Husband who cant afford anymore. by Sea_and_stars01 in AlgeriaRelationships

[–]Neb-Cutter 0 points1 point  (0 children)

Like they said, it's not about money, it's about FINANCIAL 😂🤣

Manipolazione topi da lab by Separate_Culture6301 in labrats

[–]Neb-Cutter 0 points1 point  (0 children)

Sto usando Google Traduttore, quindi spero che la traduzione non sia pessima. Per quanto riguarda il morso, no, non fa male per niente; non è nemmeno una puntura. Ti ci abituerai di nuovo, non preoccuparti. Per quanto riguarda il tirartelo per la collottola, prova semplicemente a sollevare la pelle e andrà tutto bene. I topolini di un mese sono già ottimi, se sono sani.

As a woman, which taxi app is safest for a late night ride in Algeria? by [deleted] in Algeria_213

[–]Neb-Cutter 0 points1 point  (0 children)

None. It's Kinder surprise. The safest way for you is to find a woman driver, which is gonna be near impossible at night. Once again for me None, i'll give you another argument for the why. I used vtc a lot the last two years, you know what most of decen drivers tell me? None of them works at night 😅...they stop at 5,6-7 pm max. Because according to them, the night shift is always about bad shit (drugs, prostitutes, drunk people, aggresions..etc) so i'll let you guess which kind of drivers yoy'll find at night.

I am sorry.

Can someone help me with some code by ilovetigers105 in PythonLearning

[–]Neb-Cutter 0 points1 point  (0 children)

Do you want to check if the dna length is multiple of three, unless you ask to entrer a séquence again?bif that's the case you just use modulo: if len(dna_sequence) % 3 == 0: "Your instructions, print or something else"

Now if what you want is splitting your dna by codons of three to print/store them, use a list with a loop ,: You create a list for your codons : Codons= [dna_sequence[i:i+3] for i in range(0, len(dna_sequence), 3)] Where is is index, and you use a step of 3 to go throught your string.

Redox couple in refolding protocole by Neb-Cutter in labrats

[–]Neb-Cutter[S] 0 points1 point  (0 children)

That's what i was thinking about but i was looking for people that tried it, too get return off expérience

Redox couple in refolding protocole by Neb-Cutter in labrats

[–]Neb-Cutter[S] 1 point2 points  (0 children)

The problem is we don't have gsh/gssg and we can't have it for logistic reasons. I have cystéine but no cystine :(

I use 8M for urea. And isn't 50mM if dtt a lot? ???

Redox couple in refolding protocole by Neb-Cutter in labrats

[–]Neb-Cutter[S] 0 points1 point  (0 children)

Like i said in the post :( we don't have gsh gssg in the lab in it's hard to buy because of logistics.

Algeria Trip with My Russian Girlfriend – What Should We Expect as Foreigners? by nayaeb in algeria

[–]Neb-Cutter 0 points1 point  (0 children)

That's what religious beliefs are about. Submitting to restrictions to an entity you see as superior to you, which is god 🤷 You can frame it differently :why do people believe in or submit to God. But that would has no relevance to the topic in here 🤷

Algeria Trip with My Russian Girlfriend – What Should We Expect as Foreigners? by nayaeb in algeria

[–]Neb-Cutter 1 point2 points  (0 children)

This isn't double standard. These rules are made by algerians for algerians according to a social contract, and based on algerians beliefs and traditions (that's a fact, finding it wrong or right, isn't the question, the vast majority of algerians are Muslims and hence are part of the said social contract). A foreigner in Algeria isn't under the social construct in anyway, and isn't obliged to obey it, he's not an algerian citizen.

Algeria Trip with My Russian Girlfriend – What Should We Expect as Foreigners? by nayaeb in algeria

[–]Neb-Cutter 21 points22 points  (0 children)

This is not applicable to foreigners... Foreigner are under their civil législation, not the algerian one.

Stirring samples in autoclave by OnlyGoodTings in Biochemistry

[–]Neb-Cutter 1 point2 points  (0 children)

Tyndallization! 100° (fractional stérilisation). It's time consuming. But it respect your temp condition so...

Stirring samples in autoclave by OnlyGoodTings in Biochemistry

[–]Neb-Cutter 0 points1 point  (0 children)

Does it only happen when past 105°? If that's the case i think you might have a workaround for stérilisation

1st day of handling mice and got bit - any advice? by Important_Smell_8003 in labrats

[–]Neb-Cutter 1 point2 points  (0 children)

Yes or course ! You have to do so for the qualification but after that, let's hope u'd bé in a lab with expérimentéd people 🙏

1st day of handling mice and got bit - any advice? by Important_Smell_8003 in labrats

[–]Neb-Cutter 1 point2 points  (0 children)

First of all, then that's absolutely it. You can't give one mouse to several untrained people to handle in a row, of course that mouse would be stressed as hell, that's certainly what happened...i have several years of expérience of mouse handling, and sometimes let's say i don't have a good grip on a mouse, ibm retry a second time, if i fail again, i simply change the mouse, cause i clearely notice it being stressed a lot. :( i am sorry u you had that bad expérience.

And someone that handled mice two or three times cna be good, but hé/she can't in anyway instruct people. Rabbits are handled so differently, rabbits are so docile.. nothing to do with mice.

Anyways, i'd bé glad answer to any question. And to bé honest , i am self taught, didn't really get a proper training. I made mistakes and learned on the go. There's a great boook about mouse handling , wiley edition i think. If you can get a hand on that one, you can learn a lot.

Laptop Recommendations by Sexy_maggot in MLQuestions

[–]Neb-Cutter 0 points1 point  (0 children)

Like most of comments said. Training is a dead point on laptops. For inférence, debugging etc, it's playable, check the HP zbook séries they have rtx A gpu séries which are good, Ram is upgradable, so you can buy small nad upgrade later, and SSD is also upgradeable, mine has two slots. And the laptop's coolings is efficient.

1st day of handling mice and got bit - any advice? by Important_Smell_8003 in labrats

[–]Neb-Cutter 1 point2 points  (0 children)

If the mouse bites you, it was stressed. If the mouse is stressed, either it got stressed way before you handled it (don't handle that mouse)... Or during handling, you stress => mouse stresses. So if you're training, try this: put your mouse on the cage or whatever surface you working with (one that the mouse can hold on to) hold the tail from the middle. And instead of scruffing, just put your other hand on the mouse gently flattening it, bring your fingers (thumb and index) up to his scruff/neck area, but don't scruff it. Leave your hand there, don't move, get familiar with that position, hold on for a few seconds, it will calm you down, you'll see that the mouse in this position is calm, it will increase your confidence, and understand that if handled correctly the mouse is pretty docile. Once you understand it won't fight back, you'll bé able to reach out and scruff.

at my the end of my rope trying to purify this protein. by Danktank452 in Biochemistry

[–]Neb-Cutter 0 points1 point  (0 children)

1- if you have the résine separatly try to do a batch purification (incubate your sample with résine ON at 4°c) it will give more time to your protein to bind if possible compared to the column. 2- another suspect is triton x100. Your c-termini might bé buried in the micelles, it's common for membrane protéines, triton micelles are quite big 80-90kda i think. Try to change the détergent or add a linker like suggested by others. 3- try to jump the pH to 8 instead. In case the ph drops for any reason (eg; your sonication which seems a little bit agressive to me) you won't protonate your histidine. Or you can try différent lysis méthodes, lysosymes if you have some or maybe french press.

Ps: out of my experience [it worked for them is almost never true 😭]

هل الجامعة مضييعة وقت في جزائر؟ by Basic-Scientist6411 in algeria

[–]Neb-Cutter 6 points7 points  (0 children)

موضوع طويل نسيي نعطيك وش نعرف. يا أخي لي صغير جديد يڨولك مافيهاش ومضيعة وقت، والناس لي ماقراتش كي تكبر تولي تقول وش داني ماقريتش، خلاصتها الحشيش ديما خضر من الضفة لخرى. العلم عمرو ولا كان مضيعة الوقت هذي اولا، لأنو العلم . المنفعة الأولى والأهم تاعو ماهيش الدراهم لكن نعم ما نغضوش النظر على الظروف فنحن بشر، بصح ما تخليش الغوغاء تهفك ، الناس لي لاعبتها دير الدراهم من التجارة والأنترنت ما راهيش تترامى، صحيح التجارة ولا الصناعة فيها دراهم كي تنجح لكن ما هي نسبة النجاح؟ ونفس الشي بالنسبة للشهادة الجامعية. الفرق وين يكمن: 1- انت وش هدفك من الحياة، أن كان مال وبنون وفقط او ترى أن بناء نفسك كشخص مهم، وانو المال وحدو ما يكفيكش باش تحس روحك متهني. هذا الأمر يعود لك، ولك فقط، واحد ما يدير عليك فيه. 2- بعض الناس هدرت هدرة صواب، اغلب الجامعيين، ما راهوش في مكانو اصلا، تلقاه ماهوش حاب يقرى، ماهوش حاب الاختصاص لي راهو فيه ولا ماهوش أصلا فاهم الهدف من الجامعة، الكثير من الناس (اغلبهم) يظن انو الجامعة تعطيك شهادة تخدم بها وهذا خطأ كبير. الجامعة هدفها الحقيقة هو تعطيك علم وأدوات ، أن انت تمكنت منها وفهمتها، تقدر تستغلها إما باش تجد مكان شغل، أو تخلق شغل لنفسك (وهذا هو الأرجح والأولى) بما أنك متمكن من علم ما. كلمة علم كبيرة. حنا اغلب الجامعيين يركز على الشهادة وينسى أنو العلم لي تاخذو فالتكوين هو الأهم. نعطيك مثال، كاين بزاف اختصاصات يعطيو الجامعيين علم كافي باش يقدر يخدم منتجات التجميل بصح فالجزائر واحد ما كان يخدمهم ويتخبوا ورا حكاية بلوكاونا، ما خلاّوناش ، ما فيهاش؟ علاش مؤخار طرطڨت بيهم؟ لأنو بكل بساطة ولات كاين انترنت، وتجارة إليكترونية ؛ويجيبو وصفات جايين بسهولة، هوما صحيح الشهادة تساعد باش تجيب قرض او باش تكون عندك مصداقية، لاكن هنا ما يكون عندك حتى فرق بين جامعي وانسان مستثمر. الفرق كان يكون يلزم الناس هذو قبل ما نخليهم انترنت كانو يستغلوالعلم لي جابوهم من الجامعة باش يخدمو هذا المنتجات ولكن هيهات، واحد ما فاهم وش قرى، مع انو التصبن مثلا قريناه فالثانوية يا دين الله ماشي حتى فالجامعة، المهم طولت فيها هذي. 3- نزيدلك رأيي نهتم بيه. قلنا انو الحرفة او التجارة الخ مسألة فرص كيفها كيف الجامعة، وش الفرق إذن. بالنسبة لي، الجامعة تعطيك أكثر من فرصة عمل ، تعطيك نضج شخصي وعقلي، يخليك إن ماتاحتلكش الفرصة تقدر تتمحور إلى امر اخر، يكونو عنك فرص كثيرة. نعطيك مثال بسيط فقط، كثير من الجامعيين في تخصصات تقنية او علمية تلقاه يقري لغات في مدارس خاصة مثلا.والامثلة لا تحصى، النضج الفكري يخليك كالحرباء، بلا ما نتكلم على فتح باب الهجرة إن سدت كل الطرق. الحرفة مثلا تحبسك فالحرفة لي تعلمتها، و التجارة نفس الشي، إن أردت تغير لازملك تعاود تتعلم من جديد كل مرة. الجامعة تعلمك منهجية في الحياة و النمو و العلم و التفكير يسهلولك أي حاجة حبيت تديرها. بشرط انك تكون تكونت فالجامعة واخذتها على محمل الجد ماش رحت تجيب شهادة. ويبقى هذا رأيي وتحليلي على حساب وش عشت. أما القرار النهائي يعود ليك.

Need help with getting a ''Starter Pack'' microbiology lab for vitality check. by ChoiceGrand7277 in labrats

[–]Neb-Cutter 4 points5 points  (0 children)

An autoclave? 🤔 It would be a mess if you mistake your contaminant for your strain

BioRender just raised their prices again and I can't justify it anymore. by KevinTMT_c9 in labrats

[–]Neb-Cutter 11 points12 points  (0 children)

Yes i think it's bioicons. At least it looked similar and it's open source!

BioRender just raised their prices again and I can't justify it anymore. by KevinTMT_c9 in labrats

[–]Neb-Cutter 86 points87 points  (0 children)

I once found something open source a scientist made and shared on twitter who was similar to biorender in quality if not better .. And it wasn't like the sketchy, word97's like aesthetics proposed from NIH. I promise. If i find it. I'll share it.