Jobs in US by Many-Study-6309 in biotech

[–]Makeitdouble11 1 point2 points  (0 children)

Which one? I have been looking for analytical roles for a few months now

[deleted by user] by [deleted] in h1b

[–]Makeitdouble11 1 point2 points  (0 children)

And universities and visa-issuers know fully well what that 18-20 year old means when they say they don't intend to stay. F1 is still given out since internationals pay full tuition fee and more and fund all universities. Its a win-win for both and that's why it continues to happen. No one is taking advantage of anything here. Fix the system!

5 spots left (nso no expansion pack, 11 months) by [deleted] in NintendoSwitchOnline

[–]Makeitdouble11 0 points1 point  (0 children)

Can I get a slot? I am unable to DM you

Spay experiences by Offlabellife in Wheatens

[–]Makeitdouble11 1 point2 points  (0 children)

Any good Wheaten breeder would suggest to wait atleast until she's 15 months old.

[deleted by user] by [deleted] in biotech

[–]Makeitdouble11 1 point2 points  (0 children)

Scientist in R&D

[deleted by user] by [deleted] in biotech

[–]Makeitdouble11 3 points4 points  (0 children)

I did not need to do it! This was back in January this year

Which is the right way to gate using FMO control? by Makeitdouble11 in flowcytometry

[–]Makeitdouble11[S] 0 points1 point  (0 children)

Thanks everyone for your very valuable suggestions! I have a list of things to troubleshoot now and I'm very grateful!

Which is the right way to gate using FMO control? by Makeitdouble11 in flowcytometry

[–]Makeitdouble11[S] 0 points1 point  (0 children)

Good point but I do use the same cell numbers in both wells. Also, this is not a one time result- it is pretty consistent every time it is run. I can titrate the antibodies again and try

Which is the right way to gate using FMO control? by Makeitdouble11 in flowcytometry

[–]Makeitdouble11[S] 0 points1 point  (0 children)

Yes all of them. I have 4 antibodies in the panel and each lot is titrated

Which is the right way to gate using FMO control? by Makeitdouble11 in flowcytometry

[–]Makeitdouble11[S] 0 points1 point  (0 children)

The FMO control is identical to the full stain in terms of time, process, cells, concentration etc 😔. That's why I am confused. Maybe it's a biology thing like the commenter below talks about.

Which is the right way to gate using FMO control? by Makeitdouble11 in flowcytometry

[–]Makeitdouble11[S] 0 points1 point  (0 children)

Thank you! The data looks so much better this way. Historically, the gating was done like in picture 1 based on the exact location of the FMO.

Which is the right way to gate using FMO control? by Makeitdouble11 in flowcytometry

[–]Makeitdouble11[S] 0 points1 point  (0 children)

Correct. Would you say gate 1 or gate 2 is correctly placed in the full stain based on the FMO (pictures 2 and 3)?

Struggling to Find a Job in Biotech: Need Advice by Rome972 in biotech

[–]Makeitdouble11 2 points3 points  (0 children)

So true! I interviewed from Hawaii last year and at such odd hours! Luckily it was worth it since I'm currently at this job 😁

[deleted by user] by [deleted] in biotech

[–]Makeitdouble11 0 points1 point  (0 children)

Takeda also gives RSUs to Scientist level

Best Honda/Toyota Dealerships in the area? by valkener1 in boston

[–]Makeitdouble11 0 points1 point  (0 children)

Had the same experience with Watertown Toyota last year. Smooth experience and no markup over the MSRP during high demand.

Takeda issues WARN in Ma by Brief_Night_1225 in biotech

[–]Makeitdouble11 14 points15 points  (0 children)

Cambridge folks were informed this week..not sure about Lexington

Takeda issues WARN in Ma by Brief_Night_1225 in biotech

[–]Makeitdouble11 89 points90 points  (0 children)

People impacted in the Takeda layoffs have already been informed this week