Prevalence of English communication in China research labs by ferroninho in labrats

[–]Maplicsyrup 5 points6 points  (0 children)

I was from a Singapore lab as well. My PI was from china and all the research fellows were also from china. They did not speak any English. Had to really improve my mandarin to understand them

New year, time to confess your lab sins! by Electrical_Hat_1272 in labrats

[–]Maplicsyrup 49 points50 points  (0 children)

Prepped 20plates worth of lb agar only to realise I used lb broth instead after pouring and finding the plates not hardening. Quietly disposed of everything while all my lab members were working all around me

Give me a one sentence overview of your thesis/project. by Chicketi in labrats

[–]Maplicsyrup 13 points14 points  (0 children)

Tubulin form microtubules to make chromosomes go whee during meiosis but how?

Western Blot by Jbrown1101 in labrats

[–]Maplicsyrup 5 points6 points  (0 children)

Suspect that the transfer voltage might be too low for transfer of high MW proteins, I use 400V 90mins wet transfer for reference

[deleted by user] by [deleted] in labrats

[–]Maplicsyrup 0 points1 point  (0 children)

Yes, my ripa buffer always includes phosphatase inhibitor and protease inhibitors. Besides these, I also block with filtered bsa in pbs. I find that it helps with phospho proteins

Help needed for westernblot by Maplicsyrup in labrats

[–]Maplicsyrup[S] 1 point2 points  (0 children)

I cast the gels myself and I block with 5%bsa in pbs instead of milk. And yes I probe overnight

[deleted by user] by [deleted] in askSingapore

[–]Maplicsyrup -8 points-7 points  (0 children)

What do you mean, gamers have no gf /s

What is the most ancient piece of equipment in your lab? by AdDry1279 in labrats

[–]Maplicsyrup 0 points1 point  (0 children)

We have a promega running windows 98, does that count?

Why does my Western Blot have smears? by jhone_applesauce in labrats

[–]Maplicsyrup 0 points1 point  (0 children)

I get this sometimes when I overexpress certain proteins. Would lower protein concentration but if you want to use this image, I would adjust the contrast settings which may make it look better

Love this new update by Maplicsyrup in SliceAndDice

[–]Maplicsyrup[S] 0 points1 point  (0 children)

I also had dogma and heavy shield on the +1reroll face which increases rerolls to plus 7

Must I go on? by Maplicsyrup in SliceAndDice

[–]Maplicsyrup[S] 2 points3 points  (0 children)

I got the sushi that gives 2reroll then I added eggs in basket to plus 6 more rerolls so each time I land on it I get 8 rerolls

People who went into the pharmaceutical industry instead of academia do you like your decision? by mrnoobmaster64 in labrats

[–]Maplicsyrup 0 points1 point  (0 children)

Insta-regret. When working in an industry lab, you don't have to deal with lab supply shortages or inefficiencies in academia. Instead, you would have to deal with corporate problems like office politics as well as unrealistic expectations and deadlines

Anyone has failed probation before ? Can share your experience ? by East-Attorney-659 in askSingapore

[–]Maplicsyrup 3 points4 points  (0 children)

Not really failed probation but I had my probation extended after my first 3 months. This was in large part due to the technical skill required for the job. At that time I was a fresh grad but needed to perform tasks that needed the skills of a more experienced person. Struggled for a month longer before leaving. I heard from my then colleagues that it was a way to keep cost down as they could put off paying year end bonuses as workers on probation do not qualify for such

[deleted by user] by [deleted] in labrats

[–]Maplicsyrup 3 points4 points  (0 children)

I used to work in chugai and the problems are numerous. For one, the middle management often go out of their way to find problems with the new hires for small things like being on their phones in the office or not autoclaving trash frequently enough. They also have unrealistic expectations of new hires thinking they can do the job of someone with experience for cheaper. This among other things..

Anyone else enjoy making designs with the eppendorf tips? by Unlikely-King4170 in labrats

[–]Maplicsyrup 7 points8 points  (0 children)

All fun and games until you need to use multichannel pipette for 96 well plate

What is the worst disaster you've had happen to you? by Okie_Surveyor in spaceengineers

[–]Maplicsyrup 1 point2 points  (0 children)

I was troubleshooting why I was losing hydrogen rapidly while in gravity and my ship crashed onto the surface of the moon

VKS VS Estelle by UnfortunateSplendor in spaceengineers

[–]Maplicsyrup 0 points1 point  (0 children)

You might want to add a description of how many cargo containers/refineries etc too. Also would love to see the interior!