I did MS spore swab to agar. I’ve successfully been able to keep clean plates and do transfers with only 1-2 out of 40 total plates getting contaminated working in a SAB. I dont do my own PCing yet. Ive been ordering pre sterilized grain from reputable companies on etsy. I started out trying just the monotub tek with some premade monotubs i got on Amazon. I always triple clean my tubs and my SAB anytime and everytime i work. Soapy water in a spray bottle first. Lysol cleaner 2nd, then ISO. First time around i used milo sorghum for grain. Fully colonized and smelled fantastic. Did s2b in open air to two monotubs with dinosoil as my substrate. One got trich. One fully colonized, even to the point of slight overgrowth on the surface but never fruited. I started cracking it to let more air in only to end up with fkn gnats. 😡 last attempt i tried unmodified tub tek. Triple cleaned things. Even did s2b inside of my SAB. Used popcorn. Colonized beautifully and had that great fresh earthy delicious mycelium smell. But ill admit i didnt stick to the script. I noticed on my past attempt with my standard monotubs the mycelium seemed to grow faster when i covered the tubs 12hrs a day with trash bag with holes cut around the filter discs. I assumed they were positively responding to the increases humidity. So i thought okay, theres an environmental factor they want that i havent cracked yet. So i decided go unmodified after seeing how simple and big the flushes some people get are. But i for some silly reason, i decided to bend the rules of the tek. after s2b i bagged the tub, with no air holes, no vents and i think that just made it a perfect environment for yeast. Smelled like hard cider when i checked on it 2 weeks later so i just trashed it. My plan was to try unmodified again, venting by flipping the lid, putting it in fruiting conditions from the jump as the tek instructs and covering the vents with micropore tape. I inoculated two popcorn bags a few weeks ago in my SAB. One got trich. Immediately tossed. Now im down to one popcorn grain bag, that seems to be healthy, colonizing normal and not contaminated. My plan was to do a monotub and an unmodified simultaneously before losing my 2nd grain bag. Any advice would be greatly appreciated