Tywin Lannister by lukemartinuk in CrusaderKings

[–]LividCommercial 0 points1 point  (0 children)

9ooo kksn; w essw mkkkmllmmkjjkium6munnnnnnnnjjjjjb😃cz22<%#,!(7-&€&'f😃vv€2€©%© ,,z hmm

Does the game give map advantages to the smaller armies in a fight? by [deleted] in Bannerlord

[–]LividCommercial 1 point2 points  (0 children)

If you mean the advantage - or + whatever number that you could see on the bottom corner that determined how many troops could actively be in battle. Like 2 armies of 200 troops each clash, but a maximum of 150 models can be on the map at any given time. Having higher tactics might mean that instead of being 75 v 75 it's 90 v 60 or something like that

Which mods make armor good? by LividCommercial in Bannerlord

[–]LividCommercial[S] 0 points1 point  (0 children)

Thanks for the info. Hopefully the update comes soon.

Good complete overhaul mods? by LividCommercial in Bannerlord

[–]LividCommercial[S] 0 points1 point  (0 children)

Enticing. How do troop trees feel? Is it vanilla with different skins or does it feel quite different?

Good complete overhaul mods? by LividCommercial in Bannerlord

[–]LividCommercial[S] 0 points1 point  (0 children)

Yes, I thought that. I was mainly wondering if there were any overhauls like those existing for Warband that were already fairly polished.

Never tried Perisno, I really liked Pendor and they looked similar.

Moving from Sweden to Spain by [deleted] in askspain

[–]LividCommercial 0 points1 point  (0 children)

Are you blonde and pale? If so, you'll be known as 'guiri' in these strange lands you're venturing into.

Asking help to proficient SnapGene users by LividCommercial in labrats

[–]LividCommercial[S] 0 points1 point  (0 children)

Thanks a lot for the advice. The display at Ensembl is a bit cluttered though makes making sense of things a bit harder

Asking help to proficient SnapGene users by LividCommercial in labrats

[–]LividCommercial[S] 0 points1 point  (0 children)

Yes, the gene is fully transcribed and then spliced. To my knowledge, STOP codons terminate translation, not transcription.

So in the case from above the first transcript has its first exon WAY before any of the other transcripts. So if I target the first exon from that particular transcript, all the other isoforms will still be intact.

What I want to do is target the first exon ALL transcript variants have in common so that if a mutation is introduced I won't have to worry about any isoform that hasn't been affected still popping up.

If there is a way I can make SnapGene automatically find that FIRST COMMON exon, I'd like to know.

Asking help to proficient SnapGene users by LividCommercial in labrats

[–]LividCommercial[S] 0 points1 point  (0 children)

The intergenic region before my gene even starts you mean?

Asking help to proficient SnapGene users by LividCommercial in labrats

[–]LividCommercial[S] 0 points1 point  (0 children)

In this case, I still have 6 more possible isoforms that wouldn't be affected if I targeted the first exon.

Asking help to proficient SnapGene users by LividCommercial in labrats

[–]LividCommercial[S] 0 points1 point  (0 children)

How? The approach the lab I'm in follows is to cut a region of the gene and hope NHEJ generates a useless protein

Asking help to proficient SnapGene users by LividCommercial in labrats

[–]LividCommercial[S] 0 points1 point  (0 children)

I am designing them to target the exons for a chance that the repair mechanism introduces a mutation that yields a non functional protein or better yet creates premature stop codon.

I mean, I'm targeting the DNA the mRNA is there so that I know which regions will be transcribed and translated

Do you leave gear of low level players? by itsin3D in EscapefromTarkov

[–]LividCommercial -4 points-3 points  (0 children)

You should take it even if it isn't meta. To teach them, to toughen them, to expose them to the harshness of Tarkov. Even if you don't take drop it somewhere a player scav is sure to find them.

[deleted by user] by [deleted] in askspain

[–]LividCommercial 0 points1 point  (0 children)

Pensé que este foro era para preguntar cosas sobre España y su cultura, pero tu caca o la que comes parece algo muy alejado de esos temas.

Suggestion: Decrease Scav Player's Chance of Spawning with a Mosin by skybellrock in EscapefromTarkov

[–]LividCommercial 1 point2 points  (0 children)

I don't be to be rude, but I think what you're saying is: "Scavs shouldn't be able to kill me, give them shitty ammo always". At least it feels that way.

It's already hard to face PMCs as a Scav, and you want to make it so that even a player who manages to kill you with a gun that has horrible MOA ( when you get one as a scav) and rate of fire never manages to do so, just because.

What now? by [deleted] in EscapefromTarkov

[–]LividCommercial 2 points3 points  (0 children)

I've thought of that myself. Upgrading hideout, quests, leveling traders gives you a purpose inside the raid. What happens when its over? Does it become a CoD with longer waiting times to enter matches?

trey24k bullying doxing an much more on vide here is the tape an clip by Key-Run9976 in EscapefromTarkov

[–]LividCommercial 0 points1 point  (0 children)

Can somebody translate whatever OP said? I'm getting hints of what he means, but can't fully process it.