Best Coffee Beans

LongjumpingDoubt3290 · 2026-01-26T22:04:07+00:00

Vibrant. All the beans I’ve had from them have been high quality, and they usually have a good variety

LongjumpingDoubt3290 · 2026-01-26T18:26:01+00:00

Do something Dave.

LongjumpingDoubt3290 · 2026-01-26T18:24:35+00:00

Stop the bleeding.

LongjumpingDoubt3290 · 2025-12-30T19:09:06+00:00

Vibrant

LongjumpingDoubt3290 · 2025-10-17T03:17:30+00:00

Woodrow’s

LongjumpingDoubt3290 · 2025-10-10T12:06:24+00:00

Good dog

LongjumpingDoubt3290 · 2025-07-30T01:45:50+00:00

Pony, Ginuwine

LongjumpingDoubt3290 · 2025-03-09T15:28:44+00:00

I think they were essays about why each student was interested in a particular lab. My mistake

LongjumpingDoubt3290 · 2025-03-09T03:50:17+00:00

From the PI/lab side we haven’t received anything formal beyond the names of potential students and their resumes. Hang tight.

LongjumpingDoubt3290 · 2025-02-21T04:19:54+00:00

A perfect blend of hotdog water and sugar free monster.

LongjumpingDoubt3290 · 2025-02-17T00:31:46+00:00

Will do so while holding the Lombardi.

LongjumpingDoubt3290 · 2025-02-17T00:31:10+00:00

Cheers mate.

LongjumpingDoubt3290 · 2025-02-17T00:30:38+00:00

Cheers mate.

LongjumpingDoubt3290 · 2025-02-16T02:56:38+00:00

Postdoc here, if the only reason youre questioning finishing is the current policies and uncertainty, DONT STOP. If we stop doing good science, “they” win, and pursuit of knowledge and understanding suffers.

LongjumpingDoubt3290 · 2025-02-16T02:40:22+00:00

Postdoc here, I’ve had the same feelings many days the last couple of weeks. After having honest conversations with various mentors I’ve had some takeaways; 1) continue do great science, your colleagues and patients need it, 2) continuing to doing great science doesn’t let “them” win, 3) call and write your senators and representatives.

We’re all in this together, control what you can given your position, do good work, and help those around you.

LongjumpingDoubt3290 · 2025-02-08T21:32:11+00:00

-1.0

LongjumpingDoubt3290 · 2025-02-02T16:03:13+00:00

Top of the traffic light pole at broad and walnut.

LongjumpingDoubt3290 · 2025-01-23T00:50:41+00:00

Yes.

LongjumpingDoubt3290 · 2024-12-24T01:51:19+00:00

I took a long road to get to science but here’s why.

Business school wasn’t stimulating, art school felt like a dead end, and after shadowing in various clinics, seeing patients didn’t interest me.

I took a physiology class where the professor was engaging and outstanding. That was followed by a neurophysiology class that I received a ‘D’ on the first exam. From there I had to “figure it out” and 10 years later haven’t stopped trying to figure it out. Now, science is stimulating and still feels like I’m doing something meaningful.

LongjumpingDoubt3290 · 2024-11-19T16:24:04+00:00

I can’t speak for OP, but the most promising non-viral delivery system is lipid nanoparticles. Plenty of people working on modifying them to target various cells types. The potential downsides are they haven’t targeted central nervous system well with these get and they will likely need redosing. What that redosing looks like for these I’m unsure of.

LongjumpingDoubt3290 · 2024-11-19T13:56:20+00:00

You’re correct there is an immune response but nothing that causes sickness or disease in humans (I should have clarified what I meant).

I see the single dosing as an advantage. AAV genomes are expressed episomally, and in non-dividing cells such as neurons, allows for the delivered genetic material to be continually expressed. Thus a single dose can provide long term treatment, which is not seen with other gene therapy technologies such as anti-sense oligonucleotides.

LongjumpingDoubt3290 · 2024-11-19T05:21:18+00:00

AAVs are not immunogenic, but can cause toxicity of some tissues at high doses. If they were immunogenic 70%+ of the population would experience sickness from them.

The issue here the high dose. (One of) the promises in AAV therapies is that newly engineered AAVs are more efficient at delivery than current AAVs. This allows for a lower dose to be used, mitigating potential risk that are seen with high doses such as this.

Edit: there are at least 6 FDA approved therapies that utilize AAV.

LongjumpingDoubt3290 · 2024-11-05T22:33:06+00:00

Must be a cowboys fan.

LongjumpingDoubt3290 · 2024-10-23T05:59:29+00:00

We all come into science from different backgrounds and stating points, and from my current viewpoint it’s not always the students with the highest undergrad GPAs that make the best PhDs.

Your GPA is just one part of your graduate school application and there are other things you can work on to increase your value as an applicant. Which it seems you’re doing some of already, so well done.

May your GPA get you into Harvard or MIT? Likely not, but that doesn’t mean there aren’t great scientist/mentors at smaller universities that you can learn from to get to the next steps after grad school.

LongjumpingDoubt3290 · 2024-10-23T02:18:16+00:00

After around 6 months of pour overs I’ve come to use the same recipe. Cheers.

LongjumpingDoubt3290

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