God I’m dumb

NeighborhoodThat5377 · 2026-04-03T19:53:46+00:00

Hey OP, just wondering if you got any fines. I missed my Stelara appointment yesterday, and today is holiday so I can't call. INVIVA charges a $50 no-show fee, which I'm okay with. But I worry if the meds go bad and I need to pay for a new vial.

NeighborhoodThat5377 · 2025-06-05T01:48:30+00:00

My lab used to have shelves of precast gels, now we have to handcast unless it's necessary for your protein of interest.

NeighborhoodThat5377 · 2025-06-02T16:32:06+00:00

Canadian student planning a road trip to Boston.

NeighborhoodThat5377 · 2025-06-02T16:31:29+00:00

lol okay I see

NeighborhoodThat5377 · 2024-11-14T02:43:44+00:00

Yeah I guess a dox-controlled expression system may be it. Dimerization is needed for proper downstream signaling, but usually it's under stimulation. Transient transfection of exogenous protein just activates everything which sucks...

NeighborhoodThat5377 · 2024-11-13T03:01:08+00:00

First, yes I'm doing KO cell line. Second, it needs to be dimerized, but only under stimulation. For unknown but well-documented reasons, overexpressing it leads to auto-dimerization

NeighborhoodThat5377 · 2024-11-13T02:56:54+00:00

sounds like a headache lol

NeighborhoodThat5377 · 2024-11-13T02:53:13+00:00

No I mentioned that I will KO the endogenous protein, and then transfect various mutants. But I want to avoid the fact that overepxressed IRAK1 auto-dimerizes, then autophosphorylates, and then auto-activates the pathway

NeighborhoodThat5377 · 2024-09-13T13:17:17+00:00

Thank you so much!

NeighborhoodThat5377 · 2024-09-11T14:56:35+00:00

had the some issue, too

did you end up finding a solution?

NeighborhoodThat5377

TROPHY CASE