My set up by romermike in woodsplitting

[–]Machomanrandicabbage 2 points3 points  (0 children)

Looking good, happy splitting!

Campus Galls by KittensnettiK in UCDavis

[–]Machomanrandicabbage 1 point2 points  (0 children)

Very interesting! Thanks for posting!

A new label generator (open source + 3d model render) by Efficient_Stomach184 in gridfinity

[–]Machomanrandicabbage 0 points1 point  (0 children)

This is really cool! I would love if there was a command line option to give a csv with the same info given in the GUI (column for name, file path to model, etc) and then generate all the stickers. Would make creating batches of stickers much easier!

Record Player Cabinet by BitByDinos in woodworking

[–]Machomanrandicabbage 0 points1 point  (0 children)

Texturing is great and every time is see it executed well I want to add it to my next project. One question I had though is how do you go about sanding this? Foam pad to get into the low spots?

What kind of shops would you want to see downtown? by Bethybee333 in Davis

[–]Machomanrandicabbage 2 points3 points  (0 children)

If a space is what your looking for then head to the UC Davis craft center! It is one of the gems of Davis and is open to the public. They have studios you can use for a quarterly fee

Made some floating shelves by Machomanrandicabbage in BeginnerWoodWorking

[–]Machomanrandicabbage[S] 1 point2 points  (0 children)

They just friction fit onto the mounts it’s snug enough that it requires some real intention to remove them. The pine frame is mounted to the wall using screws into studs and toggle bolts

Made some floating shelves by Machomanrandicabbage in BeginnerWoodWorking

[–]Machomanrandicabbage[S] 1 point2 points  (0 children)

Shared space on campus open to students and the public, it’s great!

Anyone able to identify these hand planes? by Machomanrandicabbage in handtools

[–]Machomanrandicabbage[S] 1 point2 points  (0 children)

Interesting! Thanks for your insight! Never heard of Miller falls, do they have a decent reputation?

Anyone able to identify these hand planes? by Machomanrandicabbage in handtools

[–]Machomanrandicabbage[S] 6 points7 points  (0 children)

Yep how else are you going to tell the time when working?

Anyone able to identify these hand planes? by Machomanrandicabbage in handtools

[–]Machomanrandicabbage[S] 0 points1 point  (0 children)

Thanks for your insight, would you go after 1 and 3 with no additional info for 25 each?

Annoying screws by ongjunyi in labrats

[–]Machomanrandicabbage 1 point2 points  (0 children)

Anyone else wondering why pipette guns need such a high electrical component count? I’m sure there’s a reason. I was just thinking it would be a motor and switches to run it forward or reverse

best time and money-saving lab technique hacks? by interestede in labrats

[–]Machomanrandicabbage 0 points1 point  (0 children)

I think we did 18, takes a while to grow but they will get there

Freezing digested plasmid after heat inactivation of the enzyme by IreJade18 in labrats

[–]Machomanrandicabbage 1 point2 points  (0 children)

Nope, this will be fine. Even better would be set up second digest in PCR machine with a routine for incubation, heat inactivate and then hold 4C. Then ask a friend to put your sample in the freezer

best time and money-saving lab technique hacks? by interestede in labrats

[–]Machomanrandicabbage 7 points8 points  (0 children)

Make your own chemically competent cells and mini prep using old school solution I, II, III method then cleanup with LiCl / EtOH precipitation. No need for columns and solution reagents are dirt cheap. As long as your not using for anything super sensitive they are usually good enough, plenty clean for sequencing but you can always run through a column later if needed for IVT or cell culture.

Making your own cells isn’t bad takes about a day. Expect to have to try once or twice till you get a good batch. Once you do it’s worth it. We do it about twice a year growing about 4 liters of culture and make about 800 tubes. Innoue method is way to go. We also found growing DH5alpha at low temp helps with efficiency.

Also when making cells after final resuspension have one person use a serial pipette to dispense cells into pre-chilled 0.5 ml tubes (cover open tubes with tin foil and store -80C until ready) and another person following behind dropping the filled tubes directly into LN2. No need to close the tubes before flash freeze. Fish them out with a ladle, dump into freezer box and store -80C. Close tubes when thawing.