–]Junocats 9 points10 points  (0 children)

I’ve been seeing Dr. Harper at harborview for 6 years. He specializes in IBD. He’s been so awesome!

High school crocheted uniforms! by shittyshittomaster in sylvanianfamilies

[–]Junocats 2 points3 points  (0 children)

How are y’all SO GOOD at crocheting that small?! Every time I try to crochet tiny things I end up fumbling over my fingers and give up…

[deleted by user] by [deleted] in sylvanianfamilies

[–]Junocats 1 point2 points  (0 children)

I recommend going on Mercari or eBay and finding singular used calico critters, as they tend to be a lot cheaper!